Effects of N addition on Root Exudates and Their Mediated Nutrient Rransformation Processes in a Betula albosinensis Burk Forest in Southwest China

doi:10.7525/j.issn.1673-5102.2023.02.005

Abstract

Abstract:

At present， we still lack a deep understanding of the response direction and amplitude of root exudate flux and related ecological consequences to different nitrogen（N） deposition levels. In order to reveal the difference in the effects of different N addition treatments on root exudate C flux and its mediated soil nutrient transformation process， the typical Betula albosinensis Burk forest in the mountainous area of southwest China was used as materials， and different nitrogen deposition levels were simulated by in-situ N addition experiment（control group， 0 kg·hm^-2?a^-1）； low nitrogen treatment， 25 kg·hm^-2?a^-1； High nitrogen treatment， 50 kg·hm^-2?a^-1 respectively. The results showed that：（1）N addition significantly reduced the C input rate（the average root exudation rate per unit root biomass decreased by 14.87% under low nitrogen（N25））and annual C input flux of root exudates（about 45.01% reduction under low nitrogen condition）（P<0.05）， and high N treatment had stronger inhibition effect on root exudates C input. （2）N addition significantly inhibited soil N mineralization rate and related microbial extracellular enzyme activities（P<0.05）， and significantly reduced its rhizosphere effect. In conclusion， N deposition significantly inhibited the root exudates C flux and its mediated soil nutrient transformation process， and this inhibition effect increased with the increase of N deposition level. The results could enrich the understanding of carbon-nutrient cycling in forest under global climate change.

Key words: Betula albosinensis Burk forest, root exudates, soil N transformation, rhizosphere effect, nitrogen deposition

CLC Number:

Q180.44

Zhenggang XU, Hang XU, Xiutao PENG, Shijun LIANG, Rong LIU, Shanghua LUO, Juan XIAO. Effects of N addition on Root Exudates and Their Mediated Nutrient Rransformation Processes in a Betula albosinensis Burk Forest in Southwest China[J]. Bulletin of Botanical Research, 2023, 43(2): 194-206.

