一氧化氮参与盐胁迫下长春花酚类代谢的调控研究

doi:10.7525/j.issn.1673-5102.2021.04.019

摘要/Abstract

摘要：

为研究外源一氧化氮（NO）调控盐胁迫下长春花中酚类化合物的响应，采用液相色谱—质谱联用（LC-MS）技术靶向分析梯度浓度硝普纳（SNP）处理对盐胁迫下长春花幼苗根、茎、花、叶4个部位中酚类化合物组分及含量水平的变化。结果共鉴定出L-苯丙氨酸和18种酚类物质，C6C1类5种、C6C3类5种、C6C3C6类8种，其中原儿茶酸、绿原酸、槲皮素在长春花根、茎、花、叶4个部位中均存在；不同浓度SNP处理后长春花不同部位酚类化合物响应积累明显不同，其中C6C1和C6C3小分子酚酸类化合物主要积累在根和茎中，C3C6C3类主要富集在花和叶中；L-苯丙氨酸在茎、叶中相对含量较高，盐胁迫下茎中含量显著升高，且随外源NO浓度增大呈下降趋势。外源NO影响盐胁迫下植物器官中酚类化合物的积累和变化，其中根和茎响应敏感，从种类和相对含量的角度，茎和叶更适合检测酚类化合物。

关键词: 盐胁迫, 一氧化氮, 长春花, 代谢组, 酚类

Abstract:

In order to uncover the response of phenolic compounds of the Catharanthus roseus under salt stress regulated by exogenous nitric oxide（NO）， the ingredient and content of phenolic metabolites in roots， stems， leaves， flowers of the C. roseus were detected using Liquid Chromatography-mass spectrometry（LC-MS） technology after being treated with different concentrations of Sodium Nitroprusside（SNP） under salt stress respectively. The results showed that 5 kinds of C6C1 phenolic compounds including vanillic acid， p-Hydroxybenzoic acid， Syringic acid， Protocatechuic acids and Gallic acid， and 5 kinds of C6C3 phenolic compounds including trans-p-Hydroxycinnamic acid， Chlorogenic acid， Ferulic acid， Cinnamic acid and p-Coumaric acid and 8 kinds of C6C3C6（flavonoids） including Genistein， Petunidin， Naringenin， Quercetin-3-O-rhamnoside， Myricitrin， Quercetin， Kaempferol and Rutin and L- phenylalanine were identified respectively. Among them， quercetin， chlorogenic acid， protocatechuic acids were all in roots， stems， leaves and flowers. The responsive accumulation of the phenolic compounds in different tissues of C. roseus were significantly different under different concentrations of SNP treatment respectively. The small molecular phenolic acids including C6C1and C6C3 were mainly accumulated in roots and stems and C3C6C3 phenolic compounds were enriched mainly in surface leaves and flowers respectively. There were more L-phenylalanine in stem and leaf than in root and flower， and the relative content of L-phenylalanine in stem significantly increased under salt stress， but it showed a downward trend with the increase of NO concentration. Exogenous NO affected the accumulation and change of phenolic compounds in C. roseus under salt stress， and the roots and stems were more sensitive to the response. From the perspective of species and relative content， the stems and leaves were more suitable for the detection of phenolic compounds than others.

Key words: slat stress, nitric oxide, Catharanthus roseus, metabolome, phenolic compounds

中图分类号:

Q945.78

赵晓菊, 张奕婷, 刘佳, 刘洋, 唐中华. 一氧化氮参与盐胁迫下长春花酚类代谢的调控研究[J]. 植物研究, 2021, 41(4): 633-640.

Xiao-Ju ZHAO, Yi-Ting ZHANG, Jia LIU, Yang LIU, Zhong-Hua TANG. Regulation of NO in the Phenolics Metabolism of Catharanthus roseus under Salt Stress[J]. Bulletin of Botanical Research, 2021, 41(4): 633-640.

