Expression of TaNAC1 Gene in Common Wheat and Genetic Transformation in Arabidopsis

doi:10.7525/j.issn.1673-5102.2016.06.012

Abstract

Abstract: NAC-type transcription factor is one of the plant-specific gene families, which plays key roles in regulating the development and response to environmental stress in plants. A NAC-type transcription factor, designated TaNAC1, was cloned from leaves in wheat. The encoding sequence of TaNAC1 contained five typical NAC conserved domains and belonged to ATAF sub-type NAC family. The TaNAC1 protein was located in the nucleus by transient expression in onion epidermis cell. The transcriptional expression was induced by environmental stresses such as ABA, PEG, low temperature and high salt. Phenotypic analysis in the transgenic Arabidopsis showed that over-expression of TaNAC1 caused abnormal leaves, dwarfism and fused stem in transgenic Arabidopsis. TaNAC1 maybe play important roles in regulation during the development of leaves and stems in wheat.

Key words: wheat, TaNAC1, Arabidopsis, phenotype

CLC Number:

S512.1

AI Ke-Jun, ZHU Yao, HOU He-Sheng, TONG Shao-Ming. Expression of TaNAC1 Gene in Common Wheat and Genetic Transformation in Arabidopsis[J]. Bulletin of Botanical Research, 2016, 36(6): 886-894.

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