Figures/Tables 11

Fig.1

Table 1

Table 2

Fig.2

Table 3

Fig.3

Table 4

Table 5

Fig.4

Fig.5

Fig.6

References 47

1	尹华军，张子良，刘庆.森林根系分泌物生态学研究：问题与展望［J］.植物生态学报，2018，42（11）：1055-1070.
	YIN H J， ZHANG Z L， LIU Q.Root exudates and their ecological consequences in forest ecosystems：problems and perspective［J］.Chinese Journal of Plant Ecology，2018，42（11）：1055-1070.
2	张福锁，申建波.根际微生态系统理论框架的初步构建［J］.中国农业科技导报，1999，1（4）：15-20.
	ZHANG F S， SHEN J B.Preliminary development of the theoretical concept on rhizosphere micro-ecosystem［J］.Review of China Agricultural Science and Technology，1999，1（4）：15-20.
3	OBURGER E， JONES D L.Sampling root exudates - mission impossible？［J］.Rhizosphere，2018，6：116-133.
4	吴林坤，林向民，林文雄.根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望［J］.植物生态学报，2014，38（3）：298-310.
	WU L K， LIN X M， LIN W X.Advances and perspective in research on plant-soil-microbe interactions mediated by root exudates［J］.Chinese Journal of Plant Ecology，2014，38（3）：298-310.
5	FINZI A C， ABRAMOFF R Z， SPILLER K S，et al.Rhizosphere processes are quantitatively important components of terrestrial carbon and nutrient cycles［J］.Global Change Biology，2015，21（5）：2082-2094.
6	PHILLIPS R P， FAHEY T J.The influence of soil fertility on rhizosphere effects in northern hardwood forest soils［J］.Soil Science Society of America Journal，2008，72（2）：453-461.
7	ZHANG S Z， LI Y H， KONG C H，et al.Interference of allelopathic wheat with different weeds［J］.Pest Management Science，2016，72（1）：172-178.
8	HAICHAR F E Z， SANTAELLA C， HEULIN T，et al.Root exudates mediated interactions belowground［J］.Soil Biology and Biochemistry，2014，77：69-80.
9	YU G R， JIA Y L， HE N P，et al.Stabilization of atmospheric nitrogen deposition in China over the past decade［J］.Nature Geoscience，2019，12（6）：424-429.
10	REAY D S， DENTENER F， SMITH P，et al.Global nitrogen deposition and carbon sinks［J］.Nature Geoscience，2008，1（7）：430-437.
11	VAN GROENIGEN J W， VAN KESSEL C， HUNGATE B A，et al.Sequestering soil organic carbon：a nitrogen dilemma［J］.Environmental Science & Technology，2017，51（9）：4738-4739.
12	BRZOSTEK E R， DRAGONI D， BROWN Z A，et al.Mycorrhizal type determines the magnitude and direction of root-induced changes in decomposition in a temperate forest［J］.New Phytologist，2015，206（4）：1274-1282.
13	KLEIN T， SIEGWOLF R T W， KÖRNER C.Belowground carbon trade among tall trees in a temperate forest［J］.Science，2016，352（6283）：342-344.
14	LALIBERTÉ E.Below-ground frontiers in trait-based plant ecology［J］.New Phytologist，2017，213（4）：1597-1603.
15	尹华军，刘庆.西南亚高山森林根系分泌物生态学研究［M］.北京：科学出版社，2019：36-44.
	YIN H J， LIU Q.Ecological studies on root exudation of subalpine forests in Southwest China［M］.Beijing：Science Press，2019：36-44.
16	白志勇，任可心，陈梦雨，等.N沉降下川中丘陵地区柏树人工林根系分泌物特征及其介导的根际C、N转化过程［J］.生态学杂志，2022，41（3）：454-464.
	BAI Z Y， REN K X， CHEN M Y，et al.Root exudates characteristics and associated soil C and N transformation processes of Cupressus funebris plantation in hilly region of central Sichuan under N deposition［J］.Chinese Journal of Ecology，2022，41（3）：454-464.
17	马俊，党坤良，王连贺，等.秦岭火地塘林区红桦林生物量和蓄积量变化研究［J］.西北林学院学报，2016，31（3）：204-210.
	MA J， DANG K L， WANG L H，et al.Research on the changes in biomass and volume of Betula albo-sinensis forest at Huoditang region［J］.Journal of Northwest Forestry University，2016，31（3）：204-210.
18	郭垚鑫，康冰，李刚，等.小陇山红桦次生林物种组成与立木的点格局分析［J］.应用生态学报，2011，22（10）：2574-2580.
