Effects of NaCl Stress on Morphological and Physiological Characteristics of Magnolia denudata Desr.

doi:10.7525/j.issn.1673-5102.2021.04.015

Abstract

Abstract:

In order to explore the adaptability of Magnolia denudata Desr. to salt stress， nutrient solution was used to simulate salt stress， and the growth traits， the physiological and biochemical response characteristics were determined and analyzed under different stress levels respectively. During the whole stress process， the symptom level of salt damage was reached up to grade 2， the leaves appeared yellow and scorched. With the stress time longer， the relative electric conductivity and contents of MDA and proline in leaves increased respectively. The contents of chlorophyll b， soluble sugar， soluble protein， SOD and POD activity increased first and then decreased. The contents of chlorophyll and chlorophyll a increased first and then decreased under 100 and 200 mmol·L^-1 of NaCl stress respectively， but decreased under 300 mmol·L^-1 of NaCl stress. M. denudata had certain ability of salt tolerance， and it could be used in mild saline soil.

Key words: NaCl stress, Magnolia denudata, physiology characteristics, salt tolerance

CLC Number:

S685.15

He-Xian JIN, Xu-Yue SHEN, Rong-Rong CHEN, Zhen WU, Ya-Mei SHEN, Dong-Mei ZHANG. Effects of NaCl Stress on Morphological and Physiological Characteristics of Magnolia denudata Desr.[J]. Bulletin of Botanical Research, 2021, 41(4): 596-603.

