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    15 June 2003, Volume 23 Issue 2
    A NEW SPECIES OF MANGLIETIA (MAGNOLIACEAE) FROM SE YUNNAN IN CHINA
    SHUI Yu-Min, CHEN Wen-Hong
    2003, 23(2):  129-130. 
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    A new species of the genus Manglietia Blume (Magnoliaceae), M. hongheensis Y. M. Shui et W. H. Chen, from SE Yunnan in China is described.
    A NEW SPECIES OF PODOCARPIUM FROM YUNNAN PROVINCE
    QIAN Yi-Yong
    2003, 23(2):  131-132. 
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    Podocarpium lancangense Y. Y. Qian, sp. nov. is reported.
    NEW TAXA OF MAZUS AND ROSA FROM YUNNAN PROVINCE
    DONG Xiao-Dong, LI Ji-Hong
    2003, 23(2):  133-133. 
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    A new specie of Erigeron breviscapus and a new forma of Roscoea tibetica is repoted.
    TWO SPECIES OF GENUS SCAPANIA (HEPATICAE;SCAPANIACEAE) NEW TO CHINA
    CAO Tong, YU Jing, SONG Guo-Yuan
    2003, 23(2):  134-136. 
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    Two species of genus Scapania (Hepaticae, Scapaniaceae) new to China found from Si-chuan Province are reported. They are Scapania cuspiduligera(Nees) K. Muell. And S. glaucocephala(Tayl.) Aust.
    THREE SPECIES OF MYRIACTULA (LEATHESIACEAE, PHAEOPHYTA)NEW TO CHINA
    LUAN Ri-Xiao
    2003, 23(2):  137-140. 
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    Three species of Myriactula new to Chian, M. arabica (Kuetzing) Feldmann, M. clavata (Takamatsu) Feldmann and M. sargassi (Yendo) Feldmann were reported.
    A NEW VARIETY OF IMPATIENS LINN. FROM ZHEJIANG PROVINCE
    JIN Xiao-Feng, ZHANG Fang-Gang
    2003, 23(2):  141-142. 
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    A new variety of Impatiens was described.
    A NEW RECORD OF TRITOMARIA(HEPATICAE)FROM CHINA
    SUN Jun, GAO Qian, CAO Tong
    2003, 23(2):  143-145. 
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    A new record form Tritomaria quinquuedentata f. gracilis (Jens.) Schust. from China is reported, and the taxonomic situation of the genus Tritomaria is also discussed.
    NOTES ON LEGUMINOSAE OF FLORA OF CHINA IN CHINESE EDITION
    ZHU Xiang-Yun, Hiroyoshi OHASHI
    2003, 23(2):  146-146. 
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    Two new synonyms, Chesniella mongolica (Maxim.) P. C. Li (1993) and Tadehagi pseud-otriguetrum (DC.) Yang & Huang (May, 1995), are proposed in this paper, which should be rejected and replaced by Chesniella mongolica (Maxim.) Boriss (1964) and Tadehagi pseudotriguetrum (DG.) H. Ohashi (April, 1995) respectively.
    A NEW SPECBES OF ELACHANTHEMUM AND DISCUSSION ON THE CLASSIFICATION AND EVOLUTION OF THE GENUS
    ZHU Zong-Yuan, LIANG Cun-Zhu, WANG Wei, LIU Zhong-Ling
    2003, 23(2):  147-153. 
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    A new species-Elachanthemum polycephalum Z. Y. Zhu et C. Z. Liang, -is de-scribed in detail and the problem of the classification position of Elachanthemum is discussed by mor-phological characteristics, ecological properties, geographical distribution, and flora origin. Morpho-logically, although Elachanthemum is similar to Stilpnolepis, there are obvious differences on the mor-ph and structure of flowers, achenes and pollen grains between two genera. Therefore, the Elachanthemum is an independent genus and can not be merged into Stilpnolepis. The genus is a primitive genus in the category of Artemisia, and it, perhaps, come from the Pro-Artemisia that distributed in the northem part of Asia in tertiary. It is a relict xerophytic element in the eastern part of Tethys, and is an endemic genus in the desert of Asia Media (Gobi desert). The discovery of the new species in Elachanthemum manifest that the species differentiation are stili progressing in desert flora, and make the Elachanthemum from a monospecific genus to olig-specific one (2 species).