	GUO Y X， KANG B， LI G，et al.Species composition and point pattern analysis of standing trees in secondary Betula albo-sinensis forest in Xiaolongshan of west Qinling Mountains［J］.Chinese Journal of Applied Ecology，2011，22（10）：2574-2580.
19	张泽钧.四川唐家河国家级自然保护区生物多样性研究［M］.北京：科学出版社，2016：240-245.
	ZHANG Z J.Research on biodiversity in Tangjiahe national nature reserve，China［M］.Beijing：Science Press，2016：240-245.
20	王超，尤海舟，毕君.小五台山自然保护区红桦林的群落结构与演替［J］.中南林业科技大学学报，2017，37（5）：69-73.
	WANG C， YOU H Z， BI J.The community structure and succession of Betula ablo-sinensis forest in Xiaowutai Mountain Nature Reserve［J］.Journal of Central South University of Forestry & Technology，2017，37（5）：69-73.
21	殷博，董鹏飞，党坤良.抚育间伐对红桦林生态系统碳密度的影响［J］.西北林学院学报，2019，34（1）：105-112.
	YIN B， DONG P F， DANG K L.Effects of thinning on carbon density of Betula albo-sinensis ecosystem［J］.Journal of Northwest Forestry University，2019，34（1）：105-112.
22	胡锦矗.四川唐家河自然保护区综合科学考察报告［M］.成都：四川科学技术出版社，2005：12-14.
	HU J C.Report of the comprehensive survey on Tangjiahe Nature Reserve in Sichuan，China［M］.Chengdu：Sichuan Science and Technology Press，2005：12-14.
23	孙誉育，唐波，尹春英，等.水氮耦合效应对红桦幼苗生长的影响及其生理机制［J］.应用与环境生物学报，2015，21（4）：710-716.
	SUN Y Y， TANG B， YIN C Y，et al.Effects of water and nitrogen coupling on growth of Betual albo-sinensis seedlings and its physiological mechanism［J］.Chinese Journal of Applied and Environmental Biology，2015，21（4）：710-716.
24	魏俊.四川唐家河国家级自然保护区药用植物资源多样性特征分析［D］.雅安：四川农业大学，2018.
	WEI J.Analysis on medicinal plant resources diversity characteristics in the Tangjiahe National Nature Reserve，Sichuan Province［D］.Yaan：Sichuan Agricultural University，2018.
25	尹明珍，郭婉玑，朱晓敏，等.氮沉降下西南亚高山针叶林生长的养分限制特征［J］.应用与环境生物学报，2021，27（1）：1-7.
	YIN M Z， GUO W J， ZHU X M，et al.Nutrient limiting characteristics of subalpine coniferous forests under conditions of nitrogen deposition in the southwest mountains of China［J］.Chinese Journal of Applied and Environmental Biology，2021，27（1）：1-7.
26	刘庆，肖娟，李宇飞，等.一种根系分泌物原位收集装置及系统：CN202471455U［P］.2012-10-03.
	LIU Q， XIAO J， LI Y F，et al.Root system secretion normal position collecting device and system：CN202471455U［P］.2012-10-03.
27	何为，杨雪英，肖娟，等.氮素富集对青藏高原东缘窄叶鲜卑花灌丛根系分泌物碳输入的影响［J］.植物生态学报，2017，41（6）：610-621.
	HE W， YANG X Y， XIAO J，et al.Effects of nitrogen enrichment on root exudative carbon inputs in Sibiraea angustata shrubbery at the eastern fringe of Qinghai-Xizang Plateau［J］.Chinese Journal of Plant Ecology，2017，41（6）：610-621.
28	JIANG Z， WANG Q T， XIAO J，et al.Differential responses of N benefit mediated by root exudate inputs to N addition between two subalpine forests［J］.Rhizosphere，2021，19：100404.
29	任可心.N沉降下川中丘陵柏树人工林根系分泌物特征及其介导的C、N转化过程［D］.南充：西华师范大学，2021.
	REN K X.Characteristics of root exudates and their mediated C，N transformation of Cupressus funebris plantation in the hilly area of central Sichuan under nitrogen deposition［D］.Nanchong：China West Normal University，2021.
30	刘雅星，范广洲，朱叶玉，等.中国木本植物生长季的变化特征分析［C］//第27届中国气象学会年会现代农业气象防灾减灾与粮食安全分会场论文集.北京：中国气象学会，2010：585-591.
	LIU Y X， FAN G Z， ZHU Y Y，et al.Analysis on the variation characteristics of woody plants in China during the growing season［C］//Proceedings of the 27th Annual Meeting of the Chinese meteorological society，branch of modern agrometeorological disaster prevention and reduction and food security.Beijing：China Meteorological Society，2010：585-591.
