MICROSCOPIC OBSERVATION OF VACUOLES ISOLATED AND PURIFIED FROM TOBACCO LEAVES

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Bulletin of Botanical Research ›› 2003, Vol. 23 ›› Issue (1): 36-38.

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MICROSCOPIC OBSERVATION OF VACUOLES ISOLATED AND PURIFIED FROM TOBACCO LEAVES

LU Yun-Xia¹, WANG Yan-Zhi²

1. Biochemistry Department, Anhui Medical University, Hefei 230032;
2. College of Life Science, Wuhan University, Wuhan 430072

Received:2002-07-16 Online:2003-03-15 Published:2016-06-14

Abstract

Abstract: Observing vacuoles isolated and purified from tabacco leaves under optic and electronic microscopes, we obtained photographs of protoplasts, process of vacuoles released from protoplasts and vacuoles after purification. The results show that the method we used could obtain complete protoplasts and vacuoles, and the central vacuole is the largest organelle in one mature plant cell.

Key words: tobacco, protoplasts, vacuoles, photographs under optic and electronic microscopes

LU Yun-Xia, WANG Yan-Zhi. MICROSCOPIC OBSERVATION OF VACUOLES ISOLATED AND PURIFIED FROM TOBACCO LEAVES[J]. Bulletin of Botanical Research, 2003, 23(1): 36-38.

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MICROSCOPIC OBSERVATION OF VACUOLES ISOLATED AND PURIFIED FROM TOBACCO LEAVES

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