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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (5): 709-714.doi: 10.7525/j.issn.1673-5102.2017.05.010

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Genetic Diversity between Island and Mainland Natural Populations of Polygonatum odoratum in Dalian Area by ISSR

HAO Jiu-Cheng, JIA Xin, MU Xiao-Hong, ZHANG Heng-Qing   

  1. College of Life Sciences, Liaoning Normal University, Dalian 116081
  • Received:2017-03-04 Online:2017-09-15 Published:2017-09-15

Abstract: With Polygonatum odoratum, a plant on both the mainland and islands in Dalian, we studied the genetic diversity of 262 P.odoratum from 5 islands and 4 mainland populations by ISSR molecular marker technology. Information of 120 loci was obtained by amplification of 10 ISSR primers that were screened out, where the percentage of polymorphic bands was 91.67%, the gene diversity index of Nei's was 0.346 0, and Shannon information index was 0.510 8. Its genetic differentiation coefficient was 0.117 4, and its gene flow was 3.758 5. The genetic diversity of natural populations of P.odoratum was relatively rich with relatively frequent gene exchange among different populations, and its genetic distance had certain correlation with its geographic distance. By comparison between genetic diversity of populations from the mainland and islands, the genetic diversity of populations from islands was slightly higher than that from the mainland, indicating that under a more isolated ecological environment and more complicated selection pressure, populations of P.odoratum on islands might accumulate more genetic variation to form high levels of genetic diversity. Our results would provide evidence for further discussion on laws of genetic evolution of populations of natural plants in isolated ecological environments.

Key words: ISS-PCR, Polygonatum odoratum, genetic diversity, genetic structure

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