寡霉素处理下细胞外ATP和细胞内ATP的关系及其对细胞死亡调节作用的研究

doi:10.7525/j.issn.1673-5102.2016.06.017

植物研究 ›› 2016, Vol. 36 ›› Issue (6): 925-930.doi: 10.7525/j.issn.1673-5102.2016.06.017

寡霉素处理下细胞外ATP和细胞内ATP的关系及其对细胞死亡调节作用的研究

王庆文, 白晶月, 石岱龙, 贾凌云, 冯汉青

西北师范大学生命科学学院, 兰州 730070

收稿日期:2016-07-03 出版日期:2016-11-15 发布日期:2016-11-16
通讯作者: 贾凌云,E-mail:lingyunjia1982@126.com E-mail:lingyunjia1982@126.com
作者简介:王庆文(1992-),女,硕士研究生,主要从事植物生理生态方面研究工作。
基金资助:
国家自然科学基金（31260059，31560070）；甘肃高校基本科研业务费

Relationship between Extracellular ATP and Intracellular ATP and Its Role in Regulating the Cell Death under Oligomycin Treatment

WANG Qing-Wen, BAI Jing-Yue, SHI Dai-Long, JIA Ling-Yun, FENG Han-Qing

College of Life Science, Northwest Normal University, Lanzhou 730070

Received:2016-07-03 Online:2016-11-15 Published:2016-11-16
Supported by:
The National Natural Science Foundation of China(No.31260059,31560070);Gansu University Basic Research Service Fee

摘要/Abstract

摘要： 以烟草悬浮细胞BY-2为材料，利用F_oF₁-ATP合成酶（F_oF₁-ATPase）抑制剂寡霉素研究了细胞内ATP（iATP）对胞外ATP（eATP）水平的影响，以及施加外源ATP对iATP水平和细胞死亡的调节作用。结果表明，随着寡霉素浓度的增加（5、10、25、50 μmol·L^-1），烟草悬浮细胞的iATP含量逐渐下降，eATP含量也随之减少，且细胞死亡水平在较高寡霉素处理浓度下有明显上升。同时，随着寡霉素处理时间的增加（0.5、1、3、5 h），烟草悬浮细胞的iATP含量和eATP含量也呈现出不同程度的下降，而细胞死亡水平则有所增加。施加外源ATP能够缓解寡霉素引起的细胞iATP水平的下降和细胞死亡水平的上升。上述结果表明，eATP水平受到了iATP水平的影响，而eATP也在线粒体ATP合成受到抑制时调控iATP和细胞死亡的发生。

关键词: 胞内ATP, 胞外ATP, 寡霉素, 细胞死亡

Abstract: In BY-2 tobacco suspension cells, the effect of intracellular ATP(iATP) production on extracellular ATP(eATP) levels was studied by using oligomycin(Inhibitors of F_oF₁-ATPase). The results showed that with the increase of the concentrations of oligomycin(from 5-50 μmol·L^-1), the level of iATP and eATP were decreased, and the level of cell death were increased under higher concentration of oligomycin. With the increase of the times of oligomycin treatment(0.5, 1, 3 and 5 h), the levels of both iATP and eATP were decreased in different extents, while the level of cell death were increased. Treatment with exogenous ATP alleviated the decrease in iATP and the increase in cell death level that were caused by oligomycin, The level of eATP can be affected by iATP, and eATP can regulate the iATP production and cell death under the condition of mitochondrial oxidative phosphorylation being inhibited.

Key words: intracellular ATP, extracellular ATP, oligomycin, cell death

中图分类号:

Q945.79

王庆文, 白晶月, 石岱龙, 贾凌云, 冯汉青. 寡霉素处理下细胞外ATP和细胞内ATP的关系及其对细胞死亡调节作用的研究[J]. 植物研究, 2016, 36(6): 925-930.

WANG Qing-Wen, BAI Jing-Yue, SHI Dai-Long, JIA Ling-Yun, FENG Han-Qing. Relationship between Extracellular ATP and Intracellular ATP and Its Role in Regulating the Cell Death under Oligomycin Treatment[J]. Bulletin of Botanical Research, 2016, 36(6): 925-930.

