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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (2): 288-299.doi: 10.7525/j.issn.1673-5102.2023.02.014

• Molecular biology • Previous Articles     Next Articles

Function Analysis of the Transcription Factor PsnbZIP1 of Populus simonii×P. nigra in Response to Salt Stress

Shixian LIAO, Yuting WANG, Liben DONG, Yongmei GU, Fenglin JIA, Tingbo JIANG, Boru ZHOU()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2022-05-18 Online:2023-03-20 Published:2023-03-07
  • Contact: Boru ZHOU E-mail:zhouboru2020@nefu.edu.cn
  • About author:LIAO Shixian(1998—),male,postgraduate student,major in the field of forestry functional genome.
  • Supported by:
    Application Technology Research and Development Program of Heilongjiang Province(GA20B401)


To reveal the function of transcription factor PsnbZIP1 from Populus simonii × P. nigra under abiotic stresses, P.simonii × P. nigra was used as material, and the PsnbZIP1 transcription factor gene was cloned with an ORF length of 432 bp, and the molecular mechanism of PsnbZIP1 under salt stress was analyzed. The expression pattern of PsnbZIP1 in P. simonii × P. nigra was analyzed under 150 mmol·L-1 NaCl by q-PCR, it was found that the expression of the gene increased rapidly. The PsnbZIP1 was predicted to be a hydrophilic unstable protein without trans-membrane structure but with signal peptide by bioinformatics analysis. The sub-cellular localization of the gene was observed by Agrobacterium mediated transient expression in tobacco. The results showed that the gene was a nuclear localization protein. It was confirmed by Y2HGold yeast competent cells that the gene had no transcriptional activation activity in yeast. The analysis of the promoter sequence of the PsnbZIP1 gene showed that the promoter contained auxin response elements, ABA response elements, light response elements and seed-specific cis-acting regulatory elements respectively, which might play a role in plant growth and development and response to stress.The promoter also included the MYB binding site and MYBHv1 binding site involved in drought induction, indicating that the gene was likely to interact with some of MYB involved in drought induction.

Key words: Populus simonii × P.nigra, bZIPtranscription factor, salt stress, bioinformatics analysis

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