Construction of cDNA Library in Florescence and Screening Specifically Expressed Genes in Sugar Beet M14

doi:10.7525/j.issn.1673-5102.2008.04.006

Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (4): 408-411.doi: 10.7525/j.issn.1673-5102.2008.04.006

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Construction of cDNA Library in Florescence and Screening Specifically Expressed Genes in Sugar Beet M14

MA Chun-Quan;ZHANG Ying;CUI Ying;WANG Bing;WANG Yu-Ting;LIU Jin-Ling;WANG Hong-Jian;LI Hai-Ying*

(Bio-Molecular Lab of College of Life Sciences，Heilongjiang University，Harbin　150080)

Received:1900-01-01 Revised:1900-01-01 Online:2008-07-20 Published:2008-07-20
Contact: LI Hai-Ying
Supported by:

Abstract

Abstract: The cDNA library of sugar beet M14 in florescence was constructed using ZAP expression vector. Two ESTs, which were specifically expressed in M14 and obtained by suppression subtractive hybridization, were used as probes to screen the cDNA library. Then, two cDNA fragments Me-84 and Me-86 specifically expressed in M14 were obtained. Full length cDNA sequence of gene M14-86 was later obtained using the method of RACE. Bioinformatic analysis of M14-86 and Me-84 cDNA fragments showed high homology with the 26S ribosomal RNA gene in Sarracenia purpurea and Portulaca grandiflora, respectively.

Key words: cDNA library, EST, expression vector, plaques in situ hybridization, method of RACE

CLC Number:

Q785

MA Chun-Quan;ZHANG Ying;CUI Ying;WANG Bing;WANG Yu-Ting;LIU Jin-Ling;WANG Hong-Jian;LI Hai-Ying*. Construction of cDNA Library in Florescence and Screening Specifically Expressed Genes in Sugar Beet M14[J]. Bulletin of Botanical Research, 2008, 28(4): 408-411.

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Construction of cDNA Library in Florescence and Screening Specifically Expressed Genes in Sugar Beet M14

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