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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (3): 422-432.doi: 10.7525/j.issn.1673-5102.2018.03.015

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Screening and Identification of the Proteins Related to Drought Response in Leaves of Eucommia ulmoides

ZHAO Xin1, BAI Wei2   

  1. 1. College of Medicine, Shaanxi Institute of International Trade & Commerce, Xi'an 712046;
    2. Xi'an United Innovation Bio-pharmaceutical Incubator Co., Ltd., Xi'an 710065
  • Received:2017-11-20 Online:2018-05-15 Published:2018-05-17
  • Supported by:
    The correlation study between Danhong injection and Hcy, Epo and HIF-1α in blood plasma from rats with secondary polycythemia, Funded by Shaanxi Provincial Department of Education(14JK2017); Extraction process of Eucommia ulmoides Oliver oil through the combine method, Funded by College of Medicine, Shaanxi Institute of International Trade & Commerce(SMXY201634)

Abstract: To uncover the underlying mechanism in leaves of Eucommia ulmoides in response to drought stress, we studied the physiological response characteristic in the leaves at the drought treatment time of 0, 3, 6, 9, 12, 15 d and 2 d of recovery by pot test. We used differential proteomic analysis between the leaves of 0 and 15 d to reveal the molecular mechanism. The results showed that with the stress time prolonged, the water saturation deficit was increased. While the photosynthesis rate, transpiration rate, intercellular CO2 concentration and stomatal conductance were decreased. The activity of SOD, POD and CAT were firstly increased, then decreased. The content of MDA was firstly increased, then decreased, thereafter, increased again at the day of 15. The change profile of proline and soluble sugar content were in consistence with the change of SOD activity. After rehydration, all the parameters recovered to a certain level, but still could not recover to the control level. The total proteins extracted from the leaves that were collected at 0 and 15 d were separated by 2-DE, and the differentially expressed proteins were identified by MALDI-TOF-TOF analysis. About 36 protein spots were successfully identified, including 22 up-regulated and 14 down-regulated proteins. Further analysis of these differential expressed proteins revealed that these proteins were mainly involved in signal transduction, photosynthesis, carbon metabolism, energy metabolism, secondary metabolites synthesis, antioxidant protective enzyme, amino acid metabolism and protein metabolism. It was speculated that Eucommia ulmoides leaves firstly sensed the stress signal in response to drought, then affected photosynthesis, secondary metabolites synthesis and protein synthesis. Meanwhile, the excess ROS were eliminated by antioxidant protective enzyme. On the other hand, the glycolysis and pentose phosphate pathway were enhanced to produce the energy to supply demands of plant growth under drought stress. The physiological analysis results revealed that the proline and soluble sugar content were increased to reduce the water loss by decreasing the osmotic potential in plant cells. These results were in accordance with the change profiles of amino acid metabolism and sugar metabolism associated proteins.

Key words: Eucommia ulmoides, leaves, drought stress, proteomics

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