Figures/Tables 13

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References 26

1	张浪.上海市花“白玉兰”［J］.园林，2020（5）：1.
	Zhang L.Magnolia denudata，City flower of Shanghai［J］.Landscape Architecture，2020（5）：1.
2	杨传宝.白玉兰育苗栽培技术［J］.安徽林业科技，2017，43（5）：61-63.
	Yang C B.Seeding and cultivation techniques of Magnolia denudata［J］.Anhui Forestry Science and Technology，2017，43（5）：61-63.
3	王晶，岳琳，王亚玲.中国玉兰资源及其繁育技术［J］.园林，2020（5）：12-16.
	Wang J，Yue L，Wang Y L.Magnolia denudata resources of china and breeding technologies［J］.Landscape Architecture，2020（5）：12-16.
4	郝跃，彭祚登，马履一.红花玉兰与白玉兰播种育苗效果的对比研究［J］.北方园艺，2010，（4）：101-104.
	Hao Y，Peng Z D，Ma L Y.Compare studies on the seedlings of Magnolia wufengensis and Magnolia denudata［J］.Northern Horticulture，2010，211（4）：101-104.
5	庄启茂.白玉兰容器扦插快繁技术试验［J］.防护林科技，2019，（5）：41-44.
	Zhuang Q M.Micropropagation of Magnolia denudata cuttings container［J］.Protection Forest Science and Technology，2019，（5）：41-44.
6	居萍，李良俊.七叶树和白玉兰种子休眠机理分析［J］.福建林业科技，2016，43（1）：68-72.
	Ju P，Li L J.Study on dormancy mechanism of seeds of Aesculus chinensis and Magnolia denudata［J］.Journal of Fujian Forestry Science and Technology，2016，43（1）：68-72.
7	孙双印，李晓靖.低温胁迫条件下白玉兰与紫叶李光合生理特性研究［J］.林业与生态科学，2018，33（2）：196-202.
	Sun S Y，Li X J.The effect of low temperature stress on photosynthesis in Magnolia denudata and Prunus cerasifera f. atropurpurea［J］.Forestry and Ecological Sciences，2018，33（2）：196-202.
8	常鹏杰，申亚梅，董彬，等.玉兰盐胁迫下qRT-PCR分析中内参基因的筛选［J］.农业生物技术学报，2018，26（9）：1611-1620.
	Chang P J，Shen Y M，Dong B，et al.Selection of reference gene in Magnolia denudata under salt stress using qRT-PCR ［J］.Journal of Agricultural Biotechnology，2018，26（9）：1611-1620.
9	魏凤巢，夏瑞妹，沈哲东.上海市滨海盐渍土改良技术的研究与实践［J］.中国园林，2008，24（2）：85-89.
	Wei F C，Xia R M，Shen Z D.Research and practice on amelioration technologies of coastal saline soil in Shanghai［J］.Chinese Landscape Architecture，2008，24（2）：85-89.
10	张建锋，张德顺，陈光才，等.上海滨海滩涂盐生植物分布及其生物量调查研究［J］.江西农业学报，2015，27（2）：26-29，36.
	Zhang J F，Zhang D S，Chen G C，et al.Investigation on distribution and biomass of halophytes at beach in Shanghai［J］.Acta Agriculturae Jiangxi，2015，27（2）：26-29，36.
11	李合生.植物生理生化实验原理和技术［M］.北京：高等教育出版社，2000.
	Li H S.Principles and techniques of plant physiological biochemical experiment［M］.Beijing：Higher Education Press，2000.
12	尤佳，张菁，王文瑞，等.NaCl处理下黄花补血草幼苗生理特性的变化［J］.植物研究，2013，33（1）：45-50.
	You J，Zhang J，Wang W R，et al.Changes of physiological properties in Limonium aureum（Linn．） hill seedlings under salt stress［J］.Bulletin of Botanical Research，2013，33（1）：45-50.
13	马书荣，李韫，石美玉，等.盐碱胁迫对蒙古柳无性系生理特性的影响［EB/OL］.（2020-02-27）.
	Ma S R，Li Y，Shi M Y，et al.Effects of Salix linearistipularis stress on physiological characteristics of Mongolian willow seedlings［EB/OL］.（2020-02-27）.
14	乌凤章.三个越橘品种对盐胁迫的生长和生理响应及耐盐性差异［J］.植物生理学报，2019，55（11）：1638-1646.
	Wu F Z.Growth and physiological response and salt-tolerance differences of three blueberry seedlings under salt stress［J］.Plant Physiology Journal，2019，55（11）：1638-1646.
15	于金平，俞珊，梁有旺，等.NaCl胁迫对美国白蜡幼苗部分生理指标的影响［J］.植物资源与环境学报，2014，23（1）：110-112.
