马大杂种相思瓶内和瓶外生根技术研究

doi:10.7525/j.issn.1673-5102.2015.06.016

摘要/Abstract

摘要： 以增殖培养的马大杂种相思无菌苗为材料，进行瓶内生根（采用增殖培养30 d，2~3 cm的无菌苗）和瓶外生根（采用增殖培养45 d，3~5 cm的无菌苗）技术的研究，并对2种生根技术进行了经济效益的简单比较分析。结果表明：将增殖培养30 d的马大杂种相思无菌苗接入1/2MS+IBA 1.0 mg·L^-1+NAA 0.5 mg·L^-1+蔗糖30%的培养基中，生根率可达99.43%，15 d后移栽，存活率达98.0%；而将增殖培养45d的马大杂种相思无菌苗用100 mg·L^-1的IBA溶液浸泡1 h进行瓶外生根实验，生根率可达94.67%。按生产10 000株苗计算，瓶外生根的经济效益比瓶内生根的多1 288.26元，效益比较可观，瓶外生根技术可以应用于马大杂种相思组培苗的大量生产。

关键词: 马大杂种相思, 瓶内生根, 瓶外生根, 经济效益

Abstract: For improving the rooting percentage and providing an optimal technology for seedling production, we studied the rooting technology of in vitro and ex vitro of Acacia mangium×A.auriculiformis hybrid by using the proliferation seedlings as materials (in vitro rooting with the proliferation cultured for 30 d, 2-3 cm seedling; and ex vitro rooting with the proliferation cultured for 45 d, 3-5 cm seedlings), and compared the economic benefits of two techniques for seedling production. Inoculating the aseptic plants which had been multiplication cultured for 30 d to 1/2MS with combination of 1.0 mg·L^-1 IBA, 0.5 mg·L^-1 NAA, 3% sucrose could get the highest rooting rate of 99.43%, and the survival rate was 98.0% after transplanted to the greenhouse 15 d. However, when immersing the aseptic seedlings which had been multiplication cultured for 45 d with 100 mg·L^-1 IBA for 1 hour, the rooting rate was 94.67%. For producing 10 000 seedlings, the economic benefit of ex vitro rooting was more than RMB 1 288.26 Yuan than that of in vitro rooting when the benefit was considerable. The ex vitro rooting technique was more available for the seedling production of Acacia mangium×A.auriculiformis.

Key words: Acacia mangium×A.auriculiformis, in vitro rooting, ex vitro rooting, economic benefit

中图分类号:

施琼1；胡峰2；黄烈健1*. 马大杂种相思瓶内和瓶外生根技术研究[J]. 植物研究, 2015, 35(6): 891-897.

SHI Qiong1；HU Feng2；HUANG Lie-Jian1*. In Vitro and ex Vitro Rooting Technology Comparison of Acacia mangium×A.auriculiformis hybrids[J]. Bulletin of Botanical Research, 2015, 35(6): 891-897.

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