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参考文献 31

1	Morton M J L，Awlia M，Al-Tamimi N，et al.Salt stress under the scalpel-dissecting the genetics of salt tolerance［J］.The Plant Journal，2019，97（1）：148-163.
2	Zhu J K.Abiotic stress signaling and responses in plants［J］.Cell，2016，167（2）：313-324.
3	Li X L，Pan Y J，Chang B W，et al.NO promotes seed germination and seedling growth under high salt may depend on EIN3 protein in Arabidopsis［J］.Frontiers in Plant Science，2016，6：1203.
4	Hung K T，Chang C J，Kao C H.Paraquat toxicity is reduced by nitric oxide in rice leaves［J］.Journal of Plant Physiology，2002，159（2）：159-166.
5	He J Y，Ren Y F，Chen X L，et al.Protective roles of nitric oxide on seed germination and seedling growth of rice（Oryza sativa L.） under cadmium stress［J］.Ecotoxicology and Environmental Safety，2014，108：114-119.
6	Tatsis E C，Carqueijeiro I，De Bernonville T D，et al.A three enzyme system to generate the Strychnos alkaloid scaffold from a central biosynthetic intermediate［J］.Nature Communications，2017，8（1）：316.
7	Miettinen K，Dong L，Navrot N，et al.The seco-iridoid pathway from Catharanthus roseus［J］.Nature Communications，2014，5：3606.
8	Stavrinides A，Tatsis E，Caputi L，et al.Structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity［J］.Nature Communications，2016，7：12116.
9	Ferreres F，Pereira D M，Valentão P，et al.New phenolic compounds and antioxidant potential of Catharanthus roseus［J］.Journal of Agricultural and Food Chemistry，2008，56（21）：9967-9974.
10	Ferreres F，Pereira D M，Valentão P，et al.Simple and reproducible HPLC-DAD-ESI-MS/MS analysis of alkaloids in Catharanthus roseus roots［J］.Journal of Pharmaceutical and Biomedical Analysis，2010，51（1）：65-69.
11	Mustafa N R，Verpoorte R.Phenolic compounds in Catharanthus roseus［J］.Phytochemistry Reviews， 2007，6（2）：243-258.
12	Kumar V A，Kumar S C，Seema S.Catharanthus roseus and purposeful exploitation of saline land［J］.Bhartiya Krishi Anusandhan Patrika，2013，28（2）：94-95.
13	Tang Z H，Liu Y J，Guo X R，et al.The combined effects of salinity and nitrogen forms on Catharanthus roseus：the role of internal ammonium and free amino acids during salt stress［J］.Journal of Plant Nutrition and Soil Science，2011，174（1）：135-144.
14	贾晓东，许梦洋，莫正海，等.薄壳山核桃酚类代谢物研究进展［J］.植物学报，2020，55（1）：106-119.
	Jia X D，Xu M Y，Mo Z H，et al.Recent advances in phenolic metabolites in pecan［J］.Chinese Bulletin of Botany，2020，55（1）：106-119.
15	Metwally A，Finkemeier I，Georgi M，et al.Salicylic acid alleviates the cadmium toxicity in barley seedlings［J］.Plant Physiology，2003，132（1）：272-281.
16	Maeda H，Dudareva N.The shikimate pathway and aromatic amino Acid biosynthesis in plants［J］.Annual Review of Plant Biology，2012，63：73-105.
17	Cui F，Sui N，Duan G Y，et al.Identification of metabolites and transcripts involved in salt stress and recovery in peanut［J］.Frontiers in Plant Science，2018，9：217.
18	Galli V，Messias R D S，Perin E C，et al.Mild salt stress improves strawberry fruit quality［J］.LWT-Food Science and Technology，2016，73：693-699.
19	Tounekti T，Khemira H.NaCl stress-induced changes in the essential oil quality and abietane diterpene yield and composition in common sage［J］.Journal of Intercultural Ethnopharmacology，2015，4（3）：208-216.
20	Liu J，Liu Y，Wang Y，et al.The combined effects of ethylene and MeJA on metabolic profiling of phenolic compounds in Catharanthus roseus revealed by metabolomics analysis［J］.Frontiers in Physiology，2016，7：217.
21	Yu B F，Liu Y，Pan Y J，et al.Light enhanced the biosynthesis of terpenoid indole alkaloids to meet the opening of cotyledons in process of photomorphogenesis of Catharanthus roseus［J］.Plant Growth Regulation，2018，84（3）：617-626.
22	赵晓菊，张丽霞，满秀玲.NO对盐胁迫下长春花种子萌发和幼苗生理代谢的影响［J］.植物研究，2018，38（5）：669-674，681.
	Zhao X J，Zhang L X，Man X L.Effects of exogenous NO on seed germination and physiological metabolism in Catharanthus roseus seedling under NaCI Stress［J］.Bulletin of Botanical Research，2018，38（5）：669-674，681.
23	赵晓菊.外源一氧化氮对盐胁迫下长春花生长及酚类代谢的影响研究［D］.哈尔滨：东北林业大学，2018.
	Zhao X J.Effects of exogenous nitric oxide on the growth and phenolics metabolism of Catharanthus roseus under salt stress［D］.Harbin：Northeast Forestry University，2018.
24	Lavee S，Harshemesh H，Avidan N.Phenolic acids-possible involvement in regulating growth and alternate fruiting in olive trees［J］.Acta Horticulturae（Netherlands），1986，179（46）：317-328.
25	Walia H，Wilson C，Condamine P，et al.Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage［J］.Plant Physiology，2005，139（2）：822-835.
26	Appeldoorn M M，Venema D P，Peters T H F，et al.Some phenolic compounds increase the nitric oxide level in endothelial cells in vitro［J］.Journal of Agricultural and Food Chemistry，2009，57（17）：7693-7699.
27	Ederli L，Reale L，Madeo L，et al.NO release by nitric oxide donors in vitro and in planta［J］.Plant Physiology and Biochemistry，2009，47（1）：42-48.
28	Wilson D J，Patton S，Florova G，et al.The shikimic acid pathway and polyketide biosynthesis［J］.Journal of Industrial Microbiology and Biotechnology，1998，20（5）：299-303.
29	Tohge T.From fruit omics to fruiting omics：systematic studies of tomato fruiting by metabolic networks［J］.Molecular Plant，2020，13（8）：1114-1116.
30	Li Y，Chen Y，Zhou L，et al.MicroTom metabolic network：rewiring tomato metabolic regulatory network throughout the growth cycle［J］.Molecular Plant，2020，13（8）：1203-1218.
31	Cole C T，Ingvarsson P K.Pathway position constrains the evolution of an ecologically important pathway in aspens（Populus tremula L.）［J］.Molecular Ecology，2018，27（16）：3317-3330.

