柽柳ThDUF106基因的植物表达载体构建及其功能验证

doi:10.7525/j.issn.1673-5102.2018.02.014

植物研究 ›› 2018, Vol. 38 ›› Issue (2): 260-267.doi: 10.7525/j.issn.1673-5102.2018.02.014

柽柳ThDUF106基因的植物表达载体构建及其功能验证

葛晓兰, 刘彩霞, 张鑫鑫, 李莹, 曲冠证

东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040

收稿日期:2017-09-27 出版日期:2018-03-15 发布日期:2018-03-23
通讯作者: 曲冠证 E-mail:quguanzheng@yahoo.com
作者简介:葛晓兰(1992-),女,硕士研究生,主要从事林木遗传育种研究。
基金资助:
林木遗传育种国家重点实验室（东北林业大学）创新项目（2015A01）；国家自然科学基金项目（31370661）

Construction and Functional Verification of Plant Expression Vector of Tamarix ThDUF106 Gene

GE Xiao-Lan, LIU Cai-Xia, ZHANG Xin-Xin, LI Ying, QU Guan-Zheng

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040

Received:2017-09-27 Online:2018-03-15 Published:2018-03-23
Supported by:
State key laboratory of forest genetics and breeding(Northeast Forestry University) Innovation project(2015A01);The National Natural Science Fund(31370661)

摘要/Abstract

摘要： 柽柳中的ThDUF106基因在盐碱胁迫处理下表达量显著上调，表明ThDUF106基因可能参与了柽柳的抗逆境胁迫。为探究柽柳ThDUF106基因的功能，我们对三月龄柽柳进行盐碱、干旱及重金属等非生物胁迫下的叶、根进行荧光定量PCR检测，分析ThDUF106基因的时空表达情况，并通过生物信息学分析ThDUF106基因所编码的蛋白质，同时我们将这个基因进行植物表达载体构建并进行烟草的遗传转化。研究结果表明：转基因烟草株系及野生型的一月龄苗在非生物胁迫处理下，进行生理生化指标比较分析，结果显示，转基因烟草与非转基因烟草在各胁迫下的抗氧化状态基本相同，表明这个基因在烟草中过表达并没有改善烟草的抗氧化性。

关键词: 柽柳, ThDUF106, 遗传转化, 烟草, 非生物胁迫

Abstract: ThDUF106 gene were involved in the stress tolerance of Tamarix hispida, and the expression of ThDUF106 was up-regulated in T.hispida under salt-alkali stress. In order to study the function of ThDUF106 gene in T.hispida, the temporal and spatial expression of these gene was detected by qRT-PCR in the leaves and roots of three-month T.hispida under abiotic stress such as salinity, drought and heavy metals. The gene encoding this gene was also analyzed, and the gene was constructed and transformed into tobacco by the plant expression vector. Transgenic tobacco lines and wild-type one-month seedlings were treated, and physiological and biochemical indexes were compared and analyzed. The antioxidant status of transgenic and wild type tobacco was basically the same under various duress, indicating that the gene was not expressed in tobacco and could not improve the antioxidation of tobacco.

Key words: Tamarix hispida, ThDUF106, genetic transformation, tobacco, abiotic stress

中图分类号:

S793.5

葛晓兰, 刘彩霞, 张鑫鑫, 李莹, 曲冠证. 柽柳ThDUF106基因的植物表达载体构建及其功能验证[J]. 植物研究, 2018, 38(2): 260-267.

GE Xiao-Lan, LIU Cai-Xia, ZHANG Xin-Xin, LI Ying, QU Guan-Zheng. Construction and Functional Verification of Plant Expression Vector of Tamarix ThDUF106 Gene[J]. Bulletin of Botanical Research, 2018, 38(2): 260-267.

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柽柳ThDUF106基因的植物表达载体构建及其功能验证

Construction and Functional Verification of Plant Expression Vector of Tamarix ThDUF106 Gene

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