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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (4): 574-583.doi: 10.7525/j.issn.1673-5102.2022.04.007

• Systematic and Evolutionary • Previous Articles     Next Articles

Phylogeography of Medicinal and Edible Homologous Plant Allium macrostemon

Tian SHI, Zhongmei MO, Min WU, Cai ZHAO()   

  1. Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering(CICMEAB),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025
  • Received:2021-03-20 Online:2022-07-20 Published:2022-07-15
  • Contact: Cai ZHAO E-mail:zhaocai_11@163.com
  • About author:SHI Tian(1995—),female,master candidates,engaged in research of plant systems and evolutionary biology.
  • Supported by:
    Guizhou science and technology support project([2019]2451-2);Guizhou biology domestic first-class construction discipline opening fund(GNYL[2017]009);Key Laboratory Opening Project of Education Department of Guizhou Province ministry of Education(KY[2019]033);National Key R&D Program of China(2016YFC0502604);Construction of modern industrial technology system for Chinese medicinal materials in Guizhou Province(GZCYTX-02)


In order to reveal the distribution pattern of genetic variation and haplotype geographical distribution pattern of Allium macrostemon, and to infer the refugia in the quaternary glaciation, the chloroplast gene fragments(psbA-trnH, rps16 and trnL-F) and nuclear gene fragments(ITS) of A. macrostemon in 14 regions of China were analyzed respectively. The results showed that the genetic diversity of A. macrostemon chloroplast gene(cpDNA) was lower than that of nuclear gene(nrDNA) (cpDNA:HT=0.868; nrDNA:HT=0.890). The AMOVA analysis of cpDNA and nrDNA showed that the genetic variations occurred mainly among populations (cpDNA:92.84%; nrDNA:98.40%), there was significant genetic differentiation among populations(cpDNA:Nst=0.918,Gst=0.866,Fst=0.928;nrDNA:Nst=0.984,Gst=0.855,Fst=0.984), and had an obvious phylogeographical structure in A. macrostemon populations(Nst>Gst). A total of 11 chloroplast and 14 nuclear haplotypes were identified in the A. macrostemon populations. The haplotype network and geographical distribution map showed that the chloroplast haplotype H3 and nuclear DNA genotype H1 had the highest frequency and were located in the center of the network structure, which might be the ancient haplotype. In addition, the glacial refuge hypothesis suggested that the region with high genetic diversity, ancient haplotype and more endemic haplotype might be the glacial refugia of A. macrostemon. Therefore, it was speculated that in the quaternary glaciation, A. macrostemon might be multiple glacial refuges in Dapanshan, Tianshui and Tonghua. The analysis could provide references for the evolution of similar herbs and enrich our understanding of the molecular phylogeography of herbaceous plants in East Asia.

Key words: Allium macrostemon, population, phylogeography, genetic variation, glacial refugia

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