结构类型Structure type	化合物名称Compound name	R	R1	R2	R3	R4	R5	R6
C6C1	香草酸Vanillic acid	=O	H	-OCH₃	-OH	H	/	/
	对羟基苯甲酸p-Hydroxybenzoic acid	=O	H	H	-OH	H	/	/
	丁香酸Syringic acid	=O	H	OCH₃	-OH	-OCH₃	/
	原儿茶酸Protocatechuic acid	=O	H	-OH	-OH	H	/	/
	没食子酸Gallic acid	=O	H	-OH	-OH	-OH	/	/
C6C3	对羟基肉桂酸trans-p-Hydroxycinnamic acid	-OH	H	-OH	H	H	/	/
	绿原酸Chlorogenic acid	R7	-OH	-OH	H	H	/	/
	阿魏酸cis-Ferulic acid	-OH	-OCH₃	-OH	H	H	/	/
	肉桂酸Cinnamic acid	-OH	H	H	H	H	/	/
	对香豆酸cis-p-Coumaric acid	-OH	H	-OH	H	H	/	/
C6C3C6	染料木素Genistein	H	-OH	-OH	=O	H	*	/
	木犀草苷Petunidin	H	R8	-OH	=O	R9	-OH	-OH
	柚皮素Naringenin	H	-OH	-OH	-OH	H	H	-OH
	槲皮苷Quercetin-3-O-rhamnoside	-OH	-OH	-OH	=O	R10	H	-OH
	杨梅苷Myricitrin	-OH	-OH	-OH	=O	R11	-OH	-OH
	槲皮素Quercetin	H	-OH	-OH	=O	-OH	-OH	-OH
	山柰酚Kaempferol	H	-OH	-OH	=O	-OH	H	-OH
	芦丁Rutin	H	-OH	-OH	=O	R12	-OH	-OH

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