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植物研究 ›› 2021, Vol. 41 ›› Issue (5): 824-835.doi: 10.7525/j.issn.1673-5102.2021.05.021

• 研究报告 • 上一篇    

超声辅助提取红花中主要活性成分的工艺优化

李钰1, 杨晓1, 叶霄1, 邓洁琼1, 黄位年1, 代沙2()   

  1. 1.四川省农业科学院经济作物育种栽培研究所,成都 610300
    2.四川省攀枝花市农林科学研究院,攀枝花 617000
  • 收稿日期:2020-08-11 出版日期:2021-09-20 发布日期:2021-07-05
  • 通讯作者: 代沙 E-mail:daisweet@foxmail.com
  • 作者简介:李钰(1988—),女,助理研究员,主要从事中药材遗传育种方面的研究。
  • 基金资助:
    国家现代农业产业技术体系建设专项资金助(CARS-21);四川省“十三五”农作物及畜禽育种攻关项目(2016NYZ0036-4-1);四川省财政创新能力提升工程项目(2016GYSH003);四川省科技支撑计划项目(2014SZ0134)

Optimization on the Main Active Components Extraction Using Ultrasonic-assisted Method from Safflower

Yu LI1, Xiao YANG1, Xiao YE1, Jie-Qiong DENG1, Wei-Nian HUANG1, Sha DAI2()   

  1. 1.Industrial Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610300
    2.PanZhiHua Academy of Agricultural and Forestry Sciences,Panzhihua 617000
  • Received:2020-08-11 Online:2021-09-20 Published:2021-07-05
  • Contact: Sha DAI E-mail:daisweet@foxmail.com
  • About author:LI Yu(1987—),female,assistant researcher,mainly engaged in research on genetic breeding of Chinese medicinal materials.
  • Supported by:
    National Modern Agricultural Industry Technology System of China(CARS-21);Sichuan Province Special Projects in Crop and Livestock Breeding during the 13th Five-year Plan(2016NYZ0036-4-1);Sichuan Province Financial Innovation Ability Promotion Project(2016GYSH003);Sichuan Science and Technology Support Program(2014SZ0134)

摘要:

采用HPLC-UV测定红花提取物中黄酮类成分含量,确定评价指标,通过单因素试验设计,筛选红花活性成分提取方法;利用Box-Behnken试验设计原理,以花瓣中主要黄酮类成分为响应值,以单因素结果所选因素为自变量,建立响应面分析试验模型,优化提取工艺。红花花瓣中主要黄酮类成分为羟基红花黄色素A和山奈酚-3-O-芸香糖苷,占比85%以上;红花活性成分提取方法应采用超声提取法,响应面优化的最佳提取条件为:料液比0.1∶25 g·mL-1、超声温度70℃、超声时间45 min、溶剂为57%甲醇、超声功率177 W,在该最优条件下红花黄色素A提取率为1.74%,山奈酚-O-β-芸香糖苷提取率为1.53%。该方法羟基红花黄色素A提取率比药典中提取方法显著提高,其余黄酮类成分提取率也呈极显著增加。

关键词: 红花, 羟基红花黄色素A, 山萘酚-3-O-芸香糖苷, 提取, 响应面法

Abstract:

To extract the main components from Carthamus tinctorius L., the Box-Behnken design method and response surface methodology were used to optimize the extraction process of C. tinctorius L.; Hydroxy-safflower yellow A and kaempferol-3-O-rutinoside were the main flavonoids in C. tinctorius L. petals . The results showed that Ultrasonic extraction method were the optimal extraction technology: using 57% methanol as solvent, liquid-material ratio was 0.1∶25 g·mL-1, ultrasonic temperature was 70℃, extraction time was 45 min, ultrasound power was 177 W, Under these conditions, hydroxy-safflower yellow A extraction yield was 1.74%, kaempferol-3-O-rutinoside extraction yield was 1.53%. The safflower main ingredient extraction was recommended to use ultrasonic extraction.

Key words: Carthamus tinctorius L., Hydroxy-safflower yellow A, kaempferol-3-O-rutinoside, extraction technology, response surface methodology

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