西红花FT同源基因的表达及功能分析

doi:10.7525/j.issn.1673-5102.2022.02.007

植物研究 ›› 2022, Vol. 42 ›› Issue (2): 224-233.doi: 10.7525/j.issn.1673-5102.2022.02.007

西红花FT同源基因的表达及功能分析

王桢¹, 杨柳燕¹, 裴卫忠², 李心¹, 杨贞¹, 张永春¹()

^1.上海市农业科学院林木果树研究所，上海 201499
^2.上海市药材有限公司，上海 200003

收稿日期:2020-08-15 出版日期:2022-03-20 发布日期:2022-02-22
通讯作者: 张永春 E-mail:saasflower@163.com
作者简介:王桢（1986—），女，助理研究员，博士，主要从事花卉育种与分子生物学研究。
基金资助:
上海市科委项目(19391901000);上海市科委研发平台专项(18DZ2291400);上海市农委产业技术体系建设专项(沪农科产字（2020）第8号)

Expression and Functional Analysis of FT Homologous Genes in Saffron(Crocus sativus L.)

Zhen Wang¹, Liuyan Yang¹, Weizhong Pei², Xin Li¹, Zhen Yang¹, Yongchun Zhang¹()

^1.Forest & Fruit Tree Institute，Shanghai Academy of Agricultural Sciences，Shanghai 201499
^2.Shanghai traditional Chinese medicine Co. ，LTD，Shanghai 200003

Received:2020-08-15 Online:2022-03-20 Published:2022-02-22
Contact: Yongchun Zhang E-mail:saasflower@163.com
About author:Wang Zhen（1986—），female，research associate，Ph.D，mainly engaged in flowering breeding and molecular biology.
Supported by:
Project of Shanghai Science and Technology Commission,China(19391901000);Research and development platform of Shanghai Science and Technology Commission,China(18DZ2291400);Agriculture Research System of Shanghai,China(202008)

摘要/Abstract

摘要：

FT（FLOWERING LOCUS T）及其同源基因作为三大开花途径整合子之一，被认为是调控植物开花的重要基因。为了深入研究FT同源基因的功能以及西红花（Crocus sativus L.）开花的分子机理，对已报道的3个西红花FT同源基因（CsatFT1、CsatFT2和CsatFT3）进行分离及分析。gDNA包含长度分别为835、1 642和1 132 bp的完整开放阅读框（ORF），均具有4个外显子和3个内含子；cDNA包含长度分别为528、525和540 bp的ORF，分别编码175、174和179个氨基酸；系统进化分析表明，CsatFT1、CsatFT2、CsatFT3分别和同为单子叶植物的水仙（Narcissus chinensis）NtFT、麝香百合（Lilium longiflorum）LlFT和洋葱（Allium cepa）AcFT1表现出较近的遗传距离。qRT-PCR分析结果显示，小球茎膨大阶段前期，CsatFT1、CsatFT2、CsatFT3在叶片中表达水平最高，侧根中次之，子球茎、主根中极低几乎检测不到；小球茎膨大阶段后期，CsatFT1、CsatFT2、CsatFT3都在子球茎中表达水平较高，在顶芽中几乎检测不到；室内储藏开花阶段，CsatFT1、CsatFT2、CsatFT3在柱头中表达水平最高，叶中次之，花瓣和花药中较低几乎检测不到。通过观测转基因烟草（Nicotiana tabacum）和转基因拟南芥（Arabidopsis thaliana）植株表型发现，CsatFT1，CsatFT2和CsatFT3均具有促进植物提早开花的功能。

关键词: 西红花, 成花诱导, FT同源基因, 基因功能

Abstract:

As one of the three floral pathway integrators， FT（FLOWERING LOCUS T） and its homologous genes were considered to be important genes which related to flowering. In order to clarify the function of FT homologous genes and flowering mechanism of saffron（Crocus sativus L.）， the three reported FT homologous genes， named CsatFT1， CsatFT2 and CsatFT3， were isolated and analyzed. The gDNA contained the ORF with length of 835， 1 642 and 1 132 bp， respectively， and they all had 4 exons and 3 introns. cDNA sequences contained the length of 528， 525 and 540 bp ORF， which encoded 175， 174 and 179 amino acids， respectively. The phylogenetic analysis indicated that CsatFT1， CsatFT2 and CsatFT3 showed relatively close genetic distance with the monocotyledon Narcissus tazetta NtFT， Lilium longiflorum LlFT and Allium cepa AcFT1， respectively. qRT-PCR results indicated that the expression levels of CsatFT1， CsatFT2 and CsatFT3 were the highest in the leaves， followed by the lateral roots， and almost no expressed in daughter corms and primary roots at the early stage of replacement corm enlargement. At the late stage of replacement corm enlargement， the expression levels of CsatFT1， CsatFT2 and CsatFT3 were all high in daughter corms and were almost not detected in terminal buds. During indoor storage stage， the expression levels of CsatFT1， CsatFT2 and CsatFT3 were high in the capital， followed by the leaves， and almost undetectable in the petals and anthers. From the phenotypes of transgenic tobacco and transgenic Arabidopsis plants， the results showed that CsatFT1， CsatFT2 and CsatFT3 could promote the early flowering of plants.

Key words: Saffron（Crocus sativus L.）, floral induction, FT homologous gene, gene function

中图分类号:

Q785

王桢, 杨柳燕, 裴卫忠, 李心, 杨贞, 张永春. 西红花FT同源基因的表达及功能分析[J]. 植物研究, 2022, 42(2): 224-233.

Zhen Wang, Liuyan Yang, Weizhong Pei, Xin Li, Zhen Yang, Yongchun Zhang. Expression and Functional Analysis of FT Homologous Genes in Saffron(Crocus sativus L.)[J]. Bulletin of Botanical Research, 2022, 42(2): 224-233.

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名称 Name	正向引物 Forward primer（5′—3′）	反向引物 Reverse primer（5′—3′）
CsatGAPDH	ATAGCTTATTATTAGACGGA	CTCAAGGGAATCATGGGCT
CsatFT1	AATGAGTAGGGATTCGCTGGTG	GCGGGTTACTTGGATTTGGA
CsatFT2	AGAGTCCACGACCGTCGCTC	TAAATCCTTCTCCCACCGCA
CsatFT3	GTTTATTACAACTGCCAGAGGGAG	AAGGCTTTGGAAGAATGATTTCAAG

名称 Name	正向引物 Forward primer（5′—3′）	反向引物 Reverse primer（5′—3′）	长度 Length /bp
NtEF1α	GAGGCACTTCCTGGTGACAAT	GGGCTCCTTCTCAATCTCCTTAC	273
AtEF1α	CAAGATGGATGCCACTACCC	AGTGGGAGACGAAGGGGCT	255
CsatFT1	ATTCGCTGGTGCTCGGTAG	CCTCAGATGCGGGTTACTTG	230
CsatFT2	GGTCGGAAATGGATGTGAG	CTCTCGTAGCACACAATCTCCTG	222
CsatFT3	GAGATTGGTGGCTGTGACTAT	TGCTGGAACAGGATAAACACG	218

	数量 No.	开花时主杆叶片数量 No. leaves on main stem at flowering	现蕾时植株高度 Plant height at first flower bud /cm	从移栽到现蕾的时间 Time between transformed plantlet regeneration and first flower bud /d
Wt	6	13.70±1.34a	29.10±1.86b	68.10±1.86a
35S：：CsatFT1	25	13.20±1.40a	32.00±3.20a	59.90±3.21c
35S：：CsatFT2	20	13.10±1.20a	32.30±2.91a	61.80±3.79b
35S：：CsatFT3	25	12.50±1.35a	28.90±2.85b	59.50±2.37c

西红花FT同源基因的表达及功能分析

Expression and Functional Analysis of FT Homologous Genes in Saffron(Crocus sativus L.)

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