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    20 July 2012, Volume 32 Issue 4
    Two New Species of Polygonum from China
    LI An-Jen;BAO Bo-Jian
    2012, 32(4):  385-388.  doi:10.7525/j.issn.1673-5102.2012.04.001
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    Polygonum shuense and P.chayuum, two new species of Polygonum from China, are described. P.shuense is similar to P.subscapsum, but differs by the radical leaves crenate at margins, and altenuate and decurrent at base, flowers white, styles 3 and free, and achenes long ovate and opaque. P.chayuum is distributed in Xizang, with stem unbranched, leaves on the upper part of stem, and only ochreas on the lower part of the stem. Such characters are unique in Polyonum and differ apparently from those of other species.
    Sedum fanjingshanensis C.D.Yang et X.Y.Wang——A New Species of Sedum L.
    YANG Chuan-Dong;WANG Xiao-Yu;GOU Guang-Qian*
    2012, 32(4):  389-391.  doi:10.7525/j.issn.1673-5102.2012.04.002
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    Sedum fanjingshanensis C.D.Yang et X.Y.Wang, a new species of the Sedum from Guizhou, China, is described and illustrated. The new species is related to S.correpta Fröderström, but differs in the 10 stamens in 2 rings. It is also similar to S.filipes Hemsley, but differs in having simple flower terminal.
    Anatomical Characteristics of Capparis spinosa L. in Tibet
    LIU Jing;MA Miao*
    2012, 32(4):  392-396.  doi:10.7525/j.issn.1673-5102.2012.04.003
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    To further explore the Qinghai-Tibet Plateau plant distinctive physiology structure and its environmental adaptability, the anatomical structures of Capparis spinosa L. from 4 000 m altitude of Zhada in Tibet were studied by the methods of paraffin section. The results showed that the anatomical structure was highly adapted to its environment of mountain desert grassland: periderm of secondary root is developed, the cork is thicker, there is a larger proportion of secondary xylem; There are epidermal hairs and obvious cuticle on the epidermis of stems, and also developed collenchyma tissue, pith is narrow in stems; The leaf is isobilateral with multilayer palisade tissues. Dense stomata exists and significant cuticles are existed in epidermis; Corolla is larger with white petals, which is suitable for insect pollination; Parietal placentation with much ovules in each locule, extine is thicker which can resistant to corrosion, acid and alkaline. All of the characteristics of C.spinosa L. described above result from Qinghai-Tibet Plateau stresses of extremely ecological conditions, such as high irradiance, cold temperature, low air pressure in the Qinghai-Tibet Plateau, as well as the adaptations of the C.spinosa L. to the Qinghai-Tibet Plateau environments.
    Effect of Light on Anthocyanin Biosynthesis and Metabolism of Brassica oleracea L. and Analysis on Expression Pattern of Anthocyanin Structural Genes
    LI Ya-Li;HU Zong-Li;ZHANG Bin;ZHU Ming-Ku;CHEN Guo-Ping*
    2012, 32(4):  397-401.  doi:10.7525/j.issn.1673-5102.2012.04.004
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    In order to study the influence of light on anthocyanin synthesis and metabolism of purple cabbage and the mechanism of regulation in purple cabbage, “Early Red” purple cabbage was used as the test material, and cabbage “Feng Yuan 913” (green cabbage) was used as the control. Seedlings were planted under light or dark condition for one week, and then the anthocyanin content was determined by using the pH differential spectrum method at pH 1.0 and pH 4.5. Subsequently, semi-quantitative RT-PCR was performed to analyze the expression pattern of structural genes involved in anthocyanin biosynthesis. The results showed that no significant difference was observed in expression level of structural genes involved in anthocyanin biosynthesis in purple cabbage after treated by light or dark except for PAL and UFGT. In addition, purple cabbage seedlings were still colored obviously even grew in the dark, but the young seedlings of green cabbage turned up albefaction completely. The expression levels of downstream structural genes in anthocyanin synthesis pathway were significantly increased in purple cabbage compared with those of green cabbage. At the same time, young seedlings were colored deeply, indicating that mass anthocyanin in purple cabbage was correlated with high expression of these genes.
