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Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (1): 49-52.doi: 10.7525/j.issn.1673-5102.2005.01.017

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Study on the factors of wheat mature embryos culture and plant regeneration

LI Xin-Ling1,2, QUMin3, YAN Yu-Qing1, XU Xiang-Ling1   

  1. 1. School of Life and Environmental Science, Harbin Normal University, Harbin 150080;
    2. Teaching and Research Section of Forestry Genetics and Breeding, Northeast Forestry University, Harbin 150040;
    3. Biology department of Food Engineering College, Harbin University of Commerce, Harbin 150010
  • Received:2004-11-02 Online:2005-03-15 Published:2016-06-14

Abstract: To culture wheat mature embryos from three cultivars in vitro and study the factors of wheat callus induction and plant regeneration. The result of research shows bud differentiation rate of dongnong 7742 is much higher than longmai 9814 and longmai 26. ZT with high concentration could increase the rate of bud differentiation. The bud formation could be stimulated by half amount of culture medium togetherwith NAA of low concentration. So the genotypes have great influence on wheat callus differentiation and to add certain amount of hormone could increase the rate of plant regeneration.

Key words: wheat, mature embryos, callus, plant regeneration