Abstract:

The seedlings of wheat were exposed to He-Ne laser irradiation after enhanced UV-B radiated,in order to determine some physiological traits in the damage induced by UV-B radiation. The seeds were cultivated in Petridishes in a constant temperature incubator(25℃)until they were germinated.The germinated seeds were exposed to 10.08 kJ·m^-2·d^-1 UV-B radiation, one by one, and then they were irradiated by He-Ne laser with 5 mW·mm^-2 Power density.Changes about the content of free proline(Pro) and the activity of the peroxidase(POD) and polyamine oxidase(PAO)were measured to test the repair role of laser irradiation. The results showed that the damage of wheat seedlings induced by enhanced UV-B radiation in physiology can be repaired partly by He-Ne laser irradiation with decreasing the content of free Pro and raising the activity of POD and PAO. This is the first investigation reporting the use of laser irradiation treatment to repair the wheat damage induced by UV-B radiation in peroxidase and polyamine oxidaset. It implied that the changes of PAO activity have the relativity with POD in some extent.

Key words: wheat, He-Ne laser, UV-B, PAO activity, POD activity, Pro