三色(Bougainvillea peruviana‘Thimma’)在转录水平的甜菜色素和类黄酮积累比较

doi:10.7525/j.issn.1673-5102.2017.02.013

植物研究 ›› 2017, Vol. 37 ›› Issue (2): 249-258.doi: 10.7525/j.issn.1673-5102.2017.02.013

三色(Bougainvillea peruviana‘Thimma’)在转录水平的甜菜色素和类黄酮积累比较

徐夙侠¹, 黄青云¹, 林春松¹, 黄一锦², 胡欧¹

1. 福建省亚热带植物研究所植物生理与生化重点实验室, 厦门 361006;
2. 厦门大学附属医院, 厦门 361003

收稿日期:2016-07-11 出版日期:2017-03-15 发布日期:2017-03-11
作者简介:XU Su-Xia(1973-),female,Ph.D.,Associate Professor,Major in Plant secondary motablisms.
基金资助:
The National Natural Science Foundation of China(Grant No.31071814;31372093);Xiamen Creation Plantform of Science&Technology(3502Z20131004)

Comparison between Betalain and Flavonol Accumulation in Tricolor Bougainvillea peruviana ‘Thimma’ Based on Transcriptome

XU Su-Xia¹, HUANG Qing-Yun¹, LIN Chun-Song¹, HUANG Yi-Jin², HU Ou¹

1. Key Laboratory of Plant Physiology & Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361006;
2. The First Affiliated Hospital of Xiamen University, Xiamen 361003

Received:2016-07-11 Online:2017-03-15 Published:2017-03-11
Supported by:
The National Natural Science Foundation of China(Grant No.31071814;31372093);Xiamen Creation Plantform of Science&Technology(3502Z20131004)

摘要/Abstract

摘要： Bougainvillea peruviana‘Thimma’属于三角梅属，该属植物积累甜菜色素而不是像绝大多数高等植物一样积累花青素。该材料特征同株出现3种颜色：白色、洋红色和白/洋红相间。本研究首次使用3种花色特征的花序（红色Yp、混合色的Ym、白色Yw）作为研究材料进行高通量测序。并通过real-time PCR方法对探测到的花色代谢基因进行验证。共获得平均长度为616 bp的73 325条基因。3种材料的差异显示基因（DEGs）中有327个被注释到甜菜色素合成基因，308个被注释到类黄酮合成基因，466个被注释到花青素合成基因。我们选出8个基因：4个甜菜色素合成基因（PPO，CYP76AD1，cDOPA-5-GT，DODA）和4个花青素合成基因（FLS，DFR，LDOX，3-GT）进行验证。其中，4个甜菜色素合成基因在3种花色材料中的表达较好的正相关于甜菜色素含量。花青素合成途径末端的3个基因（DFR，LDOX，3-GT）在B.peruviana中首次被验证。real-time PCR的验证结果很好的吻合转录组测序的结果。同时，B.peruviana也提供了一个很好的三角梅属植物的生理、生化和分子生物学研究的工具，有效的摒除其他生物学干扰。

关键词: Bougainvillea peruviana, 转录组, Real-Time PCR, 甜菜色素, 类黄酮

Abstract: Bougainvillea peruviana ‘Thimma’, accumulating betalains, not anthocyanins occurring in majority of plants. The cultivar was characterized with either white, magenta, and white-magenta(variegated) bracts in the same branches. We first used magenta(Yp), bicolor(Ym) and white(Yw) open inflorescences for transcriptome analysis by sequencing. Candidate genes involving in pigment metablisms were validated by real-time PCR. We obtained 73,325 genes with average length of 616 bp. Out of DEGs, 327 candidate genes of betalain-biosynthesis, 308 flavonoid-biosynthesis genes and 466 anthocyanin-accumulation genes were detected. Eight candidate pigment-related genes were verified, four were responsible for betalain production(PPO, CYP76AD1, cDOPA-5-GT, DODA), and four responsible for flavonoids production(FLS, DFR, LDOX, 3-GT). Expression strength of four betalain production genes was well consistent with betalain-accumulation in Yp, Ym and Yw. Full later three anthocyanidin-producing genes(DFR, LDOX, 3-GT) were first verified and the expression varied in three samples. Expression of all eight candidate genes was well consistent with RNA-sequencing data. B.peruviana could provide a good tool to study physiological, phytochemical and molecular mechanisms in Bougainvillea, effectively eliminating the interference of some factors.

Key words: Bougainvillea peruviana, transcriptome, real-time PCR, betalain, flavonol

中图分类号:

徐夙侠, 黄青云, 林春松, 黄一锦, 胡欧. 三色(Bougainvillea peruviana‘Thimma’)在转录水平的甜菜色素和类黄酮积累比较[J]. 植物研究, 2017, 37(2): 249-258.

XU Su-Xia, HUANG Qing-Yun, LIN Chun-Song, HUANG Yi-Jin, HU Ou. Comparison between Betalain and Flavonol Accumulation in Tricolor Bougainvillea peruviana ‘Thimma’ Based on Transcriptome[J]. Bulletin of Botanical Research, 2017, 37(2): 249-258.

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三色(Bougainvillea peruviana‘Thimma’)在转录水平的甜菜色素和类黄酮积累比较

Comparison between Betalain and Flavonol Accumulation in Tricolor Bougainvillea peruviana ‘Thimma’ Based on Transcriptome

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