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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (2): 205-212.doi: 10.7525/j.issn.1673-5102.2021.02.008

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Photosynthetic and Stomatal Characteristics of Pinus koraiensis and P.sibirica under Low Temperature Stress

Fang WANG1, Zhi-Min LU1, Jun WANG1, Shi-Kai ZHANG2, Yu-Xi LI2, Shao-Chen LI1, Jian-Qiu ZHANG1, Yu-Chun YANG1()   

  1. 1.Jinlin Provincial Academy of Forestry Sciences,Changchun 130000
    2.State Key Laboratory of Northeast Forestry University,Harbin 150040
  • Received:2019-11-25 Online:2021-03-20 Published:2021-01-05
  • Contact: Yu-Chun YANG E-mail:yang-yu-chun@163.com
  • About author:WANG Fang(1988—),female,doctor,mainly engaged in forest tree genetics and breeding research.
  • Supported by:
    Opening Project of State Key Laboratory of Tree Genetics and Breeding(K2019103)


Both Pinus koraiensis and P.sibirica with strong cold resistance are famous forest species in the cold temperate zone. Compared with P.koraiensis P.sibirica has stronger cold resistance. In order to explore the physiological response and cold resistance mechanism of the two species under low temperature stress,5-year-old P.koraiensis and P.sibirica seedlings were used to be conducted by low temperature treatment. By setting three stress temperatures(0℃, -20℃ and -40℃) and three stress times(6, 24 and 48 h), taking 20℃ as control, which were used to study the photosynthetic characteristics and stomatal characteristics of P.koraiensis and P. sibirica under low temperature stress. By t test and variance analysis, there were significant differences (P<0.05) between P.koraiensis and P.sibirica for each photosynthetic index and stomatal density, both low temperature and low temperature stress time had an extremely significant effect on each photosynthetic index of P.koraiensis and P.sibiricaP<0.01), and low temperature also had an extremely significant effect on stomatal opening size and stomatal area of P.koraiensis and P.sibiricaP<0.01). The net photosynthetic rate, stomatal conductance and transpiration rate in P.koraiensis were significantly higher than those in P.sibirica at 20℃(without stress) and 0℃(low temperature stress). However, under -20℃ for 6 h, each photosynthetic index in P.sibirica was higher than that in P.koraiensis. With the decrease of temperature and the extension of stress time, each photosynthetic index of the two species showed a decreasing trend. The stomatal density in P.koraiensis was significantly higher than that in P.sibirica. Before stress(20℃), the stomas of P.koraiensis and P.sibirica were all oval. The stomatal opening size and stomatal area of the two species decreased significantly with the decrease of temperature.

Key words: Pinus koraiensis, Pinus sibirica, photosynthesis, stoma, cold resistance

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