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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (2): 196-201.doi: 10.7525/j.issn.1673-5102.2020.02.005

• Research Report • Previous Articles     Next Articles

Tissue Culture Technology of Stem Segment of Cinnamomum bodinieri var. citralifera

XIAO Zu-Fei1,2, WANG Ling-Ling1, CAO Lu-Yao1, LIAO Wen-Xuan1, JIN Zhi-Nong1, LI Feng1, Lü Xiong-Wei1, ZHANG Bei-Hong1, ZHAO Jiao1   

  1. 1. Nanchang institute of technology, Jiangxi Porvincial Engineering Reseach Center of Seed-breeding and Utilization of Camphor Trees, Nanchang 330099;
    2. Institute of biology and resources, Jiangxi academy of sciences, Nanchang 330096
  • Received:2019-07-31 Online:2020-03-05 Published:2020-03-06
  • Supported by:
    Department of education of Jiangxi province science and technology innovation project(GJJ151114);National natural science foundation project(31600497);Department of science and technology of Jiangxi province key research and development of key project(20171ACH80016,20181ACF60002);Department of science and technology of Jiangxi province key research and development of general project(20192BBFL60012)

Abstract: Cinnamomum bodinieri var. citralifera is an important tree species for landscaping, timber and oil. We studied the effects of collection season, disinfection time and hormones on tissue culture of annual branches of C.bodinieri var. citralifera. The axillary bud germination rate of stem segments from semi-lignified stem segments of C.bodinieri var. citralifera was highest with 0.1% HgCl2 disinfected for 5 min. in the Mid-May, and the browning rate and pollution rate were low. The suitable medium for stem segment germination was MS+1.0 mg·L-1 6-BA+0.05 mg·L-1 IBA, with germination ratio 60%. The suitable medium for multiplication was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 IBA, with multiplication coefficient of 4.33, plant height of 3.67 cm and ground diameter of 1.04 mm. Adventitious roots appeared at 11 d after culture of the base of tissue culture seedlings. The suitable medium for rooting was MS+1.5 mg·L-1 IBA. The rooting rate was 75.00%, the length of roots was 5.06 cm, the number of roots was 3.50, and the diameter of roots was 0.82 mm. Tissue culture seedlings with roots more than 3 had a survival rate of more than 80%.

Key words: Cinnamomum bodinieri var. citralifera, stem segment, tissue culture

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