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Bulletin of Botanical Research ›› 2007, Vol. 27 ›› Issue (2): 212-217.doi: 10.7525/j.issn.1673-5102.2007.02.019

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Cloning and Sequence Analysis of rd22 Gene from Polygonum sibricum

ZHENG Lei;LIU Guan-Jun;YANG Chuan-Ping*   

  1. Key Laboratory of Forest Genetics and Tree Breeding of Heilongjiang Province,Northeast Forestry University, Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-03-20 Published:2007-03-20
  • Contact: YANG Chuan-Ping
  • Supported by:
     

Abstract: Using RACE (rapid amplification of cDNA ends) method, the full-length cDNA of dehydration-responsive protein RD22 was cloned from Polygonum sibricum treated with 3%NaHCO3 solution for 48. The sequence analysis shows that the rd22 gene is 1 302 bp in length, including 59 bp of 5′untranslated region, 25 bp of 3′untranslated region and 1 218 bp of open reading frame (ORF).The rd22 gene encodes a protein of 405 amino acids. The C-terminal of the RD22 protein contains a conserved BURP domain, and the N-terminal contains 5 copies of a repetitive sequence of THV-VGKGGV-V. Signal peptide prediction shows the RD22 protein is a secretory protein, and has a signal peptide structure at the first 21 amino acids region. The amino acid sequence of RD22 prutein from Polygonum sibricum has a high homology with grape of 60%. This rd22 gene has been accepted by GenBank, and the accession number of gene sequence is DQ836050.

Key words: Polygonum sibricum, rd22 gene, cloning, sequence analysis

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