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Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (4): 488-491.doi: 10.7525/j.issn.1673-5102.2012.04.019

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Sterile Propagation of Bulbs from White Egret Orchid(Habenaria radiate) Seeds

ZHAO Xi-Heng;LI Jin-Cai*   

  1. 1.School of Agriculture and Bioengineering,Tianjin University,Tianjin 300072;2.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-20 Published:2012-07-20
  • Contact: LI Jin-Cai
  • Supported by:

Abstract: In order to establish the seed propagation method of white egret orchid, we observed the germination and seedling growth of seeds cultured under sterile condition and conducted the planting trial of bulbs from sterile propagation. The results showed that seeds without treatment are difficult to germinate in sterile culture. The germination rate of seeds after treatment of 75% ethanol for 10 s and 1% NaClO for 10 min, or treatment of 40 kHz ultrasonic for 2.5 min and 1% NaClO for 10 min is higher than 58% in 1/2MS medium; addition of 1 mg·L-1 GA-3 in 1/2MS medium can promote leaf growth, and addition of 1 mg·L-1 IAA can promote root growth and bulb formation, the bulb formation rate of seedlings reached 55%; sterile breeding bulbs were stored for spring potting, they have seedling formation rate of 72%.

Key words: white egret orchid, seed, ethanol, ultrasonic, sterile propagation, bulb

CLC Number: