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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (1): 79-84.doi: 10.7525/j.issn.1673-5102.2020.01.012

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Identification of Key Amino Acid Sites in AtGDPD-Like3 with Role in Arabidopsis Root Hairs

WANG Shuang, CHENG Yu-Xiang, XIA De-An   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-04-24 Online:2020-01-05 Published:2020-02-21
  • Supported by:
    Natural Scientific Foundation of China(31570580)

Abstract: AtGDPD-Like3 encode glycerophosphodiester phosphodiesterase-like(GDPD) proteins and shv3, its family gene AtGDPD-Like3 mutant, has serious root hair defects. To identify key amino acid sites in AtGDPD-Like3, we constructed S538A, V556A and D628A single site mutation of AtGDPD-Like3, which were expressed in atgdpdl3 mutants, respectively. The results showed that AtGDPD-Like3 with V556A or D628A mutation could completely restore root hairs growth defects of atgdpdl3, whereas, AtGDPD-Like3 with S538A mutation partially restored root hairs growth defects. These indicate that Ser538 is the key amino acid site in AtGDPD-Like3, which affects function of its protein. Meanwhile, it implies other key amino acid sites in AtGDPD-Like3. The results of this study would lay a foundation for further exploring the functional mechanism of AtGDPD-Like3 protein.

Key words: Arabidopsis thaliana, AtGDPD-Like3, key amino acid locus, point mutation

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