Abstract: To illustrate the effects of several factors on the highfrequency regeneration plant system of Pueraria lobata leaves and stems. Plant tissue culture, orthogonal design and single factor treatment were applied. The best disinfection way of P. lobata leaves and stems was firstly using 70% ethyl alcohol to process 30 s, then using 0.1%HgCl₂ to process 15 min again; The best culture medium of P. lobata leaf callus induction was MS+NAA 1.0 mg·L^-1+2,4-D 2 mg·L^-1; The best culture medium of P. lobata stem callus induction was MS+NAA 1.0 mg·L^-1+6-BA 1.0 mg·L^-1+2,4-D 2 mg·L^-1; The dark culture was more advantageous for P. lobata callus induction; The best sucrose concentration of P. lobata leaf and stem callus induction was 30 g·L^-1 sucrose. The best culture medium of P. lobata leaf callus redifferentiation was MS+NAA 1.0 mg·L^-1+6-BA 3.0 mg·L^-1. The best culture medium of P. lobata stem callus redifferentiation was MS+ NAA 0.5 mg·L^-1+KT 2 mg·L^-1; The light culture was more advantageous for P. lobata leaf and stem callus redifferentiation; The best culture medium of P. lobata leaf callus regenerated buds rooting was MS+NAA 0.5 mg·L^-1+PP₃₃₃ 0.5 mg·L^-1+30 the g·L^-1 sucrose. The best culture medium of P. lobata stem callus regenerated buds rooting was MS+NAA 0.5 mg·L^-1+PP₃₃₃ 3.0 mg·L^-1+30 the g·L^-1 sucrose; 1.0 mg·L^-1 PP₃₃₃ and transplant matrix of vermiculite: perlite (2:1) could remarkably improve the transplant survival rate of P. lobata leaf regeneration seedling, 3.0 mg·L^-1 PP₃₃₃ and transplant matrix of vermiculite: perlite (2:1) could remarkably improve the transplant survival rate of P. lobata stem regeneration seedling.

Key words: Pueraria lobata, leaf, stem, callus, high-frequency regeneration plant