Abstract: The explants of Chinese magnoliavine tender stems with axillary buds are cultured in MS medium with addition of different concentration and kinds of hormone. The results show that sprouting of axillary bud is best on MS medium with addition of 6-BA_2.0+ZT_0.1 mg/L;and the multiplication of adventitious bud is best on MS medium with the addition of 6-BA_2.0+NAA_0.2+ZT_0.1 mg/L;and the growth of roots is best on MS medium with addition of NAA_0.2 mg/L. Seedlings with growing to 2.0~2.5 cm and 3~5 roots are transplanted to the pots with disinfect soil after 2 days training. 10 days later, the survival rate reaches 100%.

Key words: Explant, Chinese magnoliavine, Tissue culture