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Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (1): 106-110.doi: 10.7525/j.issn.1673-5102.2010.01.019

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Tissue Culture and Rapid Propagation of Rare Plant in Codariocalyx motorius(Houtt.)

MAO Yan-Ping;SU Zhi-Xian*;ZOU Li-Juan;WU Qing-Gui   

  1. 1.College of Life Science,China West Normal University,Nanchong 637002;2.Sichuan Provincial Key Laboratory of Ecology and Environmental Technology,Mianyang 621000
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
  • Contact: SU Zhi-Xian
  • Supported by:
     

Abstract: The aseptic seedings of Codariocalyx motorius(Houtt.) Ohashi were the materials of our study on issue culture and rapid propagation, MS and 1/2MS were the basic mediums. Impact of different conditions on the rapid propagation of C.motorius(Houtt.) was discussed and a tissue culture system of C.motorius(Houtt.) was initially established, the best callus adventitious and bud differentiation medium was MS+6-BA 2.0 mg·L-1+NAA 0.1 mg·L-1+sugar 30 g·L-1+VC 2.0 mg·L-1, the best medium for the shoot regeneration was MS+6-BA 2.0 mg·L-1+NAA 0.05 mg·L-1, the best rooting medium was 1/2MS+NAA 0.2 mg·L-1+IAA 0.5 mg·L-1.

Key words: Codariocalyx motorius(Houtt.), multiplication of adventitious buds, tissue culture, rapid propagation

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