Ultrasonic-assisted Extraction of Total Flavonoids from Peony Tree Seed Coat and Its Anticoagulation Activity

doi:10.7525/j.issn.1673-5102.2014.06.024

Abstract

Abstract: We used ultrasonic-assisted extraction method to extract total flavonoids from seed coat of peony tree, and studied the extraction conditions including volume fraction of ethanol, ratio of liquid to solid (mL·g^-1), ultrasonic time and power which influenced the extraction yield of total flavonoids. The results of orthogonal test indicated the optimum extraction conditions of total flavonoids of seed coat were: volume fraction of 55% as solvent, liquid to solid ratio of 20∶1, ultrasonic time of 40 min and ultrasonic power of 140 W. Under these conditions, the total flavonoids yield of seed coat was 46.50‰. After column chromatography for flavonoids purification of seed coat extract, it contained 203.5 mg·g^-1 quercetin and 23.2 mg·g^-1 luteolin. Seed coat flavonoids of peony tree showed high activity of anticoagulation activity in experiments of in vivo rat.

Key words: ultrasonic-assisted extraction, seed coat of peony tree, flavonoid, anticoagulation

CLC Number:

WANG Hong-Zheng1,2；LIU Wei1,2；ZHANG Lin1,2；CONG Ying1,2；ZAN Peng1,2；WANG Hua1,2,3；ZU Yuan-Gang1,2；CHEN Hua-Feng1,2*；ZHAO Xiu-Hua1,2. Ultrasonic-assisted Extraction of Total Flavonoids from Peony Tree Seed Coat and Its Anticoagulation Activity[J]. Bulletin of Botanical Research, 2014, 34(6): 856-860.

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