31	VANCE E D， BROOKES P C， JENKINSON D S.An extraction method for measuring soil microbial biomass C［J］.Soil Biology and Biochemistry，1987，19（6）：703-707.
32	JOERGENSEN R G， MUELLER T.The fumigation-extraction method to estimate soil microbial biomass：calibration of the K_EN value［J］.Soil Biology and Biochemistry，1996，28（1）：33-37.
33	BRZOSTEK E R， GRECO A， DRAKE J E，et al.Root carbon inputs to the rhizosphere stimulate extracellular enzyme activity and increase nitrogen availability in temperate forest soils［J］.Biogeochemistry，2013，115（1/2/3）：65-76.
34	HUO C F， LUO Y Q， CHENG W X.Rhizosphere priming effect：a meta-analysis［J］.Soil Biology and Biochemistry，2017，111：78-84.
35	ZANG H D， BLAGODATSKAYA E， WANG J Y，et al.Nitrogen fertilization increases rhizodeposit incorporation into microbial biomass and reduces soil organic matter losses［J］.Biology and Fertility of Soils，2017，53（4）：419-429.
36	CARRARA J E， WALTER C A， HAWKINS J S，et al.Interactions among plants，bacteria，and fungi reduce extracellular enzyme activities under long-term N fertilization［J］.Global Change Biology，2018，24（6）：2721-2734.
37	KAISER C， KORANDA M， KITZLER B，et al.Belowground carbon allocation by trees drives seasonal patterns of extracellular enzyme activities by altering microbial community composition in a beech forest soil［J］.New Phytologist，2010，187（3）：843-858.
38	PHILLIPS R P， BERNHARDT E S， SCHLESINGER W H.Elevated CO₂ increases root exudation from loblolly pine（Pinus taeda） seedlings as an N-mediated response［J］.Tree Physiology，2009，29（12）：1513-1523.
39	KRAUSE K， CHERUBINI P， BUGMANN H，et al.Growth enhancement of Picea abies trees under long-term，low-dose N addition is due to morphological more than to physiological changes［J］.Tree Physiology，2012，32（12）：1471-1481.
40	FARRAR J， HAWES M， JONES D，et al.How roots control the flux of carbon to the rhizosphere［J］.Ecology，2003，84（4）：827-837.
41	马俐，周志强，王正文.刈割和氮添加对松嫩平原羊草草原碳固持的影响［J］.生态学杂志，2016，35（1）：87-94.
	MA L， ZHOU Z Q， WANG Z W.Effects of mowing and nitrogen addition on carbon sequestration of Leymus chinensis grasslands in the Songnen Plain，Northeast China［J］.Journal of Ecology，2016，35（1）：87-94.
42	ASHRAF M， SHAHZAD S M， IMTIAZ M，et al.Nitrogen nutrition and adaptation of glycophytes to saline environment：a review［J］.Archives of Agronomy and Soil Science，2018，64（9）：1181-1206.
43	王延平，王华田，谭秀梅，等.杨树人工林品种更替连作与非更替连作根际效应的比较［J］.生态学报，2010，30（5）：1379-1389.
	WANG Y P， WANG H T， TAN X M，et al.Comparison on rhizosphere effect of cultivar alternation and non-alternation continuous cropping poplar（Populus deltoids） plantation［J］.Acta Ecologica Sinica，2010，30（5）：1379-1389.
44	WANG Q T， CHEN L Y， XU H，et al.The effects of warming on root exudation and associated soil N transformation depend on soil nutrient availability［J］.Rhizosphere，2021，17：100263.
45	CHENG W X， PARTON W J， GONZALEZ-MELER M A，et al.Synthesis and modeling perspectives of rhizosphere priming［J］.New Phytologist，2014，201（1）：31-44.
46	LIN G G， GUO D L， LI L，et al.Contrasting effects of ectomycorrhizal and arbuscular mycorrhizal tropical tree species on soil nitrogen cycling：the potential mechanisms and corresponding adaptive strategies［J］.Oikos，2018，127（4）：518-530.
47	JONES D L， HODGE A， KUZYAKOV Y.Plant and mycorrhizal regulation of rhizodeposition［J］.New Phytologist，2004，163（3）：459-480.