    STUDEES ON THE GAMETOPHYTES DEVELOPMENT IN FERNS
    ZENG Han-Yuan, DING Bing-Yang
    2003, 23(2):  154-158. 
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    The gametophytes developments of 21 fems are researched. They are Angiopteris fokiensis, Osmunda vachellii, Osmunda banksiifolia, Alsophila denticulate, Pteris excelsa, Pteris vittata, Asplenium wrightii, Blechnum orientale, etc. It suggests these:(1) The cluster analysis with UPGMA on the gametophytes characters of 21 fems indicates that the ferns which have close genetic relationship in QIN Ren-Chang classify system(1978) are resemble in gametophytes characters, But there are some exception cases. (2)The gametophytes development of different species has many similar features, but there are many different features, too. (3)Some characters of gametophytes are stable, which can be used as classify evidence.
    STUDIES ON SPORES CULTURE AND DEVELOPMENTAL BIOLOGY OF ATRICHUM UNDULATUM
    LIU Bao-Dong, CONG Ying-Zhi
    2003, 23(2):  159-163. 
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    Artificial culture of unicell spore of Atrichum undulatum (Hedw.) P. Beauv is performed successfully in this paper, and the authors research the whole process of that and take the photoes. through the research, we find that its spores have no post-maturation or domant period. They can germinate after 3-4 days and the rate of germination is over 95%. About 10 days later, its chlorone-mata, caulonemata and rhizoides begin to differentiate, almost on the 40th day bud primordium ap-pears, and then, around the 50th day, bud forms gradually. At last, nearly the 80th day, the whole plant finishes its vegetive growth and starts development of sexual organs. Its primary rhizoides devel-op poorly, and basai celis have no division, the way of chloronema's generating caulonema is complex and caulonema cell has three kinds of diastemas; spherical, declinate and transuerse, at the beginning of the differentiation of bud primordium, caulonema generates cell population that have high photosyn-thetic capacity, and bud primordium on its base. The rate of successful differentiation of the bud primordium is about only 50%, once bud forms, protonemata always perishes and specializes in rhizoides. The research discussed the morpha development, phyoiological ecology, generation, evolution and so on.
    ANATOMICAL STUDIES ON OROSTACHYS FIMBRIATUS FROM CRASSULACEAE
    ZHENG Yan, GONG Jie, CHEN Shi-Chao, LI Ling
    2003, 23(2):  164-168. 
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    The stem structure, leaf epidermis structure and the pollen morphology of a traditional orien-tal medical herb Orostachys fimbriatus (Crassulaceae) were examined in detail under light microscope and scanning electron microscope and compared with those of Sedum of the same family. According to our anatomical studies, the stems of Orostachys fimbriatus and Sedum spp. consisted of epidermis, cortex and primary vascular tissue. They differed mainly in the size of parenchyma cells, the amount of idio-blasts and the type of vascular bundles. On leaf epidermis, the two distinguished in the shape of surface celis, anticlinal wall and the ornamentation of cuticle while they shared the same type of stomata, aniso-cytic type. As far as pollen morphology was concerned, the two had much the same characteristics such as the pollen shape, angulaperturate, 3-colporate and ornamentation type on the exine surface. But the size and the thickness of exine wall differed distinctly. Some marked differences are taxonomically useful for the identification of genera and division of family. At the end of the article, the author concludes that Orostachys is superior to Sedum spp..
    HISTOLOGICAL STUDY ON ADVENTITIOUS BUD FORMATION AND SOMATIC EMBRYOGENESIS OF PEANUT (ARACHIS HYPOGAEA L.) LEAFLETS
    LIN Rong-Shuang, WANG Qing-Hua, LIANG Li-Kun, XIAO Xian-Hua
    2003, 23(2):  169-171. 
