Effects of Different Growth Retardant Treatments on Rooting of Prunus humilis Rhizome Cuttings

doi:10.7525/j.issn.1673-5102.2025.01.010

Abstract

Abstract:

To explore the effect of growth retardants on the rooting of Prunus humili rhizomes cuttings， ‘Jinou 1’ rhizome cuttings were used as materials. Optimal concentrations of growth retardants S₃₃₀₇ （uniconazole）， PP₃₃₃ （paclobutrazol）， and DPC （dikegulac） were screened by measuring rooting rate， germination rate， morphological indices， nutrients， oxidative enzyme activities， endogenous hormones during the rooting process of rhizome cutting. The results showed that the retardants S₃₃₀₇（50 mg?L^-1）， PP₃₃₃（100 mg?L^-1）， and DPC（100 mg?L^-1） significantly increased the rooting rate of P. humilis rhizome from the control of 22.16% to 78.02%， 57.10%， and 56.70%， respectively. During the rooting process of rhizome cuttings， growth retardant treatments also increased the contents of soluble sugars， starch， soluble proteins， and the activity of POD， SOD and CAT. In addition， the retardant treatment promoted the content of IAA and expression levels of IAA synthesis genes ChTAA1， ChTAA2 and ChYUC1 in rhizome cuttings and inhibited the synthesis of GA₃， ABA， and ZT， which was favorable for the rooting of P. humilis rhizome cuttings. This experiment provided a theoretical basis for the propagation of P. humilis rhizome cuttings.

Key words: Prunus humilis, rhizome cutting, growth retardants, nutrients, oxidative enzyme activity, endogenous hormones

CLC Number:

S662.5

Xiaopeng MU, Xiaoyan MOU, Xinliang QI, Jing WANG, Yu YANG, Jiancheng ZHANG, Pengfei WANG. Effects of Different Growth Retardant Treatments on Rooting of Prunus humilis Rhizome Cuttings[J]. Bulletin of Botanical Research, 2025, 45(1): 88-97.

Figures/Tables 10

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基因名称 Gene name	上游引物（5′→3′） Forward primer sequence（5′→3′）	下游引物（5′→3′） Reverse primer sequence（5′→3′）
ChActin	GCAGCGACTGAAGACATACAAG	GGTGGCATTAGCAAGTTCCTC
ChTAA1	TTGTAGTAGCAGGCTGGCAT	TCTTCCTTGAGTCACCGCAT
ChTAA2	ATGCGGTGACTCAAGGAAGA	TGGAAGAGAGTGCGTGAAGT
ChYUC1	CCGAAATGGGAGTGTTGTCC	CACGGTTGTCATCCACAGTC

处理 Treatment	发芽率 Bud germination rate/%	生根率 Rooting rate/%	总根长 Total root length/cm	平均根直径 Average root diameter/mm	根表面积 Root surface area/cm²	根系体积 Root volume /cm³	平均根数 Mean root number	平均根长 Average root length/cm	最大根长 The longest root length/cm	根系效果指数 Root index
CK	44.12±0.05^e	22.16±0.03^e	34.18±1.40^h	1.55±0.01^b	16.85±1.16^h	0.73±0.06^cd	8.14±0.91^d	3.46±0.32^bc	6.87±0.35^bc	0.70±0.06^e
S1	58.33±0.06^bc	43.33±0.04^c	36.60±1.02^h	1.21±0.02^d	8.88±0.66ⁱ	0.30±0.12^e	15.00±1.17^ab	3.97±0.33^b	15.07±1.12^a	1.49±0.23^cd
S2	80.02±0.02^a	78.02±0.02^a	129.11±1.49^c	1.17±0.02^d	47.93±2.00^b	1.51±0.08^a	16.26±0.34^ab	5.33±0.70^a	4.78±0.17^d	2.17±0.33^ab
S3	79.17±0.01^a	63.33±0.01^b	38.66±1.26^h	1.74±0.06^a	22.74±2.82^f	1.04±0.07^bc	14.55±2.01^b	2.83±0.38^c	6.75±0.31^bc	1.04±0.26^de
P1	67.00±0.03^b	57.10±0.01^b	158.23±1.84^b	0.79±0.02^f	38.65±2.53^cd	1.03±0.07^bc	16.22±0.86^ab	5.71±0.54^a	5.94±0.17^c	2.23±0.53^ab
P2	58.33±0.03^bc	35.83±0.03^d	87.34±1.81^d	1.06±0.05^e	29.73±1.19^e	0.61±0.45^d	11.37±3.44^c	3.94±0.22^b	6.32±0.51^bc	1.11±0.27^de
P3	55.12±0.06^cd	22.50±0.02^e	76.45±1.82^e	1.37±0.05^c	35.08±4.69^d	0.80±0.16^bcd	15.47±2.36^ab	4.28±0.42^b	6.94±0.22^b	1.73±0.16^bc
D1	64.00±0.03^bc	56.70±0.03^b	169.62±3.37^a	1.07±0.04^e	59.63±3.48^a	1.51±0.23^a	18.10±0.95^a	5.66±0.51^a	6.97±0.16^b	2.43±0.22^a
D2	56.67±0.03^cd	28.33±0.02^e	80.13±1.64^e	1.42±0.04^c	40.29±4.18^c	1.07±0.04^b	17.22±1.42^ab	2.80±0.36^c	4.26±0.54^d	1.26±0.13^cd
D3	48.33±0.03^de	22.50±0.02^e	57.13±2.19^f	0.6±0.08^h	11.83±1.02ⁱ	0.18±0.02^f	10.36±1.06^cd	2.73±0.48^c	3.83±0.21^d	0.71±0.13^e

处理 Treatment	株高 Plant height/cm	新生枝茎粗 New branch stem diameter/mm	叶形指数 Leaf shape index	叶面积 Leaf area/cm²
CK	20.45±0.65^a	1.75±0.09^b	2.82±0.14^a	4.37±0.15^a
S1	16.47±0.63^bc	1.53±0.21^d	2.46±0.11^bcd	4.20±0.06^ab
S2	16.83±0.55^b	1.52±0.05^d	2.26±0.16^bcd	4.12±0.61^ab
S3	11.76±0.41^f	1.38±0.06^e	2.23±0.05^cd	3.34±0.27^cd
P1	12.15±0.37^e	1.71±0.10^c	2.55±0.21^ab	3.79±0.35^bc
P2	12.05±0.52^e	1.38±0.02^e	2.41±0.22^bcd	3.23±0.39^d
P3	14.66±0.51^d	1.34±0.05^f	2.30±0.07^bcd	2.38±0.11^e
D1	16.82±0.82^b	1.87±0.05^a	2.50±0.23^bc	3.01±0.27^d
D2	15.92±0.65^bcd	1.77±0.06^b	2.41±0.15^bcd	2.99±0.26^d
D3	11.00±0.59^f	1.73±0.03^bc	2.17±0.13^d	2.97±0.12^d

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