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Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (4): 428-433.doi: 10.7525/j.issn.1673-5102.2010.04.009

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Effect of Various Hormones with Different Concentration Ratios on the Regeneration Plant from Tender Petioles of Rosa marretii Levl.

GU Di-Zhou;GAO Han-Dong*;WANG Yu-Fang;FENG Ying   

  1. 1.Department of Biology,Tonghua Normal University,Tonghua　134002；2.College of Forest Resources and Environment Nanjing Forestry University,Nanjing　210037；3.Supervision and Testing Center for Special Economic Animal and Plant Product Quality Ministry of Agriculture P.R.China,Jilin　132109
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20
  • Contact: GAO Han-Dong
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Abstract: The tender petioles of Rosa marretii Levl. was used as explant. Uniform design was used for selecting the most suitable media for callus induction from tender petioles, callus redifferentiation and regeneration shoots rooting. The results showed that LS+6-BA 1.80 mg·L-1+NAA 0.10 mg·L-1+2,4-D 0.08 mg·L-1 was suitable for callus induction with the induction rate of callus being 99.0%; LS+6-BA 3.45 mg·L-1+NAA 0.05 mg·L-1 was suitable for callus redifferentiation with the differentiation rate of callus being 99.5%; 1/2LS(macro-elemenyts)+IAA 0.05 mg·L-1 was suitable for rooting with the rate of rooting being more than 98.0%.Transplant success ratio was more than 96.0%. The experiment results proved that plant regeneration system has been successfully established from the tender petioles of R.marretii Levl..

Key words: Rosa marretii Levl., petiole, callus, regeneration plant, uniform design

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