ISSR Analysis on the Genetic Diversity of Relic Plant Bretschneidera sinensis from Wuyishan Region

doi:10.7525/j.issn.1673-5102.2015.04.014

Abstract

Abstract: Bretschneidera sinensis Hemsl., with important research value, is an endangered species endemic in East and South Chin. We used inter-simple sequence repeat(ISSR) markers studied the genetic diversity within and among six populations of B.sinensis. The genetic diversity at species level was high(PPB: 75.70%; H_ES: 0.304 5; H: 0.450 1). However, relatively low genetic diversity existed within populations. Population in Minjiangyuan(MJY) exhibited the greatest level of variability(PPB: 60.13%; H_EP: 0.238 1; H: 0.347 5), whereas the population in Baishanzu(BSZ) showed its own variability at the lowest level(PPB: 44.86%; H_E: 0.173 6; H: 0.255 6). By the genetic analysis of the ISSRs, we revealed a relatively high level of genetic differentiation among populations by Nei’s gene diversity statistics(21.81%), Shannon’s information measure(22.79%) and analysis of molecular variance(AMOVA)(18.62%). The main factors responsible for the relatively high level of differentiation among populations were probably related to the predominant outcrossing reproductive system and recent habitat fragmentation, and a significant correlation was between genetic distance and geographic distance(r=0.626 7, P<0.05). The high diversity would hold promise for conservation and restoration efforts to save the endangered species from extinction.

Key words: Bretschneidera sinensis, inter-simple sequence repeats(ISSR), genetic diversity, genetic structure

CLC Number:

LIN Le-Jing1,2；JIN Chu-Qi1；ZHENG Chun-Hao3；ZHOU Ya-Mei1；ZHOU Ying1. ISSR Analysis on the Genetic Diversity of Relic Plant Bretschneidera sinensis from Wuyishan Region[J]. Bulletin of Botanical Research, 2015, 35(4): 559-563.

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