    Promotion of Photosynthesis of Transgenic Rice Plant with Overexpressing C4 pepc from Maize under Low Concentration NO
    CHEN Ping-Bo;LI Xia*
    2012, 32(4):  402-409.  doi:10.7525/j.issn.1673-5102.2012.04.005
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    In order to reveal the relationship between physiological difference and high photosynthetic efficiency in C3 plant with high expression C4 pepc, the transgenic rice with high expression of maize C4 pepc(PC) and the untransformed rice plant Kitaake (WT) were used as the material plants. The upsecond leaves of the rice plants were used to treat by root system during the booting stage. After the treatments of the different concentrations of NO donor, NO synthesis inhibitors and related signal related reagents as well as in the dark for 12 h, the effects of NO on the net photosynthetic rate (Pn), stomatal conductance (Gs) and intercellular CO2 concentration (Ci) of the test material leaves were measured. The results showed that: Pn of WT and PC increased by 20.8% and 10.7%, respectively under the treatments of 200 μmol·L-1 SNP(Sodium nitroprusside) and 1 mmol·L-1 L-Arg (L-Arginine), which was significantly different(p<0.05); However, with increasing the concentrations of SNP and L-Arg, the Pn, Gs and Ci of two rice plants were gradually suppressed. Compared with PC, the Pn of WT was significantly inhibited (p<0.05), but the changes of Gs and Ci were opposite; When treated by 200 μmol·L-1 SNP and 1 mmol·L-1 L-Arg as well as SA (salicylic acid) for the test materials, the changes of Pn, Gs and Ci were similar with those under high concentrations of NO donors treatment; Under united 6 mmol·L-1 EGTA of Ca2+ chelator treatment, compared with the PC, the Pn of WT was significantly inhibited at the level (p<0.01), that Ci was opposite (p<0.05). Correlation analysis showed that: The correlationship between the Pn and the Gs in PC was less than that of WT, the determination coefficients were 0.654 9 and 0.773 5; However, The correlationship between the Pn and the Ci in PC was larger than that of WT, the determination coefficients were 0.466 5 and 0.419 6, which implied that there might be different way of the regulation of photosynthesis between PC and WT, especially at lower concentrations of NO in PC, NO might participate in the process of the stomatal movement with high concentration of free Ca2+. It can still maintain the stability of Pn in PC under the conditions in stomatal closure.
    Gas Exchange and Leaf Structure of Saxifraga stolonifera Curt. under Different Temperatures
    HE An-Na;;LIN Wen-Qiang;YAO Yi;TAN Xiao-Li
    2012, 32(4):  410-414.  doi:10.7525/j.issn.1673-5102.2012.04.006
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    The potted Saxifraga stolonifera Curt. was treated in the artificial climate chamber. The photosynthetic characteristics, chlorophyll content, antioxidant enzyme systems, leaf structure and other forms of physiological indicators were measured under different temperature conditions. The results showed that, net photosynthetic rate and stomatal conductance of S.stolonifera decreased rapidly under temperature treatment, content of chlorophyll was the least, SOD, CAT activities were the minimum, MDA content was the highest, palisade tissue arranged more closely, and had a good recovery in a short time. The decreased speed of net photosynthetic rate of high temperature treatment was less than the low temperature treatment, but had a significant increase in leaf spongy tissue, and recovery was slow.
    Influence of Light quality and Quantity on Germination and Seedling Status of Invasive Species Eupatorium adenophorum
    JIANG Yong;WANG Wen-Jie*;LI Yan-Hong;BAO Shong-Lian
    2012, 32(4):  415-419.  doi:10.7525/j.issn.1673-5102.2012.04.007
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    For understanding the features of light-demanding seed germination of Eupatorium adenophorum, the influences of light qualities (different colors, red/far-infrared light) and light intensity on seedling sprouting of this weed were studied in this paper. Lights of different colors have significantly influence on the parameters of seedling sprouting, i.e., yellow light, orange light and red light, which have longer wavelength, were more suitable for facilitating seed germination rate (74.3%-83.3%), while the shorter wavelength lights (<570 nm, violet, blue and green lights) had significant lower seed germination rates (62.3%-66.7%)(p<0.05). Light intensity had markedly more regularity on seed germination and seedling status than the color of the light. Under the condition of darkness, germination rate was 22%. Along with the light enhancement both in transmittance and lux, exponential increases in seed germination (r2=0.96) and root length (r2=0.98) were observed, while height of seedlings exponentially decreased (r2=0.99); a linear increase in fresh mass was simultaneously observed (r2=0.70). Appropriate radiation of red light (630 nm) and far-infrared light (730 nm) can break and cause dormancy. The exposure amount of red light linearly related with germination increase with reference to dark control (r2=0.98), while the amount of far-infrared light was linearly correlated with germination decrease with reference to control (r2=0.92). This indicates that light-demanding seed germination of E.adenophorum is a phytochromes-induced process. E.adenophorum generally runs rampant in barren and disturbed lands and light sensitive germination should be partially responsible for that. The ecological control of this weed should consider light regulation, in particular the light quality (such as afforestation decreases red light/far-infrared ratio) to decrease seed germination.