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    This paper deals with a histological observation of adventitious bud formation and somatic embryogenesis of peanut (Arachis hypogaea L.) leaflets. Adventitious buds and embryoids developed from the leaflets of 4-day-old seedlings on MS basai medium supplemented with TDZ 0. 2 mg/L and NAA 0.4 mg/L, and developed into plantlets after they were transferred onto MS medium without any phytohormone. Histological observations showed that both adventitious buds and somatic embryos developed from the surface of the callus. However, adventitious buds originated from cell mass while somatic embryos originated from one single embryogenic cell. The somatic embryos developed directly into plantlets with roots and buds through multicellular proembryo, globular, heart-shaped, torpedo-shaped and cotyledon stages.
    EFFECT OF STEM EXTRACTS OF EUCALYPTUS LH22 AND U6 CLONES ON THE ROOTING OF MUNG BEAN CUTTINGS AND ON GERMINATION OF SEEDS
    ZHAN Fu-Jian, WU Guang-Hong, HUANG Zhuo-Lie, TAN Shao-Man, OU Jiang-Yi, ZHOU Guang-Hui
    2003, 23(2):  172-177. 
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    The results of this investigation indicated that the crude stem extract of Eucalyptus LH22 clone (Eucalyptus urophylla S. T. Blade Eucalyptus camaldulensis Dehnhardt), which was difficulty-to-root, inhibited evidendy the rooting rate and the length of roots of mung bean cuttings. Other-wise, there was no case in the U6 clone (Eucalyptus urophylla S. T. Blade U6 clone), which was easy-to-root. The crude extractions were purified by organic solvent extraction methods, and then all kinds of extractions were tested by seed germination. The experiments revealed that there were stron-ger inhibitory ingredients existed in water extract and ether extract by analysis of Duncan's SSR. But there was no inhibiting effect in the extract of U6 clone. The inhibiting fractions were purified further by silica gel column chromatography and the result showed that different inhibitory activities were exis-ted among the several purified fractions. So, It's concluded that there were several different ingredi-ents of inhibitors in the stem of LH22 clone and less inhibitors in the steni of U6 clone.
    STUDY ON OPTIMIZATION OF THE CULTURE MEDIUM FOR ORIENTAL HYBRIDS POLLEN GERMINATION
    CHE Dai-Di, FAN Jin-Ping, WANG Jin-Gang
    2003, 23(2):  178-181. 
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    Having adopted the six species pollen of oriental hybrids and twenty-two formulation of culture medium. We studied the effect of sucrose and boric acid concentration on the pollen germination and pollen tube elongation, and further optimized the isolated culture medium for oriental hybrids pollen germination. The result indicated that the optimum composition of isolated culture medium for oriental hybrids pollen germination was 13% sucrose+143 mg·kg-1 boric acid +1.0% agar.
    STUDY ON EXTRACTION OF SYRINGIN BY ULTRASONIC FROM ACANTHOPANAX SENTICOSUS
    LI Qing-Yong, FU Yu-Jie, LÜ Xin, ZHANG Ying, ZU Yuan-Gang
    2003, 23(2):  182-184. 
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    The optimal conditions of extraction of syringin by ultra sonic wave from Acanthopanax senticosus using experimental design were studied. The optimal conditions are as follows:the solvent is water, the sonic extraction time is 200 min, the ratio of extracted solvent is 8 ml/g raw material time, and the extraction temperature is room temperature.
    STUDY ON THE CONTENT OF CHLOROGENIC ACID FROM AMBROSIA ARTEMISIIFOLIA L. BY HPLC
    ZHAO Xin, YUAN Xiao-Ying, FU Yu-Jie, ZHANG Ying
    2003, 23(2):  185-188. 
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    Chologenic acid content in Ambrosia artemisiifolia L. stem was studied by the method of high performance liquid chroma graphy. Analysized sample made from Ambrosia artemisiifolia L. stem was through ultrasonic extraction in distilled water. The result showed that we got a good extrac-tion yield of chlorogenic acid under the optimal condition of 60℃ temperature and 70 minutes.
    EFFECT OF DROUGHT STRESS ON PROLIN ACCUMULATION OF ALFALFA
    LI Bo, JIA Xiu-Feng, BAI Qing-Wu, TANG Yu-Hong
    2003, 23(2):  189-191. 
