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植物研究 ›› 2021, Vol. 41 ›› Issue (4): 588-595.doi: 10.7525/j.issn.1673-5102.2021.04.014

• 研究报告 • 上一篇    

胞外ATP对壳聚糖诱导的ROS和PAL活性变化的影响

杨德丽, 王娟娟, 庞海龙, 孙坤, 冯汉青()   

  1. 西北师范大学生命科学学院,兰州 730070
  • 收稿日期:2020-09-02 出版日期:2021-07-20 发布日期:2021-03-24
  • 通讯作者: 冯汉青 E-mail:fenghanq@nwnu.edu.cn
  • 作者简介:杨德丽(1994—),女,硕士研究生,主要从事植物生理生态方面研究工作。
  • 基金资助:
    国家自然科学基金(31870246);甘肃省高等学校科研项目(2015A-007);甘肃省重点研发计划项目(18YF1NA051);甘肃省高校基本科研业务费;西北师范大学青年创新团队项目

Effects of Extracellular ATP on the Chitosan-induced Changes in ROS Levels and PAL Activity

De-Li YANG, Juan-Juan WANG, Hai-Long PANG, Kun SUN, Han-Qing FENG()   

  1. College of Life Science,Northwest Normal University,Lanzhou 730070
  • Received:2020-09-02 Online:2021-07-20 Published:2021-03-24
  • Contact: Han-Qing FENG E-mail:fenghanq@nwnu.edu.cn
  • About author:YANG De-Li(1994—),female,seniorengineer,mainly engaged in plantphysiology research work.
  • Supported by:
    National Natural Science Foundation of China(31870246);University Scientific Research Project of Gansu Province(2015A-007);Key Research and Development Project of Gansu Province(18YF1NA051);Fundamental Research Funds for the Gansu Universities of Gansu Provincial Department of Finance;Youth Innovation Team of Northwest Normal University

摘要:

以烟草BY-2悬浮细胞为材料,探讨了胞外ATP对壳聚糖引起的活性氧(reactive oxygen species,ROS)水平和苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)活性变化的影响。结果表明,5~20 μg·mL-1壳聚糖处理导致了烟草悬浮细胞细胞内ROS水平逐渐增加;壳聚糖也导致了PAL活性的增加,其活性在15 μg·mL-1壳聚糖处理下达到峰值,此后有所降低。10~40 μmol·L-1外源ATP处理未引起烟草悬浮细胞内ROS水平和PAL活性的显著变化。细胞外ATP水平则随壳聚糖浓度的增加而逐渐下降。本文进一步分析了细胞外ATP对壳聚糖引起的ROS水平和PAL活性变化的影响。结果显示,外源施加20 μmol·L-1 ATP可以有效降低壳聚糖诱导的烟草悬浮细胞ROS水平上升,同时外源ATP也明显减缓了壳聚糖所诱导的PAL活性的上升。上述结果表明,细胞外ATP水平能够影响壳聚糖引起的ROS水平和PAL活性的变化。

关键词: 壳聚糖, 胞外ATP, ROS, PAL

Abstract:

In this experiment, tobacco BY-2 suspension cells were used as materials to investigate the effects of extracellular ATP on the chitosan-induced changes of ROS(reactive oxygen species)level and PAL(phenylalanine ammonia-lyase)activity. The results showed that the tobacco suspension cells mixed with chitosan 5-20 μg·mL-1 increased the intracellular ROS level at adose-dependent manner, and the PAL activity also increased and reached to the maximum at 15 μg·mL-1 chitosan, and then decreased slightly. There was not significant difference in ROS levels and PAL activity in tobacco suspension cells by adding 10-40 μmol·L-1 exogenous ATP. The extracellular ATP(eATP) level gradually decreased with the concentration of chitosan increased. The effects of eATP on the chitosan-induced change of ROS level and PAL activity were further analyzed. The results showed that the exogenous ATP 20 μmol·L-1 added to chitosan-induced tobacco suspension cells effectively alleviated the increases of ROS level and PAL activity in cells. These results showed that effects of extracellular ATP can affect the chitosan-induced changes in ROS level and PAL activity in cells.

Key words: chitosan, extracellular ATP, ROS, PAL

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