    Isolation and Analysis of Differential Expressed ESTs from Solms-laubachia eurycarpa(Maxim.) Botsch. between Day and Night
    LIU De-Tuan;LI Wan-Sha;HU Xiang-Yang*
    2012, 32(4):  420-424.  doi:10.7525/j.issn.1673-5102.2012.04.008
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    The wide-distribution species Solms-laubachia eurycarpa on the scree on Baima snow mountain was used as the experimental material, and the suppression subtractive hybridization (SSH) based on magnetic beads technology was used to isolate the differential genes between day and night. After randomly sequencing 136 differential ESTs, these ESTs were further analyzed by functional annotation by Blast2go. Our results show that most ESTs are related to cellular homeostasis and stress response, followed by material and energy metabolism, and signal transduction. The process of S-L.eurycarpa responsive to daytime environment is very complicated, which lays a good foundation for further studying alpine plants adaptation to harsh environments of scree.
    Relationships between Leaf Nitrogen Content and Photosynthetic Characteristics in Different Plant Functional Types
    MIAO Yan-Ming;LV Jin-Zhi;BI Run-Cheng*
    2012, 32(4):  425-429.  doi:10.7525/j.issn.1673-5102.2012.04.009
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    Functional Analysis of a Zinc-finger-like Gene from Nicotiana tabacum
    QU Guan-Zheng;ZHENG Tang-Chun;MA Lin;SHAO Long-Ting;CAO Feng-Juan;LI Kai-Long*
    2012, 32(4):  430-436.  doi:10.7525/j.issn.1673-5102.2012.04.010
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    In the present study, a Zinc-finger-like cDNA (NtZFL) was cloned from Nicotiana tabacum, the gene open reading frame (ORF) was 321 bp in length and encoded a predicated polypeptide of 106 amino acids. Expression of NtZFL in tobacco plant in response to biotic and abiotic stress was studied by QRT-PCR, the results showed that NtZFL was responsive to the treatments of MV, H2O2, ABA and cold. Northern blot analysis showed that NtZFL gene was expressed in different tissues of tobacco plant, achieving maximum in the young leaves and flowers. Subcellular localization analysis showed that the NtZFL protein was localized in the cell wall. GUS activity driven by NtZFL promoter was expressed in the whole of the young plant, but strongly detected in roots and leaf veins.
    Cloning and Analysis of Caffeic Acid O-methyltransferase Gene(SmCOMT1) from Salvia miltiorrhiza Bge.
    ONG Yin;WANG Dong-Hao;WU Jin-Bin;ZHOU Lu;WANG Guo-Dong;WANG Zhe-Zhi*
    2012, 32(4):  437-443.  doi:10.7525/j.issn.1673-5102.2012.04.011
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    According to the sequencing result of caffeic acid O-methyltransferase from Salvia miltiorrhiza transcriptome database analysis, its specific primers were designed. By RT-PCR method, a novel COMT gene was isolated from S.miltiorrhiza, and named as SmCOMT1(Genebank accession number: JF693491). SmCOMT1, with full-length cDNA of 1 158 bp, includes an open reading frame of 1 095 bp which encodes a 364 amino acids polypeptide. Furthermore, a length of 2 275 bp sequence was also cloned by PCR from genomic DNA of S.miltiorrhiza. The genomic DNA of SmCOMT1, aligned with cDNA, contains four exons and three introns in the encoding region. The results of amino acid sequence analysis shows that deduced amino acid polypeptide contains all the conserved element of COMT family and it is highly homologous to COMT proteins from the same family of Ocimum basilicum with 89% identity. Phylogenetic tree analysis also indicates that SmCOMT1 is more related to the genetic relationship of COMT in dicotyledonous plants. Quantitative RT-PCR analysis revealed that SmCOMT1 was expressed in different organs, and was highly expressed in stem, and could be induced by methyl jasmonate (MeJA) and pathogen. These results showed that SmCOMT1 might be pathogen-responsive gene in plant defenses.