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    This experiment studies the influence of drought stress on Alfalfa proline accumulation in the imitating drought condition of PEG-6000 solution. It show the highest free proline accumlation appears in 20%PEG solution and after 48 hours; the Alfalfa(No. 1 zhongmu) has the highest accumlation. The experiment shows that proline accumulation appears in drought condition.
    DETERMINATION OF FLAVONOIDS IN GLYCYRRHIZA URALENSIS FISCH WITH ULTRAVOBLET SPECTROPHOTOMETRY
    LU Xin, FU Yu-Jie, WANG Wei, ZU Yuan-Gang
    2003, 23(2):  192-194. 
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    The concentration of flavonoids in Glycyrrhiza uralensis fisch was determined with ultravio-let spectrophtometry, the detective wavelength was 500 nm, the R2 of Standard curve was 0.9994 and the relative Standard deviation was 0.20% with the average recovery of 100.20%. The result showed that the rate of veracity of this method was high and this provided the determination of Glycyrrhiza flavonoids of pharmacognostic and finished products a feasible method.
    THE SOLUBLE PROTEIN ANALYSIS OF HYBRID LARCH DURING CUTTING ROOTINGS
    LIU Gui-Feng, ZHUANG Zhen-Dong, YOU Xiang-Ling, JIANG Jing
    2003, 23(2):  195-197. 
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    The soluble proteins of hybrid larch (Larix olyensis× Larix kaempferi) during cutting ro-otings were analysed by SDS polyacrylamide electrophoresis (SDS-PAGE). From the SDS-PAGE result, we can infer that three kind of proteins (24 KD, 26 KD, 39 KD) are connected with generation and differentiation of root primordium; The protein with 28 KD molecular weight has an ability to promote the root primordium growing and generating adventitious roots; The protein with 47 KD molecular weight inhibits the generation of root primordium or the growth of adventitious roots.
    THE STUDY ON AGROBACTERIUM RHIZOGENES MEDIATED CHITINASE AND β-1, 3-GLUCANASE TRANSFORMATION FOR TOBACCO
    ZHANG Yan-Ni, QU Min, XU Xiang-Lang, LI Xin-Ling
    2003, 23(2):  198-203. 
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    The plasmid pBLGC which constained chitinase gene and β-1, 3-glucanase gene and NTP-II gene (resistansing kanamycin) was transformed into Agrobacterium rhizogenes with root-in-ducing plasmid by using freeze-thaw method and triparental crossing method. Chitinase gene and β-1, 3-glucanase gene were placed under the control of the Cauliflower MosaicVirus 35S promoter and introduced into three varieties of tobacco by Agrobacterium rhizogenes mediated leaf-plated trans-formation method. 126 regenerated plants were selected by Kanamycin. Among them, 119 plants have been tested by 35S, chitinase gene and β-1, 3-glucanase gene primer separatly through PCR elec-trophoresis assay, of which 72 plants showed chitinase gene positive, 47β-1, 3-glucanase gene postive and 36 postive plants containing both chitinase gene and β-1, 3-glucanase gene. The trans-ferring frequency of foreign gene has relation with plant variety and the length of foreign gene. Chitinase gene seemed to inherit easier than β-1, 3-glucanase gene. 24 plants among PCR positive plants have been carried out by PCR-Southern. The result was that they showed positive. The study indicated chitinase gene and β-1, 3-glucanase gene respectively or together introducing and cointe-grating into plant genenome.
    RELATIONSHBP BETWEEN DESICCATIONTOLERANCE AND SUGARS IN SEEDS
    YANG Qi-He, SONG Song-Quan, YE Wan-Hui, YIN Shou-Hua
    2003, 23(2):  204-210. 
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    Accumulation of sugars in plant seeds varies with developmental stages and seed categories and relates to seed desiccation-tolerance. Some certain sugars accumulate following with deveiop-ment of many plant seeds, which is considered to play an import role in acquisition of desiccation-tolerance. But effects of sugars on seed desiccation-tolerance are not independent but interact-with those of ABA, proteins et al. The desiccation-tolerance is correlated to not only categories and content of sugars, but also to physiological status and developmental courses. The research advances on relationship between seed desiccation-tolerance and sugar is reviewed in this paper.
