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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (5): 653-663.doi: 10.7525/j.issn.1673-5102.2019.05.003

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Chloroplast Genome Characteristics of Endangered Relict Plant Tetraena mongolica in the Arid Region of Northwest China

DUAN Yi-Zhong1, DU Zhong-Yu2,3, WANG Hai-Tao1   

  1. 1. Shaanxi Key Laboratory of Ecological Restoration in Northern Shaanxi Mining Area, Yulin University, Yulin 719000;
    2. Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021;
    3. Ministry of Education Key Laboratory for Restoration and Reconstruction of Degraded Ecosystem in Northwest China, Ningxia University, Yinchuan 750021
  • Received:2019-02-26 Online:2019-09-05 Published:2019-07-16
  • Supported by:
    National Natural Science Foundation of China(41601059)

Abstract: Tetraena mongolica is a strong dry shrub of Zygophyllaceae plant, which is unique species in China. Because it's an ancient origin and strong resistance species, it can be an ideal target for the study of biodiversity origin, environmental evolution, and academics research value as usual. Illumina Double-end Sequencing Technology was used to sequence and analyzed the chloroplast genome of T.mongolica. A total of 30 species of chloroplast genomes were selected from the order of the genus and geranium, the phylogenetic relationship with T.mongolica was carried out. The chloroplast genome of T.mongolica was 106 259 bp in length, there were 7 genes in the inverted repeat region(in the IR region), including 4 PCG genes and 3 tRNA genes. Total chloroplast genome coding 98 genes, including 65 protein-coding genes, 29 tRNA genes and 4 rRNA genes. Bioinformatics showed that 92 SSR loci were found in T.mongolica, including 74 single nucleotide repeat motifs, 7 dinucleotide repeat motifs, 1 trinucleotide repeat motif, 9 tetranucleotide repeat motifs and 1 pentanucleotide motif. There is no hexanucleotides and no single nucleotide glycosylate and the proportion of chloroplast genome SSR in T.mongolica was 80.1%. The chloroplast genome of 31 species including T.mongolica was clustered by MEGA software using neighbor-joining(NJ). The Larrea tridentate is the closest species, followed by the genus Viviania marifolia, which is closely related to the Pelargonium fulgidum. The genus Hypseocharis bilobata has the closest relationship, which indicates that T.mongolica belongs to the genus Polygonaceae, which has certain reference value for the study of T.mongolica.

Key words: Tetraena mongolica, chloroplast genome, high-throughput sequencing, phylogenetic relationship

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