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植物研究 ›› 2013, Vol. 33 ›› Issue (5): 582-586.doi: 10.7525/j.issn.1673-5102.2013.05.014

• 论文 • 上一篇    下一篇

钝裂银莲花花粉活力测定方法的研究

胡春;刘左军*;李富香;刘凤梅   

  1. 兰州理工大学生命科学与工程学院,兰州 730050
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-09-20 发布日期:2013-09-20
  • 通讯作者: 刘左军
  • 基金资助:
     

Detection Methods for Pollen Viability of Anemone obtusiloba

HU Chun;LIU Zuo-Jun*;LI Fu-Xiang;LIU Feng-Mei   

  1. School of Life Science and Engineering of Lanzhou University of Technology,Lanzhou 730050
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-09-20 Published:2013-09-20
  • Contact: LIU Zuo-Jun
  • Supported by:
     

摘要: 以3种花色(黄色、浅黄色和白色)钝裂银莲花为研究对象,分别采用TTC染色、I2-I染色、无机酸测定和蔗糖萌发4种方法,测定了钝裂银莲花(Anemone obtusiloba)的花粉活力,同时比较了不种方法对花粉活力的测定效果,并了解了花粉活力在一天内的动态变化情况。结果表明:(1)钝裂银莲花花粉可育率很高,达80%左右。花粉直径为28.75~33.75 m 。(2)TTC浓度影响花粉活力的测定,当浓度为0.5%时,所测花粉活力的效果较好。(3)I2-I法的染色效果不理想,染色率仅为8.33%~22.2%,且3种花色花粉活力测定数据间差异不显著。(4)3种花色钝裂银莲花花粉活力有差异,黄色最高,浅黄色次之,白色最低。TTC染色法是简单快速测定钝裂银莲花花粉活力的最适方法。一天内花粉活力在12:00~14:00最大。

关键词: 钝裂银莲花, 测定方法, 可育性, 花粉, 活力

Abstract: The pollen viability of Anemone obtusiloba with three-olor flowers (yellow, pale yellow and white) was detected with four methods: TTC dyeing, I2-I dyeing, inorganic acid test, sucrose-ased germination, the determination effects of different methods were compared, and the dynamic change of pollen viability within a day was understood. The results show that: (1) The fertile rate of pollen of A.obtusiloba is high, about 80%. Pollens diameter are 28.75-33.75 m. (2) The concentration of TTC can influence the determination of pollen vitality, when the concentration was 0.5%, it was better than the others. (3) I2-I dyeing is not ideal, dyeing rate is only 8.33%-22.2%, and there is no significant difference within three-olor flowers. (4) The pollen vitality of A.obtusiloba with three-olor flowers have differences, with yellow the highest, followed by light yellow, white the minimum. TTC dyeing is a simple and rapid determination method of A.obtusiloba. Within a day, pollen vitality is maximum from 12:00-14:00 pm.

Key words: Anemone obtusiloba, detection methods, fertility, pollen, viability

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