[1]	Zhaoyi LI, Longfei HAO, Tingyan LIU, Yanhong HE, You ZHANG, Shulan BAI, Xinyu YANG. AM Fungi Inoculation on Root Morphology and Nutrient Loading of Clematis fruticosa Seedlings under Simulated Atmospheric Nitrogen Deposition [J]. Bulletin of Botanical Research, 2022, 42(5): 886-895.
[2]	Xu-Fu WANG, Long-Fei HAO, Jia-Xin HAO, Wen-Ying HAO, Hui-Ga BAO, Shu-Lan BAI. Growth Responses of Pinus sylvestris var. mongolica Seedlings under Simulated Nitrogen Deposition and Different Inoculation of Ectomycorrhizal Fungi Treatments [J]. Bulletin of Botanical Research, 2021, 41(1): 138-144.
[3]	Ting-Yan LIU, Long-Fei HAO, Xu-Fu WANG, Hai-Xia YAN, Shu-Lan BAI. Effects of Nitrogen Deposition and Ectomycorrhizal Fungi on Root Architecture and Rhizosphere Soil Enzyme Activities of Larix olgensis Seedlings [J]. Bulletin of Botanical Research, 2021, 41(1): 145-151.
[4]	ZHAO Zhe, JIN Ze-Xin. Effects of Simulated Nitrogen Deposition on the Growth and the Content of Non-structure Carbohydrate of Sinocalycanthus chinensis Seedlings [J]. Bulletin of Botanical Research, 2020, 40(1): 41-49.
[5]	MA Peng-Yu, ZHANG Hong-Guang, ZAN Peng, GU Wei-Ping, WEN Lu-Ning, ZHANG Zi-Jia, WENG Hai-Long, SUN Tao, MAO Zi-Jun. Effects of Long-term Nitrogen Addition on Soil Enzymes in Larix gmelinii Plantation in Northeast China [J]. Bulletin of Botanical Research, 2019, 39(4): 598-603.
[6]	WAN Xue-Bing, WANG Qing-Gui, YAN Guo-Yong, XING Ya-Juan. Response of Ecological Stoichiometric Characteristics and Photosynthetic Characteristics of Plant Leaves to Long-term N Deposition in Natural Secondary Forest [J]. Bulletin of Botanical Research, 2019, 39(3): 407-420.
[7]	WANG Xiao-Ping, XIAO Xiao, TANG Tian-Wen, LI Yun-Xiang, XIAO Juan. Seasonal Changes of the Input of Root Exudates and Its Driving Characteristics of Rhizosphere Microbe in a Cercidiphyllum japonicum Sieb. Plantation [J]. Bulletin of Botanical Research, 2018, 38(1): 47-55.
[8]	LI Yuan-Yuan, WANG Zheng-Wen, SUN Tao. Response of Fine Root Decomposition to Long-term Nitrogen Addition in the Temperate Forest [J]. Bulletin of Botanical Research, 2017, 37(6): 848-854.
[9]	LIU Jian-Cai;CHEN Jin-Ling;JIN Guang-Ze*. Response of Soil Organic Carbon and Nutrients to Simulated Nitrogen Deposition in Typical Mixed Broadleaved-Korean Pine Forest [J]. Bulletin of Botanical Research, 2014, 34(1): 121-130.
[10]	WANG Chuan-Hua;WANG Yuan;LI Jun-Qing. Effects of Light and Nitrogen Supply on the Carbon Balance of Seedling of Platycarya strobilacea* [J]. Bulletin of Botanical Research, 2012, 32(2): 177-182.
[11]	GUO Xiao-Rui;WANG Hong-Ling;WANG Hua-Nan;DUAN Xi-Hua. The Effects of Supplementary UV-B Radiation and Nitrogen Levels on the Growth and Physiological Metabolism of Catharanthus roseus* [J]. Bulletin of Botanical Research, 2011, 31(5): 592-596.
[12]	WANG Ying;KAISAR Sulayman;LI Jin;ZHANG Yun-Ling;ZHU Guo-Qiang;Ayibieke. Autotoxicity of Root Exudates of Fritillaria pallidiflora* Schvek [J]. Bulletin of Botanical Research, 2010, 30(2): 248-252.