    Cloning and Sequence Analysis of 1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate Synthase Gene cDNA from Eucommia ulmoides
    LIU Pan-Feng;DU Hong-Yan*;DU Lan-Ying;WU YUN Ta-Na;HUANG Hai-Yan
    2012, 32(4):  444-451.  doi:10.7525/j.issn.1673-5102.2012.04.012
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    1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase (HDS) catalyses 2C-methyl-D-erythritol-2,4-cyclodiphosphate into 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate in the penultimate step of 2C-methyl-D-erythritol 4-phosphate (MEP) pathway, which is an alternative plant terpenoids biosynthetic route that has been recently discovered. Homologous HDS cDNA (EuHDS) was isolated from leaves of Eucommia ulmoides by the methods of reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technique. The full-length cDNA of EuHDS was 2 786 bp and encoded 743 amino acids with a predicted molecular mass of 82.25 kD, and a theoretical isoelectric point of 5.89. Two conserved motifs and three absolutely conserved cysteines (residues 644, 647 and 678) were discovered in the deduced coding sequence. Phylogenetic analysis revealed that EuDXR was more similar to Vitis vinifera(evolutionary distance, 0.049) than that of other species, followed by Solanum lycopersicum(0.052) and Hevea brasiliensis(0.052).
    Analysis of Liriope spicata and Its Related Species Based on DNA Barcoding Technique
    XU Jin-Bang;ZHANG Jun-Yi*
    2012, 32(4):  452-457.  doi:10.7525/j.issn.1673-5102.2012.04.013
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    To discuss the potential of cpDNA trnL-F and psbA-trnH as DNA barcode of Liriope spicata(Thunberg) Loureiro and its related species, cpDNA trnL-F and psbA-trnH sequences were analysed. These two regions were respectively amplified by PCR and then purified and sequenced successfully. The results showed that the lengths of trnL-F sequences in 18 plant samples belonging to 9 species of liriopogons varied from 355 to 356 bp. There was no difference in the trnL-F of various species of liriope, but some specifically variable sites were found in this fragment, which led to the identification between Ophiopogon japonicus(Thunb.) Ker-Gawl and Ophiopogon bodinieri Levl. The length of psbA-trnH region was 543-544 bp, there were only some identification sites in O.japonicus(Thunb.) Ker-Gawl. Because of the low rate of evolution and the high conservation, these two cpDNA spacer regions could be only appropriate as a DNA barcode to identify liriopogons at generic level, but there is a degree of limit at lower taxonomic level.
    Monitoring of Chemical Components Changes during Tussilago farfara L. Development using NMR-based Metabolomics
    ZHI Hai-Juan;SUN Hai-Feng;ZHI Peng;LI Zhen-Yu*;QIN Xue-Mei
    2012, 32(4):  458-466.  doi:10.7525/j.issn.1673-5102.2012.04.014
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    NMR-based metabolomics were used to investigate the metabolites of Tussilago farfara L. at different growth stages. Using 1D and 2D NMR spectra, 34 polar metabolites and 6 apolar metabolites were assigned. The 1H NMR data were then subjected to principal component analysis and the results showed clear separation of all the different stages of T.farfara L. Samples of October, November and December were in close proximity on the plot, indicating similar metabolome of these periods; samples from September (early development) and March (after flowering) were far away, showing significant chemical differences. With bud development, sucrose content gradually decreased and reached the lowest after flowering; tussilagone level gradually increased to the highest after flowering. The result of this study was in accordance with the traditional use of this herbal medicine. Metabolites of the early and flowering stages were quite different from those of the traditional harvest time, suggesting that they could not be used as traditional medicine. In addition, antitussive and expectorant effects were the results of synergistic effects of a variety of ingredients.