	Yu J P，Yu S，Liang Y W，et al.Effect of NaCl stress on some physiological indexes of Fraxinus americana seedling［J］.Journal of Plant Resources and Environment，2014，23（1）：110-112.
16	张艳艳，刘威，宣亚楠，等.水杨酸对盐胁迫下唐古特白刺活性氧代谢和细胞膜透性抑制的缓解效应［J］.东北林业大学学报，2013，41（12）：56-59.
	Zhang Y Y，Liu W，Xuan Y N，et al.Effects of salicylic acid mitigating under salt stress on active oxygen metabolism and membrane stability in Nitratia tangutorum［J］.Journal of Northeast Forestry University，2013，41（12）：56-59.
17	袁军伟，李敏敏，尹勇刚，等.不同砧木嫁接的赤霞珠葡萄对盐胁迫的生理响应［J］.中国农业大学学报，2019，24（8）：48-59.
	Yuan J W，Li M M，Yin Y G，et al.Physiological responses of cabernet sauvign seedlings grafted on different salt-tolerant rootstocks to NaCl stress［J］.Journal of China Agricultural University，2019，24（8）：48-59.
18	董必珍，吕成群，黄宝灵，等.5个种源白花泡桐光合特性比较［J］.安徽农业科学，2018，46（17）：111-113，132.
	Dong B Z，Lv C Q，Huang B L，et al.Comparative analysis of five geographical strains of Paulownia fortunei based on photosynthesis［J］.Journal of Anhui Agricultural Sciences，2018，46（17）：111-113，132.
19	张凌，高翱，张芸香.4个树种对盐碱胁迫的响应［J］.西北林学院学报，2018，33（6）：56-60，242.
	Zhnag L，Gao A，Zhang Y X.Responses of four tree species to saline-alkali stress［J］.Journal of Northwest Forestry University，2018，33（6）：56-60，242.
20	佘汉基，李鹏飞，薛立，等.3种园林植物响应盐胁迫的荧光特性［J］.中南林业科技大学学报，2017，37（8）：54-59.
	She H J，Li P F，Xue L，et al.Chlorophyll fluorescence responses of plant seedlings of three garden species to salt stress［J］.Journal of Central South University of Forestry & Technology，2017，37（8）：54-59.
21	刘建新，王金成，王瑞娟，等.KCl对NaCl胁迫下燕麦幼苗活性氧代谢和渗透溶质积累的影响［J］.植物研究，2015，35（2）：233-239.
	Liu J X，Wang J C，Wang R J，et al.Effects of KCl on active oxygen metabolism and osmotica accumulation in Avena nude L. seedlings under NaCl stress［J］.Bulletin of Botanical Research，2015，35（2）：233-239.
22	杜长霞，李娟，郭世荣，等.外源亚精胺对盐胁迫下黄瓜幼苗生长和可溶性蛋白表达的影响［J］.西北植物学报，2007，27（6）：1179-1184.
	Du C X，Li J，Guo S R，et al.Effects of exogenous spermdine on the growth and soluble protein expression in cucumber seedlings under NaCl stress［J］.Acta Botanica Boreali-Occidentalia Sinica，2007，27（6）：1179-1184.
23	陆瑛，鲁延芳，占玉芳，等.盐胁迫对“宁杞1号”生长表现和生理指标的影响［J］.林业科技通讯，2018，（5）：70-75.
	Lu Y，Lu T F，Zhan Y F，et al.The influence of growth performance and physiological index in salt stress for 3 kinds of tree species［J］.Forest Science and Technology，2018，（5）：70-75.
24	李硕，张毅，姚棋，等.等渗盐胁迫下BR对番茄生长及渗透调节特性的影响［J］.西北农林科技大学学报：自然科学版，2020，48（4）：130-136，145.
	Li S，Zhang Y，Yao Q，et al.Effects of brassinolide on seedling growth and osmotic regulation characteristics of tomato under iso-osmotic salt stress［J］.Journal of Northwest A&F University：Natural Science Edition，2020，48（4）：130-136，145.
25	程淑娟，唐东芹，刘群录.盐胁迫对两种忍冬属植物活性氧平衡的影响［J］.南京林业大学学报：自然科学版，2013，37（1）：137-141.
	Cheng S J，Tang D Q，Liu Q L.Reactive oxygen species homeostasis of two Lonicera species under salt stress［J］.Journal of Nanjing Forestry University：Natural Science Edition，2013，37（1）：137-141.
26	高帆，谢玥，沈妍秋，等.外源褪黑素对氯化钠胁迫下美味猕猴桃实生苗抗氧化物酶和渗透调节物质的影响［J］.浙江农林大学学报，2018，35（2）：291-297.
	Gao F，Xie Y，Shen Y Q，et al.Exogenous melatonin for NaCl stress with antioxidant enzymes and osmotic substances of Aclinidia deliciosa seedlings［J］.Journal of Zhejiang A&F University，2018，35（2）：291-297.

