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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (3): 447-457.doi: 10.7525/j.issn.1673-5102.2020.03.017

• Research Report • Previous Articles    

Codon Usage Bias of Chalcone Synthase Gene CHS in Pinus koraiensis

LI Xiang1, FAN Zuo-Yi2, WANG Jing-Yuan2, WANG Qi2, LI Xi-Peng2, WANG De-Qiu2, KONG Ling-Yuan2, CAO Sen-Lin2, MENG Qing-Gang2, ZHAO Xi-Yang1   

  1. 1. State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040;
    2. Linjiang Forestry Bureau of Jilin Province, Linjiang 134600
  • Received:2019-08-25 Published:2020-05-29
  • Supported by:
    Genetic Diversity Evaluation Project of Pinus koraiensis Germplasm Resources(KJZXSA2019046)

Abstract: Chalcone synthase(CHS) is widely found in plants and is a key enzyme in anthocyanin synthesis pathway. It can catalyze the binding of malonyl coenzyme A and coumaryl coenzyme A to generate flavonoids. CodonW and EMBOSS were used to analyze the codon usage bias of CHS gene in Pinus koraiensis. The CHS gene of P.koraiensis were compared with those of the other 24 plants and model plant genome, which laid a foundation for the selection of appropriate expression system. The effective codon number(ENC) and GC content of CHS gene coding region were 48.92 and 0.351, respectively. The content of C+G was higher than that of A+T, and the codon preferred to end with A/T. The G+C content of most plants CHS genes is higher than that of A+T, and the codon preferred to end with C/G. By cluster analysis, the codon usage bias of P.koraiensis was similar to P.massoniana and P.densiflora pdchsx. In addition, it was found that Arabidopsis thaliana and Escherichia coli might be ideal receptor systems for CHS genetic transformation and heterogeneous expression.

Key words: Pinus koraiensis, chalcone synthase, codon usage bias, cluster analysis

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