Effects of Drought Stress on Leaf Structure of Eucommia ulmoides

doi:10.7525/j.issn.1673-5102.2021.06.013

Abstract

Abstract:

To explore the effects of drought stress on leaf structure of Eucommia ulmoides and understand the characteristics of drought adaptation， 2-year-old cutting seedlings of ‘Huazhong No.6’ were used as experimental materials. The results showed the leaf relative water content， sponge tissue thickness and lower epidermis thickness were significantly lower on the 16th day of drought stress compared with the control group respectively. During the drought stress， the stomatal density of leaves increased at first and then decreased， while the stomatal size expressed by stomatal area， stomatal length and stomatal width pointed a trend of “increase-decrease-increase” and there was a significant negative linear correlation among stomatal length and width and stomatal density. The main reason of the cutting seedling thickness thinning of Eucommia ulmoides was that the sponge tissue thickness became smaller. Under drought stress for 11 days， the stomata of leaves were small and dense， the stomata closure increased， while the stomata showed larger and sparser adaptation characteristics for 16 days under drought stress.

Key words: Eucommia ulmoides, drought stress, leaf structure, stomata

CLC Number:

S723.31

Feng HE, Hong-Yan DU, Pan-Feng LIU, Lu WANG, Jun QING, Lan-Ying DU. Effects of Drought Stress on Leaf Structure of Eucommia ulmoides[J]. Bulletin of Botanical Research, 2021, 41(6): 947-956.

Figures/Tables 8

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干旱胁迫时间 Drought stressing time（d）	上表皮厚度 Upper epidermis thickness（μm）		下表皮厚度 Lower epidermis thickness（μm）		栅栏组织厚度 Palisade tissue thickness（μm）		海绵组织厚度 Spongy tissue thickness（μm）		栅海比 Ratio of palisadetissue and spongy tissue
干旱胁迫时间 Drought stressing time（d）	对照 Control	干旱 Drought	对照 Control	干旱 Drought	对照 Control	干旱 Drought	对照 Control	干旱 Drought	对照 Control	干旱 Drought
6	14.44±0.79a	14.41±0.74a	12.72±0.64a	12.59±0.93a	54.19±3.18a	54.20±2.39a	85.28±1.61a	85.13±2.33a	0.64±0.03a	0.64±0.03a
11	14.48±0.70a	14.46±0.80a	12.64±0.99a	12.62±1.06a	54.26±2.48a	54.28±2.67a	85.20±1.16a	84.45±2.30a	0.64±0.04a	0.64±0.03a
16	14.43±0.79a	14.47±0.85a	12.72±1.09a	9.28±1.19b	54.36±1.85a	54.26±2.52a	85.54±1.92a	50.76±1.53b	0.64±0.03a	1.07±0.08b

干旱胁迫时间 Drought stress time（d）	气孔面积 Stomatal area （μm²）		气孔长度 Length of stomatal aperture（μm）		气孔宽度 Width of stomatal aperture（μm）		气孔密度 Stomatal aperture （No.·mm^-2）		气孔闭合百分比 Percentage of stomatal closure（%）
干旱胁迫时间 Drought stress time（d）	对照 Control	干旱 Drought	对照 Control	干旱 Drought	对照 Control	干旱 Drought	对照 Control	干旱 Drought	对照 Control	干旱 Drought
6	11.95±0.02a	19.35±2.70b	5.16±0.03a	7.96±0.92b	1.59±0.06a	2.32±0.18b	214.13±25.11a	207.28±42.25a	49.1±4.25a	35.73±4.21b
11	27.26±1.64a	7.89±0.82b	11.62±0.84a	5.38±0.17b	4.27±0.04a	1.66±0.09b	202.98±29.86a	308.03±7.27b	4.29±4.35a	42.52±1.07b
16	16.79±0.99a	14.53±1.16a	8.60±0.19a	8.07±0.65a	2.79±0.07a	2.64±0.09a	210.77±43.67a	210.74±30.08a	16.31±3.75a	27.63±4.03b

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