    ISSR Analysis on the Population Genetic Structure of Camellia japonica of Japanese Islands
    LIN Li;NI Sui;CHEN Yue;LI Ji-Yuan*;FAN Zheng-Qi;YING Zhen
    2012, 32(4):  467-472.  doi:10.7525/j.issn.1673-5102.2012.04.015
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    Camellia japonica is an important horticultural species widely distributed in East-Asia. Five populations of C.japonica were analyzed by ISSR markers to detect the genetic structure. Twenty primers produced scorable markers were applied. The percentage of polymorphic loci, Nei’s gene diversity and Shannon’s Information Index were 70.29%, 0.281 9 and 0.409 5, indicating C.japonica had a higher level of genetic diversity than other insular plants. The coefficient gene differentiation (Gst) was 0.205 7, the effect of geographical isolation had great influences and significant correlation was found between genetic distance and geographic distance (r=0.821 7, P<0.05). UPGMA analysis revealed that populations in the same island had a close relationship. Taking examples from the experience of Japan, management strategies were proposed that natural population should be protected in situ from human disturbance to facilitate its natural generation. Constructing germplasm resources and cultivating plants mutually collected from different populations are necessary to enhance the gene flow.
    Structures and Dynamics of Castanopsis sclerophylla Population in Tianmu Mountain of Zhejiang Province
    YUE Hua-Feng;JING Zhen-Hua;SHAO Wen-Hao;JIANG Jing-Min*;XIA Xin;Huang Lin
    2012, 32(4):  473-480.  doi:10.7525/j.issn.1673-5102.2012.04.016
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    The population structures and dynamics of Castanopsis sclerophylla were studied from six sampling plots in Tianmu Mountain in Zhejiang province. The results showed that all the populations of C.sclerophylla were spindle type. The age of structure of populations was declining due to lacking seedling and sapling. The overall population distribution followed a random type. Population dynamics analysis showed the spatial distribution pattern changed from aggregate type to random type during the course of population growth. One reason is that scores of seeds from populations were eaten by rodents, the other reason is that the patterns were not only closely related to the ecological and biological characteristics of each species and the relationships of competitive exclusion among them, but also to the environment. The characteristics of population reproductive mode are that the contribution rate of asexual reproduction is far greater than sexual reproduction. Maintenance and natural regeneration of C.sclerophylla populations adopted mostly sprout regeneration. To a certain extent, it affects the direction of succession. C.sclerophylla populations should restore the ecological balance through controlling rodents and transplanting seedling.
    Induction and Culture Condition of Hairy Roots of Cajanus cajan(L.) Millsp.
    YU Hai-Di;WANG Hui-Hei;FU Yu-Jie*
    2012, 32(4):  481-483.  doi:10.7525/j.issn.1673-5102.2012.04.017
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    Hairy roots of Cajanus cajan(L.) Millsp. was induced by Agrobacterium rhizogenes LBA9402 with direct inoculation. The optimum condition of induction of hairy roots in this test was as follows, the leaves of C.cajan were used as explants to be inoculated on 1/2MS solid medium, pre-culture for 2 d, the concentration of bacterium was OD=0.6-0.8, dissemination for 20 min, co-culture for 3 d, the rate of the hairy roots was 60%. PCR analysis at molecular level proved that T-DNA of Ri plasmid of A.rhizogenes 9402 has been successfully transferred into the genome of hairy roots of C.cajan.
    In vitro Culture of Endangered Medicinal Plant Sinopodophyllum hexandrum Royle
    GUO Qi;ZHANG Jun;ZHAO Xiao-Feng;YANG Tao;WANG Pei-Ya;YANG Hui;*
    2012, 32(4):  484-487.  doi:10.7525/j.issn.1673-5102.2012.04.018
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    The seeds of Sinopodophyllum hexandrum Royle were taken as materials. The effects of exogenous hormones on induction, multiplication and rooting of explants were discussed. The regeneration system of S.hexandrum has been established. The results showed that: The optimal conditions for shoot induction were 3.0 mg·L-1 6-BA+0.5 mg·L-1 GA-3. The induction rate was 85.71% with a shortening of 30-40 d. For multiplication, the optimal conditions were 3.0 mg·L-1 6-BA+0.2 mg·L-1 IAA, and the mean shoots obtained per explant was 1.63. The most proper rooting medium was: WPM+1.5 mg·L-1 IAA+0.5 mg·L-1 NAA and the rooting ratio was 60.1% after 30 d culturing.