    THE EFFECT ON HUMUS AND SOME ENZYMES' ACTIVITY IN ALKALI SOIL BY DIFFERENT AMELIORATIVE WAYS
    YUE Zhong-Hui, SUN Guo-Rong, YAN Xiu-Feng
    2003, 23(2):  211-214. 
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    Based on the study of humus and some enzymes' activity in alkali soil treated by different ameliorative ways such as shallow ploughing, manuring phosphogypsum, furfural residue, organic fer-tilizer, constructing manual meadow of Puccinellia tenuiflora or P. tenuiflora-Leymus chinensis and so on, the result show that the content of humus, polyphenol oxidase, cellulase, hydrogen peroxidase and peroxidase activity increase with different degree treated by different ameliorative ways. Further-more, there are well correlations between the content of humus and cellulase activity, between cellulase and hydrogen peroxidase activity.
    THE STUDIES ON ALLOZYME OF SEVEN SPECIES IN TYPHONIUM SCHOTT AND TWO CLOSE GENERA
    BIAN Fu-Hua, WANG Zhong-Lang, GUAN Kai-Yun
    2003, 23(2):  215-219. 
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    Six enzyme systems of seven species in Typhonium and three in two close genera were ana-lyzed by means of electrophoresis. Seven allozyme sites were checked. Tree diagram was drawn using BIOSYS-2 for cluster analysis of genetic similarity. Seven species of Typhonium were divided into three groups and the relationship between Typhonium, Sauromatum Schott and Pinellia Tenore was clarified. Namely Typhonium is closer from Sauromatum than Pinellia.
    CLUSTER ANALYSIS FOR KARYOTYPE OF ASTRAGALUS PENDULIFLORUS LAM. COMPLEX(LEGUMINOSAE)
    SHI Gang-Rong
    2003, 23(2):  220-223. 
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    On the basis of average Euclidean distance coefficient, using UPGMA, Q cluster analysis for karyotype of 11 populations in 5 taxa of Astragalus penduliflorus Lam. Complex have been conduc-ted. The results show that the karyotype of the eight populations of A. penduliflorus Lam, A. membranaceus var. membranaceus, A. minhensis and A. membranaceus var. purpurinus are vary in contin-uous pattern, while they are distinguished to the three population of A. membranaceus var. monghulicus in a interval pattern. As a result, we proposed that A. pendulifloms Lam should be treated as the synonym of A. membranaceus, and the complex might include two species(A. membranaceus(Fisch) Bunge and A. monghulicus Bunge), two subspecies (A. membranaceus spp. Membranaceus and A. membranaceus spp. Minhensis), and one variety (A. membranaceus var. purpurinus). The evolution of the karyotype of Astragalus pendulifloms Lam. Complex is consistent to Stebbins who regarded the evolution of the Karyotype in plants is from symmetry to dissymmetry.
    STUDY ON THE GENETIC DIVERSITY OF PICEA KORAIENSIS
    ZHANG Han-Guo, SUN Li-Fu, HAN Ji-Feng, FENG Bao-Lin
    2003, 23(2):  224-229. 
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    Twelve populations of Picea koraiensis are collected typically within its distribution from Changbai Mountains, its branches Zhangguanchai Mount, Laoyie Mount and Wanda Mount, to Xiaox-ing'an Mountains and Dasing'an Mountains, with interspersed areas of internai Mogul grassland and Sikshote Mountains. The theories and methods of genetics are applied which include Population Genetics, Ecological Genetics, Physiological Genetics, Molecular Genetics, Forest Genetics and Forest-ry Breeding as well as Statistics such as PCA and Cluster. The variations within population and among populations are systematically studied from phenotype to molecular Ievel and the relationship among the characters of various parents and their progenies, feature in allozyme and their relative ecological environment factors are analyzed. The results indicate as follows:it is first in parent bio-system characters, the morphology, growth, bio-mass and mineral elements of progeny seedlings that the genetic variation of Picea koraiensis is conducted. There are abundant variations in it and its genetic variation within a population is larger than among populations. Among 21 gene loci in 11 enzymes testing, poly-morphic loci makes up about 27.2 percent of total. The variation within population is 15.2 percent and among populations is 84.2 percent. In contrast with other species in Picea spp., the ratio of major allozyme loci within population of Picea koraiensis belongs to lower level, while that among populations reaches to higher Ievel. The genetic variations in allozyme, comparing with morphology, show a similar tendency. According to the characters of parent and progeny of Picea koraiensis and their relative climatic factors, the area of seed allocation are divided into six sections; Ⅰ Changbai Mountains area, Ⅱ Laoyie Mount-Wanda Mount area, Ⅲ Xiaoxing'an Mountains area, Ⅳ North part of Daxing' an Mountains, V Southwest part of Daxing' an Mountains, Ⅵ Sikshote area.
