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植物研究 ›› 2015, Vol. 35 ›› Issue (4): 559-563.doi: 10.7525/j.issn.1673-5102.2015.04.014

• 论文 • 上一篇    下一篇

武夷山地伯乐树种群遗传多样性的ISSR分析

林乐静1,2;金楚齐1;郑春豪3;周亚美1;周盈1   

  1. 1.宁波城市职业技术学院,宁波 315100;
    2.宁波市园林植物开发重点实验室,宁波 315502;
    3.浙江省遂昌县林业局,遂昌 323300
  • 出版日期:2015-07-20 发布日期:2016-03-09
  • 基金资助:
     

ISSR Analysis on the Genetic Diversity of Relic Plant Bretschneidera sinensis from Wuyishan Region

LIN Le-Jing1,2;JIN Chu-Qi1;ZHENG Chun-Hao3;ZHOU Ya-Mei1;ZHOU Ying1   

  1. 1.Ningbo City College of Vocational Technology,Ningbo 315100;
    2.Ningbo Key Laboratory of Landscape Plant Development,Ningbo 315502;
    3.Suichang Forestry Bureau fo Zhejiang Province,Suichang 323300
  • Online:2015-07-20 Published:2016-03-09
  • Supported by:
     

摘要: 伯乐树(Bretschneidera sinensis Hemsl.)为我国特有单型科珍稀濒危植物,具重要的科研价值。本研究采用ISSR分子标记对武夷山脉分布的5个伯乐树天然种群和1个移栽种群进行遗传多样性分析。结果表明伯乐树物种水平遗传多样性较高(PPB:75.70%;HES:0.304 5;H:0.450 1),种群水平则较低(PPB:60.13%;HEP:0.238 1;H:0.347 5),MJY种群和BSZ种群分别是所有种群中遗传多样性最高和最低的。5个地理种群间遗传分化程度较高(Gst=0.218 1),原因可能源于伯乐树的繁殖方式及生境片段化,Mantel检验也证实了地理距离与遗传距离具有显著相关性(r=0.626 7,P<0.05)。针对伯乐树种群遗传多样性现状,建议加强现有自然种群的就地保护,促进种群自然更新。

关键词: 伯乐树, ISSR, 遗传多样性, 遗传结构

Abstract: Bretschneidera sinensis Hemsl., with important research value, is an endangered species endemic in East and South Chin. We used inter-simple sequence repeat(ISSR) markers studied the genetic diversity within and among six populations of B.sinensis. The genetic diversity at species level was high(PPB: 75.70%; HES: 0.304 5; H: 0.450 1). However, relatively low genetic diversity existed within populations. Population in Minjiangyuan(MJY) exhibited the greatest level of variability(PPB: 60.13%; HEP: 0.238 1; H: 0.347 5), whereas the population in Baishanzu(BSZ) showed its own variability at the lowest level(PPB: 44.86%; HE: 0.173 6; H: 0.255 6). By the genetic analysis of the ISSRs, we revealed a relatively high level of genetic differentiation among populations by Nei’s gene diversity statistics(21.81%), Shannon’s information measure(22.79%) and analysis of molecular variance(AMOVA)(18.62%). The main factors responsible for the relatively high level of differentiation among populations were probably related to the predominant outcrossing reproductive system and recent habitat fragmentation, and a significant correlation was between genetic distance and geographic distance(r=0.626 7, P<0.05). The high diversity would hold promise for conservation and restoration efforts to save the endangered species from extinction.

Key words: Bretschneidera sinensis, inter-simple sequence repeats(ISSR), genetic diversity, genetic structure

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