Abstract: With the hypocotyls and seedling rhizomes of Rumex gmelini Turcz. as explants, the tissue culture of R.gmelini was studied by adding different concentrations of 6-BA and 2,4-D into MS basic mediums. The results showed that the better materials for multiple shoot clumps induction were seedling rhizomes; the best medium for multiple shoot clumps induction was MS+6-BA 3.0 mg·L^-1+2,4-D 0.1 mg·L^-1, the induction rate was 81.2%; the rooting medium was MS basic mediums without hormones, the rooting rate could get 92%, and under this conditions the plant regeneration could be obtained rapidly. After transplantation, about 80% of these seedlings could survive.

Key words: Rumex gmelini Turcz, tissue culture, plant regeneration