巴西橡胶树HbMYB62转录因子基因的克隆和表达分析

doi:10.7525/j.issn.1673-5102.2017.06.020

植物研究 ›› 2017, Vol. 37 ›› Issue (6): 953-960.doi: 10.7525/j.issn.1673-5102.2017.06.020

巴西橡胶树HbMYB62转录因子基因的克隆和表达分析

陆燕茜¹, 张冬¹, 王立丰², 王纪坤²

1. 海南省热带生物资源可持续利用重点实验室, 海南大学热带农林学院, 海口 570228;
2. 中国热带农业科学院橡胶研究所, 农业部儋州热带作物科学观测试验站, 儋州 571737

收稿日期:2017-05-03 出版日期:2017-11-15 发布日期:2017-11-25
通讯作者: 王立丰 E-mail:lfwang@catas.cn
作者简介:陆燕茜(1992-),女,硕士研究生,主要从事分子植物病理学研究。
基金资助:
国家自然科学基金（31570591，31270643）

Cloning and Expressional Analysis of HbMYB62 under Multi-stimulation in Hevea brasiliensis Muell. Arg.

LU Yan-Xi¹, ZHANG Dong¹, WANG Li-Feng², WANG Ji-Kun²

1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228;
2. Danzhou Investigation and Experiment Station of Tropical Crops, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737

Received:2017-05-03 Online:2017-11-15 Published:2017-11-25
Supported by:
National Natural Science Foundation of China(31570591,31270643)

摘要/Abstract

摘要： R2R3-MYB类转录因子参与包括逆境胁迫反应等多种生物反应。为鉴定橡胶树中MYB转录因子的结构与功能，从橡胶树叶片中克隆HbMYB62全长的cDNA序列。该基因片段大小为1 013 bp，包含945 bp的ORF，编码314个氨基酸残基。其推导的氨基酸序列具有植物HTH_MYB的特异性结构域，并与拟南芥AtMYB62具有高度相似性。qRT-PCR发现HbMYB62主要在橡胶树花中表达，而在树皮、叶和乳胶中表达量较少。其叶片表达量在过氧化氢（H₂O₂）、脱落酸（ABA）、水杨酸（SA）等处理下显著上调。表明HbMYB62与橡胶树的抗逆反应和激素信号传导过程有关，为进一步研究其结构和功能打下基础。

关键词: 巴西橡胶树, HbMYB62, 机械伤害, 激素, 基因表达

Abstract: R2R3-type MYB transcription factor participates in versatile biological process including stress response process. To identify the structure and function of the MYB gene in rubber tree, the full length cDNA of HbMYB62 was cloned in leaves of rubber tree. It was 1 013 bp in length, containing a 945 bp ORF which encodes 314 amino acid residues. The deduced amino acid sequence had the specific domains of plant HTH_MYB DNA binding domain. It showed the highest identity with AtMYB62 in all AtMYB family. This gene is mainly expressed in flower, whilst less expressed in bark, latex and leaf. The gene expression of HbMYB62 were differentially upregulated by H₂O₂, ABA, and SA. The HbMYB62 gene may play an important role in stress resistance and plant hormones signal transduction pathway in rubber tree. This study provides a fundamental knowledge for further study the structure and functions of HbMYB62.

Key words: Hevea brasiliensis, HbMYB62, wounding, hormone, gene expression

中图分类号:

S794.1

陆燕茜, 张冬, 王立丰, 王纪坤. 巴西橡胶树HbMYB62转录因子基因的克隆和表达分析[J]. 植物研究, 2017, 37(6): 953-960.

LU Yan-Xi, ZHANG Dong, WANG Li-Feng, WANG Ji-Kun. Cloning and Expressional Analysis of HbMYB62 under Multi-stimulation in Hevea brasiliensis Muell. Arg.[J]. Bulletin of Botanical Research, 2017, 37(6): 953-960.

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巴西橡胶树HbMYB62转录因子基因的克隆和表达分析

Cloning and Expressional Analysis of HbMYB62 under Multi-stimulation in Hevea brasiliensis Muell. Arg.

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