Abstract: We studied the tissue culture system of Acacia crassicarpa by using the stemsegment with buds collected from 16-years plant as explants. The buds could be induced successfully on 2% of MS+sucrose with 67.33% of the survival rate and 80.56% of shooting rate after sterilized with 75% of alcoho1 and 0.1% of mercuric chloride for 30 s and 12 min, respectively. Low concentration of activated carbon effectively alleviated the verification, the best medium for multiplication was Modified-MS+6-BA 0.5 mg·L^-1+NAA 0.25 mg·L^-1+Ac 0.1 g·L^-1+sucrose 3% and the multiplication number was 3.72 after 35 days. The best medium for rooting was Modified-MS+IBA 0.5 mg·L^-1+2% of sucrose, and the rooting rate was 98.83%. The survival rate reached 90.00% after transplanting seedlings to the nutrition cup with yellow soil.

Key words: Acacia crassicarpa, axillary, tissue culture, verification