参考文献

1. Winkler H H,Neuhaus H E.Non-mitochondrial ATP transport[J].Trends in Biochemical Sciences,1999,24(2):64-68.
2. Goh C H,Jung K H,Roberts S K,et al.Mitochondria provide the main source of cytosolic ATP for activation of outward-rectifying K⁺ channels in mesophyll protoplast of chlorophyll-deficient mutant rice(OsCHLH) Seedlings[J].The Journal of Biological Chemistry,2004,279(8):6874-6882.
3. Khakh B S,Burnstock G.The double life of ATP[J].Scientific American,2009,301(6):84-92.
4. Choi J,Tanaka K,Cao Y,et al.Identification of a plant receptor for extracellular ATP[J].Science,2014,343(6168):290-294.
5. Chivasa S,Simon W J,Murphy A M,et al.The effects of extracellular adenosine 5'-triphosphate on the tobacco proteome[J].Proteomics,2010,10(2):235-244.
6. Sun J,Zhang C L,Deng S R,et al.An ATP signalling pathway in plant cells:extracellular ATP triggers programmed cell death in Populus euphratica[J].Plant,Cell & Environment,2012,35(5):893-916.
7. Dichmann S,Idzko M,Zimpfer U,et al.Adenosine triphosphate-induced oxygen radical production and CD11b up-regulation:Ca⁺⁺ mobilization and actin reorganization in human eosinophils[J].Blood,2000,95(3):973-978.
8. Silva G,Beierwaltes W H,Garvin J L.Extracellular ATP stimulates NO production in rat thick ascending limb[J].Hypertension,2006,47(3):563-567.
9. Chivasa S,Murphy A M,Hamilton J M,et al.Extracellular ATP is a regulator of pathogen defence in plants[J].The Plant Journal,2009,60(3):436-448.
10. Chivasa S,Tome D F,Murphy A M,et al.Extracellular ATP:a modulator of cell death and pathogen defense in plants[J].Journal Plant Signaling & Behavior,2009,4(11):1078-1080.
11. Chivasa S,Ndimba B K,Simon W J,et al.Extracellular ATP functions as an endogenous external metabolite regulating plant cell viability[J].The Plant Cell,2005,17(11):3019-3034.
12. Ramirez F,Marecek J F,Kantor T V,et al.Effects of borohydride-treated oligomycins on processes of energy transduction in mitochondria[J].European Journal of Biochemistry,1982,121(2):275-279.
13. Murashige T,Skoog F.A revised medium for rapid growth and bio assays with tobacco tissue cultures[J].Physiologia Plantarum,1962,15(3):473-497.
14. Yang S W,Kim S K,Kim W T.Perturbation of NgTRF1 expression induces apoptosis-like cell death in tobacco BY-2 cells and implicates NgTRF1 in the control of telomere length and stability[J].The Plant Cell,2004,16(12):3370-3385.
15. Mcelroy W D.The energy source for bioluminescence in an isolated system[J].Proceedings of the National Academy of Sciences of the United States of America,1947,33(33):342-345.
16. Yokota A,Henmi M,Takaokao N,et al.Enhancement of glucose metabolism in a pyruvic acid-hyperproducing E.coli mutant defective in F1-ATPase activity[J].Journal of Fermentation and Bioengineering,1997,83(2):132-138.
17. Keobmann B J,Westerhoff H V,Snoep J L,et al.The glycolytic flux in E.coli is controlled by the demand for ATP[J].Journal of Bacteriology,2002,184(14):3909-3916.
18. Kim S Y,Sivaguru M,Stacey G.Extracellular ATP in plants.Visualization,localization,and analysis of physiological significance in growth and signaling[J].Plant Physiology,2006,142(3):984-992.
19. Thomas C,Rajagopal A,Windsor B,et al.A role for ectophosphatase in xenobiotic resistance[J].The Plant Cell,2000,12(4):519-533.
20. Azad A K,Ishikawa T,Ishikawa T,et al.Intracellular energy depletion triggers programmed cell death during petal senescence in tulip[J].Journal of Experimental Botany,2008,59(8):2085-2095.
21. Comelli M,Pancrazio F D,Mavelli I.Apoptosis is induced by decline of mitochondrial ATP synthesis in erythroleukemia cells[J].Free Radical Biology and Medicine,2003,34(9):1190-1199.
22. Foresi N P,Laxalt A M,Tonón C V,et al.Extracellular ATP induces nitric oxide production in tomato cell suspensions[J].Plant Physiology,2007,145(3):589-592.
23. Demidchik V,Nichols C,Oliynyk M,et al.Is ATP a signaling agent in plants[J].Plant Physiology,2003,133(2):456-461.
24. Burkeen J F,Womac A D,Earnest D J,et al.Mitochondrial calcium signaling mediates rhythmic extracellular ATP accumulation in suprachiasmatic nucleus astrocytes[J].Journal of Neuroscience,2011,31(23):8432-8440.
25. Jeter C R,Tang W Q,Henaff E,et al.Evidence of a novel cell signaling role for extracellular adenosine triphosphates and diphosphates in Arabidopsis[J].The Plant Cell,2004,16(10):2652-2664.
26. Griffiths E J,Rutter G A.Mitochondrial calcium as a key regulator of mitochondrial ATP production in mammalian cells[J].Biochimica et Biophysica Acta(BBA)-Bioenergetics,2009,1787(11):1324-1333.

[1]	李丽, 王馨, 张悦婧, 贾凌云, 庞海龙, 冯汉青. 非生物胁迫对烟草悬浮细胞胞内和胞外ATP水平的影响[J]. 植物研究, 2023, 43(2): 179-185.
[2]	杨德丽, 王娟娟, 庞海龙, 孙坤, 冯汉青. 胞外ATP对壳聚糖诱导的ROS和PAL活性变化的影响[J]. 植物研究, 2021, 41(4): 588-595.
[3]	田武英, 焦青松, 石岱龙, 孙坤, 贾凌云, 冯汉青. 胞外ATP通过H₂O₂对菜豆叶片光系统Ⅱ光化学特性的系统性影响[J]. 植物研究, 2016, 36(3): 368-373.
[4]	冯汉青；白晶月；管冬冬；贾凌云；孙坤. 胞外H₂O₂及NADPH氧化酶参与了铜胁迫对植物细胞死亡的诱导[J]. 植物研究, 2015, 35(5): 710-715.
[5]	汪矛, 崔跃华, 孙克莲. 杜仲胚乳衰退过程中程序性细胞死亡的研究[J]. 植物研究, 1999, 19(4): 401-406.

寡霉素处理下细胞外ATP和细胞内ATP的关系及其对细胞死亡调节作用的研究

Relationship between Extracellular ATP and Intracellular ATP and Its Role in Regulating the Cell Death under Oligomycin Treatment

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

本文评价