Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (2): 186-193.doi: 10.7525/j.issn.1673-5102.2023.02.004

• Genetic and Breeding • Previous Articles     Next Articles

Genomic Survey Analysis of Endangered Plant Tetraena mongolica

Ziyin WANG1,2,3, Bingru LIU1,2,3(), Zihao LI1,2,3, Jifei WANG4, Pengbin YANG1   

  1. 1.College of Biological Science and Engineering,North Minzu University,Yinchuan 750021
    2.Key Laboratory of Ecological Protection of Agro-pastoral Ecotones in the Yellow River Basin,National Ethnic Affairs Commission of the People’s Republic of China,Yinchuan 750021
    3.Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments in Ningxia Province,Yinchuan 750021
    4.Ningxia Helan Mountain National Nature Reserve Administration,Yinchuan 750021
  • Received:2022-06-23 Online:2023-03-20 Published:2023-03-07
  • Contact: Bingru LIU E-mail:bingru.liu@163.com
  • About author:WANG Ziyin(1998—),male,master’s degree student,research interests:plant ecology.
  • Supported by:
    Ningxia Hui Autonomous Region Science and Technology Innovation Leaders Project(2021GKLRLX12);Scientific Research Initiation Project for the Introduction of High-level Talents of North Minzu University(2019KYQ001)


To assess the size and complexity of the genome of the endangered plant Tetraenamongolica, and reveal the hyper-arid mechanism of T.mongolica and further explore its characteristic genetic resources, and to better decipher the whole genome information of T.mongolica, the second-generation high-throughput sequencing technology of genome survey analysis was used as the method, and bioinformatics method was used to estimate the genome information such as heterozygosity, repeat sequence and GC content of T.mongolica respectively. The results showed that the genome size of T. mongolica was 1 079.25 Mb, the corrected genome size was 1 065.84 Mb, and the heterozygosity rate was 0.76%, the percentage of repetitive sequences was 75.25%, and the GC content was 33.57% respectively. After the initial assembly of T. mongolica genome 3 502 126 contigs were obtained, totaling 682 Mb, with an N50 of 187 bp. It was hypothesized that T. mongolica genome belonged to a homo-tetraploid complex genome, which was difficult to assemble by whole-genome sequencing. Due to the high heterozygosity of T. mongolica, the third-generation high-throughput sequencing technology(single-molecule sequencing) combined with chromatin region capture technology could be used subsequently, which was expected to finally obtain a high-quality whole-genome map of T. mongolica.

Key words: Tetraena mongolica, genome sequencing, survey analysis, heterozygosity rate, repeat sequence ratio

CLC Number: