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植物研究 ›› 2020, Vol. 40 ›› Issue (4): 635-640.doi: 10.7525/j.issn.1673-5102.2020.04.019

• 研究简报 • 上一篇    

响应面优化酶解—微波辅助提取桑叶多糖的工艺研究

朱亚伟1,2, 王立涛3, 安娟艳3, 吕慕洁3, 孙建航4, 郭娜1,2, 付玉杰1,2   

  1. 1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
    2. 东北林业大学化学化工与资源利用学院, 哈尔滨 150040;
    3. 北京林业大学林学院, 北京 100083;
    4. 哈尔滨医科大学附属第一医院神经内科, 哈尔滨 150000
  • 收稿日期:2020-03-06 发布日期:2020-06-12
  • 通讯作者: 付玉杰,E-mail:yujie-fu@163.com E-mail:yujie-fu@163.com
  • 作者简介:朱亚伟(1991-),男,硕士研究生,主要从事天然产物的靶向印迹材料的制备与应用研究。
  • 基金资助:
    “十三五”国家重点研发计划项目(2016YDF0600805);黑龙江省应用技术研究与开发项目(GX18B003);111创新引智项目(B20088)

Response Surface Optimized Enzymatic Hydrolysis-microwave Assisted Extraction Process of Mulberry Leaf Polysaccharide

ZHU Ya-Wei1,2, WANG Li-Tao3, AN Juan-Yan3, Lü Mu-Jie3, SUN Jian-Hang4, GUO Na1,2, FU Yu-Jie1,2   

  1. 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
    2. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040;
    3. College of Forestry, Beijing Forestry University, Beijing 100083;
    4. Department of Neurology, The First Hospital Affiliated of Harbin Medical University, Harbin 150000
  • Received:2020-03-06 Published:2020-06-12
  • Supported by:
    National Key R&D Program of China(2016YDF0600805);Applied Technology Research and Development Project of Heilongjiang Province(GX18B003);111 Innovation and Intelligence Introduction Project(B20088)

摘要: 采用响应面优化酶解——微波辅助法从桑叶中提取桑叶多糖的提取工艺。在单因素试验的基础上通过采用Box-Behnken方法,研究液固比、提取时间、提取温度对桑叶多糖提取率的影响。结果显示,拟合方程显著,最终确定桑叶多糖的最优提取条件为:酶含量2%、酶解pH6、酶解温度50℃、酶解时间20 min、液固比15 mL·g-1、提取时间13 min、提取温度76℃,该条件下桑叶多糖的实际提取率为15.23%,与理论模拟值15.12%接近,建立的模型真实可靠。该方法用于提取桑叶中的多糖类成分,工艺简单、成本低,具有有较高的应用价值。

关键词: 桑叶, 桑叶多糖, 酶解——微波辅助提取, 响应面分析, 提取工艺

Abstract: The response surface analysis was used to optimize the process of extracting polysaccharide from mulberry leaves by enzymatic hydrolysis-microwave assisted method.Based on the single factor test, the Box-Behnken method was used to study the effects of liquid-solid ratio, extraction time and extraction temperature on the extraction rate of mulberry leaf polysaccharides. The fitting equation was significant, and the optimal extraction conditions of mulberry leaf polysaccharides were finally determined as follows:enzyme content 2%, enzymolysis pH6, enzymolysis temperature 50℃, enzymolysis time 20 min, liquid-solid ratio 15 mL·g-1, extraction time 13 min, and extraction temperature 76℃.Under these conditions, the actual extraction rate of mulberry leaf polysaccharide was 15.23%, which was closed to the theoretical simulation value of 15.12%. Therefore, the established model was true and reliable. The method can be used for extracting polysaccharide components in mulberry leaves with the advantages of simple process, low cost and high application value.

Key words: Morus alba L., mulberry leaves polysaccharides, enzymatic hydrolysis-microwave assisted extraction, response surface methodology, extraction process

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