    Sterile Propagation of Bulbs from White Egret Orchid(Habenaria radiate) Seeds
    ZHAO Xi-Heng;LI Jin-Cai*
    2012, 32(4):  488-491.  doi:10.7525/j.issn.1673-5102.2012.04.019
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    In order to establish the seed propagation method of white egret orchid, we observed the germination and seedling growth of seeds cultured under sterile condition and conducted the planting trial of bulbs from sterile propagation. The results showed that seeds without treatment are difficult to germinate in sterile culture. The germination rate of seeds after treatment of 75% ethanol for 10 s and 1% NaClO for 10 min, or treatment of 40 kHz ultrasonic for 2.5 min and 1% NaClO for 10 min is higher than 58% in 1/2MS medium; addition of 1 mg·L-1 GA-3 in 1/2MS medium can promote leaf growth, and addition of 1 mg·L-1 IAA can promote root growth and bulb formation, the bulb formation rate of seedlings reached 55%; sterile breeding bulbs were stored for spring potting, they have seedling formation rate of 72%.
    Litter Decomposition and Nutrient Dynamic of Betula platyphylla Secondary Forest with Different Stand Ages in Xiaoxing’an Mountains
    HOU Ling-Ling;SUN Tao;MAO Zi-Jun*;LV Hai-Liang;ZHAO Juan;SONG Yuan
    2012, 32(4):  492-496.  doi:10.7525/j.issn.1673-5102.2012.04.020
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    Taking three ages Betula platyphylla secondary forests (20a, 36a, 82a) in Xiaoxing’an Mountains, China as test objects, this paper studied their remaining rates and nutrient dynamics from May 2009 to November 2010 by using decomposition bag method. The results of two year decomposition showed that the different ages of B.platyphylla secondary forests had a fast decomposing stage (0-150 d) and a slow decomposing stage (150-540 d). For all test litters, their remaining rate had an exponential relationship with time. The annual decomposition constant (k) ranged from 0.584 to 0.657, and the times for decomposing 50% and 95% were 1.06-1.19 years and 4.56-5.13 years, respectively. The decreasing order of litter decomposition rate of these three ages of the B.platyphylla secondary forests was 82a>36a>20a. During decomposition, the elements carbon and potassium in litter released continuously, and the concentrations of nitrogen and phosphorus increased first and then decreased, N concentration of three different ages forests was in enriched mode and P concentration was in released mode in the later period.
    Ultrasound-assisted Extraction of 2″-O-galloylhyperin from Pyrola calliantha H.Andr.
    YAO Xiao-Hui;DUAN Xi-Hua;*;DUAN Ming-Hui;ZHANG Dong-Yang;FU Yu-Jie;
    2012, 32(4):  497-500.  doi:10.7525/j.issn.1673-5102.2012.04.021
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    Based on the preliminary experiments, a three-variable, three-level Central Composite Design (CCD) was performed to study the ultrasound-assisted extraction of 2″-O-galloylhyperin from Pyrola calliantha H.Andr.. The optimum parameters were as follows: extraction time 45 min, ultrasound power 40 kHz, three extraction cycles, extraction temperature 60℃, liquid/solid ratio 33∶1, ethanol concentration 50%. Under the optimal conditions, the average extraction yields of 2″-O-galloylhyperin was 5.024 mg·g-1.
    Research Progresses on Plant Sucrose Transporters and Functional Regulation
    WANG Li-Fen;ZHANG Hu-Ping;ZHANG Shao-Ling*
    2012, 32(4):  501-507.  doi:10.7525/j.issn.1673-5102.2012.04.022
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    Sucrose transporters play a crucial role in sucrose transmembrane transportation during distribution of sucrose throughout the plant source-sink. This paper mainly provided a brief overview of research progresses on sucrose transporters groups, celluar localization and functional regulation in high plants.
    Progress of Haploid Production in Compositae
    ZHANG Jie;GAO Yi-Ke*
    2012, 32(4):  508-512.  doi:10.7525/j.issn.1673-5102.2012.04.023
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    Haploid induction is an important method for obtaining homozygous lines in Compositae. Haploids have been induced in 13 Compoditae species, and 9 species was successfully obtained. The pathways to obtain haploid plants in Compositae include anther culture, isolated microspore culture, in vitro gynogenesis culture, intergeneric hybridization and induction of haploid by irradiated pollen. The factors affecting in vitro induction and regeneration of haploids including developmental period of the explant, pretreatment, medium and its component, and culture condition were discussed in the paper. The methods of haploid induction in Compositae were compared. This review also proposed some problems in the Compositae haploid research, and provided reasonable suggestions.