处理 Treatment	受害症状等级Salt-damaged grade
处理 Treatment	1 d	3 d	5 d	7 d
CK	0	0	0	0
T1	0	0	0	0
T2	0	0	0	1
T3	0	1	1	2

	Chl	REC	MDA	SS	SP	Pro	SOD	POD
Chl	1.000
REC	-0.441	1.000
MDA	-0.311	0.709^**	1.000
SS	0.529^*	0.284	0.151	1.000
SP	0.471	0.141	0.197	0.366	1.000
Pro	-0.466	0.248	0.294	-0.207	-0.220	1.000
SOD	0.621^*	-0.387	-0.170	0.248	0.682^**	-0.408	1.000
POD	0.237	0.225	0.479	0.250	0.074	0.019	0.102	1.000

[1]	LI Zi-Yi, HE Zi-Hang, LU Hui-Jun, WANG Yu-Cheng, JI Xiao-Yu. Study on Salt Tolerance of AtUNE12 Gene in Arabidopsis thaliana [J]. Bulletin of Botanical Research, 2020, 40(2): 257-265.
[2]	REN Jia-Bo, WEN Zhi-Bin. Effect of different NaCl contents on Seed Germination and Seeding Growth of Chenopodium glaucum L. [J]. Bulletin of Botanical Research, 2019, 39(5): 716-721.
[3]	NA Xiao-Ying1；WANG Xiu-Wei1*；XU Hao-Yu1；QIAO Li-Na1；WANG Sui1；LIU Gui-Feng1；Kentbayev Yerzhan2；Kentbayeva Botagoz2. Analyzing and Evaluating the Salt Tolerance of Four Kinds of Birch Seedlings. [J]. Bulletin of Botanical Research, 2015, 35(6): 873-882.
[4]	MA Jing1；QU Chun-Pu2；XU Zhi-Ru1,2；YANG Chuan-Ping2；LIU Guan-Jun2. Salt Tolerance of Transgenic Tobacco Containing PsATX* and PsSOD Genes of Polygonum sibiricum Laxm. [J]. Bulletin of Botanical Research, 2015, 35(2): 208-217.
[5]	LIU Jian-Xin；WANG Jin-Cheng；WANG Rui-Juan；JIA Hai-Yan. Effects of KCl on Active Oxygen Metabolism and Osmotica Accumulation in Avena nude L. Seedlings under NaCl Stress [J]. Bulletin of Botanical Research, 2015, 35(2): 233-239.
[6]	WANG Xiu-Wei1；JIA Gui-Mei2；MAO Zi-Jun2*；SUN Tao2；YUAN Xian-Lei2. Effect of NaCl Tolerance on Photosynthetic Physiology and Growth of Seedlings of Three Poplar Clones [J]. Bulletin of Botanical Research, 2015, 35(1): 27-33.
[7]	WANG Yan-Jun;WANG Mei-Yan;HUANG Rong-Rong. Effect of Chitosan Coating on Seed Germination and Salt-tolerance of Brassica napus L. [J]. Bulletin of Botanical Research, 2012, 32(6): 689-694.
[8]	ZHANG Hua;ZHANG Fu-Chun;ZHANG Yu-Liang;ZHANG Bo;CHEN Quan-Jian. Expressional Analysis of MvNHX1 in Medicago varia* Xinmu-1 and Enhancing the Salt Tolerance of Tobacco [J]. Bulletin of Botanical Research, 2011, 31(5): 550-557.
[9]	LIN Ying-Chao;YANG Lei;ZHAO Xiao-Ju;TANG Zhong-Hua. Effects of Increased Endogenous Ethylene on Plant Salt Tolerance in Arabidopsis* Seedlings under Saline Condition [J]. Bulletin of Botanical Research, 2010, 30(6): 703-707.
[10]	LU Zhen-Qiang;LIU Da-Li;LIU Shen-Kui. Elevated Salt Tolerance in Transgenic Tobacco Plants Expressing Cytosolic Ascorbate Peroxidase Genes from Rice(Oryza sativa L.) [J]. Bulletin of Botanical Research, 2010, 30(4): 448-454.
[11]	LIU Jian-Xin;WANG Xin;LI Bo-Ping. Effects of Exogenous Nitric Oxide Donor on the Ascorbate-glutathione Cycle in Peganum multisectum Seedling Leaves under NaCl Stress [J]. Bulletin of Botanical Research, 2010, 30(1): 37-41.
[12]	LI Jing-Nan;LIU Qiang;JIA Zhi-Kuan;WANG Ping-Ping. Effects of Salt Stress on Growth and Physiological Characteristic of Ammopiptanthus mongolicus* seedlings [J]. Bulletin of Botanical Research, 2009, 29(5): 553-558.
[13]	LIU Yun;LI Ran-Hui;WANG Wei-Mao;LIANG Xiang-Yu;ZHENG Yi-Zhi*. Functional Identification of Plant Stress-resistant Protein(LEA3) and Its 22-mer Motifs in Yeast [J]. Bulletin of Botanical Research, 2009, 29(1): 74-79.
[14]	LIU Hui-Min;ZHU Yue-Lin*;CHEN Lei. In Virto Comparision of Ca(NO₃)₂ Tolerance of Different Tomato Cultivars and Rootstock Selfed Lines at Seedling Stage [J]. Bulletin of Botanical Research, 2007, 27(2): 175-181.
[15]	WANG Zhen-Ying, PENG Yong-Kang, ZHENG Jian-Yu. Cloning and sequencing analysis on the cDNA fragment SI₈₀₀ separated from rye which related with salt tolerance [J]. Bulletin of Botanical Research, 2005, 25(4): 427-431.