    A STUDY ON THE SCHIMA SIPERBA COMMUNITY IN GUTIAN MOUNTAIN NATURE RESERVE IN ZHEJIANG PROVINCE
    HU Zheng-Hua, YU Ming-Jian, DING Bing-Yang, FANG Teng, CHEN Qi-Chang
    2003, 23(2):  230-236. 
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    The study on the features showed:There are various plants in the community of Schima superba, and its family and genus are of scattered composition. Their florai composition is complicat-ed. In terms of genus geographical elements, many of them are distributed in tropical zones. Accord-ing to Raunkiaer's statistics of life-form, the life-form phaenerophyte of this community numbers first, and the geophyta second. The features of the community leaf is mainly microphyll, simple, her-baceous and unentire. The vertical structure of community is relatively complicated which can be di-vided into tree layer, shrub layer and herb layer as well as a definite number of interstratum plants. The species diversity index of different layers shows:shrub layer> tree layer> herb layer.
    THE STUDY OF INDIVIDUAL TREE MODEL ON LARIX OLGENSIS PLANTATION
    LIU Zhao-Gang, LI Feng-Ri, YU Jing-Cheng
    2003, 23(2):  237-244. 
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    Using 66 temporary plots, 18 permanent plots, and 8 branch analysis plots of Larix olgensis plantations, growth of dominant trees and the crown structure and dynamics of trees were analyzed. The Korf equation, which is suitable for tree growth, was used to develop potential growth function of trees. Stand density index (SDI) was applied as a quantitative index to measure mean crowd degree between trees. On selecting individual tree competition indices, tree crown variables were introduced and it was compared with conventional competition indices in order to downplay distance factor. Using relative crown surface area as the distance-independent competition indices, individual tree growth modei of Larix olgensis was developed.
    THE ESTIMATING OF THE FRUIT RESERVES OF SCHISANDRA CHINENSIS IN THE NORTH -EAST REGION BY THE METHOD OF ARTIFICIAL NEURAL NETWORK
    NIE Jiang-Li, CONG Pei-Tong, ZU Yuan-Gang, CAO Jian-Guo
    2003, 23(2):  245-251. 
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    Schisandra chinensis (Magnoliaceae) is valuable medical materials. Because the relation between fruits set of S. chinensis and component of validity and geographic environment is very close, we selected three representative regions in the Northeast of China which grows 5. chinensis:Chang Bai Shan region (Liao Ning province Xin Bin country), Zhang Guang Chai Ling region (Wei He for-estry bureau), Xiao Xing An Ling region (Yi Chun Tao Shan forestry bureau) for studying. In present paper the neural network teehnology was adopted. The artificial modei of the relation betvveen fruits set and geographic environment were built. Basing on this modei we researched relation betvveen mac-roscopic fruits set of S. chinensis and geographic environment. The results indicated that the relation between temperature and fruits set is most closed. The fruits set of 5. chinensis that distributes in regions and reserves of resources were provided and new method that calculates reserves of resources was provided. Through calculating and analyzing we conclude that Xiao Xing An Ling region with higher fruits set is more feasible area that S. chinensis grows.
    GIS APPLYING IN COUPLE MAP OF CITY WATER AND ECOLOGICAL ENVIRONMENT
    ZHU Xue-Qin, CONG Pei-Tong, WEN Li-Jie
    2003, 23(2):  252-256. 
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    Geographic Information System, GIS-Mapinfo, applying in city ecology. Compiling cou-ple map of city water and ecological environment can be provided for city water resources develop-ment, territory plan, environment protection and city plan.