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植物研究 ›› 2001, Vol. 21 ›› Issue (4): 611-614.

• 论文 • 上一篇    下一篇

以叶盘为外植体的白桦的再生

石福臣, 孔瑾, 吴双秀, 聂明珠, 祖元刚   

  1. 东北林业大学森林植物生态学开放研究实验室, 哈尔滨 150040
  • 收稿日期:2001-09-16 出版日期:2001-12-15 发布日期:2016-06-14
  • 作者简介:石福臣(1966-), 男, 教授, 从事分子生态学研究。
  • 基金资助:
    国家自然科学基金资助项目(39870634)

REGENERATION OF BETULA PLATYPHYLLA BY LEAF EXPLANT

SHI Fu-chen, KONG Jin, WU Shuang-xiu, NIE Ming-zhu, ZU Yuan-gang   

  1. Open Research Laboratory of Forest Plant Ecology Northeast Forestry University, Harbin 150040
  • Received:2001-09-16 Online:2001-12-15 Published:2016-06-14

摘要: 从不同的激素组成(BA, K T, 2, 4~D, NAA, GA3)、基本培养基(MS, WP)、外植体放置的方向性进行了实验, 建立了以白桦叶盘为外植体的再生系统。当叶盘向轴面朝下放置在培养基上时, 三周后, 从叶盘边缘生出不定芽。不定芽的诱导率为64%, 平均每片叶盘可生出6 个不定芽。叶盘再生系统的建立为白桦的遗传转化提供了前提。

关键词: 白桦, 再生, 叶盘

Abstract: The regeneration system of birch Betula platyphylla by leaf explant was established. Different kinds of hormones (BA, KT, 2, 4-D, NAA, GA3), basal media (MS, WP), explants orientation were experimented. The leaf explant was placed on medium with the adaxial face downwards. After three weeks, adventitious buds sprouted from the edge of leaf explants. The percentage of the adventitious shoot induction is 64%. The number of adventitious shoots incuced per leaf is 6.The regeneration system can be potentially used for genetic transformation of white birch.

Key words: White Birch, Regeneration, Leaf explant