Abstract: The pollen viability of Anemone obtusiloba with three-olor flowers (yellow, pale yellow and white) was detected with four methods: TTC dyeing, I2-I dyeing, inorganic acid test, sucrose-ased germination, the determination effects of different methods were compared, and the dynamic change of pollen viability within a day was understood. The results show that: (1) The fertile rate of pollen of A.obtusiloba is high, about 80%. Pollens diameter are 28.75-33.75 m. (2) The concentration of TTC can influence the determination of pollen vitality, when the concentration was 0.5%, it was better than the others. (3) I2-I dyeing is not ideal, dyeing rate is only 8.33%-22.2%, and there is no significant difference within three-olor flowers. (4) The pollen vitality of A.obtusiloba with three-olor flowers have differences, with yellow the highest, followed by light yellow, white the minimum. TTC dyeing is a simple and rapid determination method of A.obtusiloba. Within a day, pollen vitality is maximum from 12:00-14:00 pm.

Key words: Anemone obtusiloba, detection methods, fertility, pollen, viability