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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (6): 917-926.doi: 10.7525/j.issn.1673-5102.2019.06.015

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Cloning and Functional Analysis of BpZFP4 Promoter from Birch(Betula platyphylla)

QIN Lin-Lin, ZHANG Xi, JIANG Cheng, LI Li   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-06-09 Online:2019-11-05 Published:2019-11-16
  • Supported by:
    The National Science Foundation of China(31470676)

Abstract: The BpZFP4 plays significant roles in abiotic stress responses. To better understand the regulatory mechanisms of BpZFP4 gene expression, we cloned a 1360 bp long promoter fragment of BpZFP4 using genome walking technique. Computational analysis affirmed the existence of multiple abiotic stress responsive cis-elements as well as phytohormone, light, pathogen and wound responsive motifs. Deletion analysis of the BpZFP4 gene promoter in transgenic tobacco plants revealed the key regions and the possible cis-element regulating the BpZFP4 response to stresses such as drought, salt and ABA stress. This result may provide an important contribution for elucidating the regulation mechanisms of BpZFP4 expression in responding to abiotic stress and signalling molecule.

Key words: Betula platyphylla Suk., BpZFP4 promoter, putative cis-elements, functional analysis, abiotic stress

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