枣ZjEB1c基因在果形调控中的表达特征与功能分析

doi:10.7525/j.issn.1673-5102.2026.05.011

摘要/Abstract

摘要：

为明确枣（Ziziphus jujuba）EB1家族成员ZjEB1c在果实形状建成中的作用，对京枣39基因组中的EB1家族成员进行了鉴定，并分析了其在7个枣品种7个发育时期中的表达特征；同时构建ZjEB1c过表达载体，在番茄（Solanum lycopersicum）中开展异源遗传转化，结合果实表型观察与转录组测序分析其调控作用。结果表明：枣中共鉴定出2个EB1家族成员，即ZjEB1a和ZjEB1c。ZjEB1c在果实发育中期（开花后50~78 d）表达上调，且在长果型品种中的表达量较高。3个独立转基因番茄品系果实的平均果形指数由野生型番茄果实的0.928提高至1.117~1.168，纵向基部中果皮单位面积细胞数量明显增加。转录组比较分析共鉴定出744个共同差异表达基因，主要富集于激素信号转导及细胞壁/果胶代谢相关通路。综合分析表明，ZjEB1c与果实纵向伸长密切相关，并暗示其可能通过激素信号转导和细胞分裂相关过程参与果实形状形成。

关键词: 枣, 果实形状, 微管相关蛋白, ZjEB1c基因

Abstract:

To clarify the role of the jujube（Ziziphus jujuba） EB1 family member ZjEB1c in fruit shape formation， EB1 family members in the Jingzao 39 genome were identified， and their expression patterns were analyzed across seven developmental stages in seven jujube cultivars. Meanwhile， an overexpression vector of ZjEB1c was constructed and heterologously transformed into tomato（Solanum lycopersicum）， and its regulatory effects were investigated through fruit phenotypic observation and transcriptome sequencing. The results showed that two EB1 family members， ZjEB1a and ZjEB1c， were identified in jujube. ZjEB1c was upregulated during the middle stage of fruit development（50-78 days post-anthesis）， and its expression level was higher in long-fruit cultivars. Compared with the wild type， the average fruit shape index of three independent transgenic tomato lines increased from 0.928 to 1.117-1.168， and the number of mesocarp cells per unit area increased significantly at the longitudinal base. 744 commonly differentially expressed genes were identified through comparative transcriptome analysis， which were mainly enriched in hormone signal transduction， cell wall and pectin metabolism-related pathways. Comprehensive analysis indicated that ZjEB1c was closely associated with fruit longitudinal elongation and suggested that it participated in fruit shape formation through the processes related to hormone signal transduction and cell division.

Key words: jujube, fruit shape, microtubule-associated protein, ZjEB1c

中图分类号:

S665.1

付文清, 马荣申, 任悦, 李梓萌, 王旭, 薄文浩. 枣ZjEB1c基因在果形调控中的表达特征与功能分析[J]. 植物研究, 2026, 46(5): 899-912.

Wenqing FU, Rongshen MA, Yue REN, Zimeng LI, Xu WANG, Wenhao BO. Expression Characteristics and Functional Analysis of the Jujube Gene ZjEB1c in Fruit Shape Regulation[J]. Bulletin of Botanical Research, 2026, 46(5): 899-912.

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编号 Number	引物名称 Primer name	序列（5′→3′） Sequence（5′→3′）
1	ZjEB1c-F	ATGGCGACGAACATTGGTATG
2	ZjEB1c-R	CTAGTAGGTCATAAGAGCTG
3	pBI121-ZjEB1c-F	GAGAACACGGGGGACTCTAGATGGCGACGAACATTGTATGATGGATTC
4	pBI121-ZjEB1c-R	GGGAAATTCGAGCTCGGTACCTAGTAGGTCATAAGAGCTGATCCACTG
5	35S	TTCATTTGGAGAGAACACGGGGGAC
6	NOS	CAAGACCGGCAACAGGATTCAATC

编号 Number	基因ID Gene ID	皮尔逊相关系数 Pearson correlation coefficient	推测功能 Putative function
1	101259852	0.999 089	内切葡聚糖酶16 Endoglucanase 16
2	101253063	0.999 089	色氨酸N-单加氧酶CYP79A68样蛋白 Tryptophan N-monooxygenase CYP79A68-like
3	101260874	0.994 657	核糖核酸酶3样蛋白2样蛋白 Ribonuclease 3-like protein 2-like
4	101256020	0.993 607	丝氨酸/苏氨酸蛋白激酶STY17 Serine/threonine-protein kinase STY17
5	543580	0.993 285	ER6蛋白 ER6 protein
6	101257490	0.992 775	蛋白转运蛋白SEC16B同源蛋白 Protein transport protein SEC16B homolog
7	101262229	-0.992 040	可能的6-磷酸葡萄糖酸内酯酶4（叶绿体） Probable 6-phosphogluconolactonase 4，chloroplastic
8	101258554	0.992 026	受体样蛋白14 Receptor-like protein 14
9	101055571	0.991 905	钙调蛋白结合转录因子SR1L Calmodulin-binding transcription factor SR1L
10	101264910	-0.990 710	30S核糖体蛋白S17（叶绿体） 30S ribosomal protein S17，chloroplastic
11	101258235	0.988 833	I型肌醇多磷酸5-磷酸酶12样蛋白 Type I inositol polyphosphate 5-phosphatase 12-like
12	101246721	0.988 804	Scarecrow样蛋白14 Scarecrow-like protein 14