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植物研究 ›› 2013, Vol. 33 ›› Issue (5): 576-581.doi: 10.7525/j.issn.1673-5102.2013.05.013

• 论文 • 上一篇    下一篇

陆地棉双隐性核雄性不育系ms5ms6花药的超微结构观察

豆丽萍1,2;唐灿明1*   

  1. 1.南京农业大学农学院作物遗传与种质创新国家重点实验室,南京 210095;2.甘肃省庆阳市农业科学研究院,庆阳 745000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-09-20 发布日期:2013-09-20
  • 通讯作者: 唐灿明
  • 基金资助:
     

Ultrastructure of Anther on Double Recessive Nuclear Male-terile Line ms5ms6 of Gossypium hirsutum L.

DOU Li-Ping;TANG Can-Ming*   

  1. 1.National Key Laboratory of Crop Genetics & Germplasm Enhancement,Agronomy College,Nanjing Agricultural University,Nanjing 210095;2.Qingyang Agricultural Scientific Research Institute,Qingyang 745000
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-09-20 Published:2013-09-20
  • Contact: TANG Can-Ming
  • Supported by:
     

摘要: 陆地棉双隐性核雄性不育系ms5ms6已有较大的应用规模,但雄蕊败育的结构基础尚不明确。以核雄性不育系ms5ms6为材料,利用透射电镜对四分体时期和刚长出小刺突的小孢子时期的花药进行超微结构观察。发现在小孢子时期,败育花药小孢子只有外壁内层,可育小孢子此时已具有外壁外层和外壁内层了。在整个不育花药的发育过程中,不管是在小孢子还是绒毡层细胞中,内质网都异常,脂肪的积累少,这可能是导致小孢子败育最重要的原因。

关键词: 陆地棉, 超微结构, 花药, 核雄性不育系, 绒毡层

Abstract: The double recessive nuclear male-terile line ms5ms6 of upland cotton (Gossypium hirsutum L.) has been widely used in a large-cale. However, the structural basis of anther abortion in cytology of this material is still unknown. Transmission electron microscopy was used to observe nuclear male-terile line ms5ms6 in stages of tetrads and microspores on which there are small spinescent protuberances. The results show that the male-terile microspores have only endexine during the stage of microspore, however, the fertile microspores have sexine and endexine of cell walls. In the microspores and tapetum cells, the abnormal endoplasmic reticulum and no fat accumulation were found during male sterile anther development, which might be the most important reason of microspores abortion.

Key words: upland cotton, ultrastructure, anther, nuclear male sterile line, tapetum

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