Effect of Warm Water Soaking on Protective Enzyme Activity and Endogenous Hormones Content of Eucommia ulmoides Seed During Different Stages of Germination

doi:10.7525/j.issn.1673-5102.2020.04.006

Abstract

Abstract: The experiment was conducted to investigate the regulation mechanism of enzymes and endogenous hormones of different stages of Eucommia ulmoides Oliver seed germination, aiming to provide scientific basis for taking effective treatment measures during the different stages of seed dormancy and germination to increase seedling emergence rate. The effects of warm water soaking on the germination of E.ulmoides seeds were studied with room temperature(CK), 40℃, 50℃, 60℃ and 70℃. And the activities of superoxide dismutase(SOD), peroxidase(POD), and malondialdehyde(MDA) content were determined by routine physiological and biochemical methods. Levels of 4 endogenous hormones, such as gibberellin(GA₃), indole acetic acid(IAA), zeatin riboside(ZR) and abscisic acid(ABA) were quantitatively determined by enzyme-linked immunosorbent assay. The result showed that:①The seed germination rate could be increased by soaking seeds with different temperature water, and the germination rate reached its maximum under 50℃ warm water treatment. ②Compared with room temperature water treatment, the activity of SOD and POD in the seeds were increased under 50℃ warm water, and reduced the degree of cytoplasm membrane was oxidized, and the content of MDA was decreased continuously, thus promoted the development of seeds in a direction favorable for germination. In addition, treatment with 50℃ warm water promoted the growth of endogenous GA₃, IAA and ZR in seeds, and decreased the content of endogenous ABA, and the values of GA₃/ABA, IAA/ABA and ZR/ABA were significantly higher than those of room temperature water, which ultimately promoted the germination of E.ulmoides.

Key words: Eucommia ulmoides, germinating stage, SOD, endogenous hormones, MDA, ABA

CLC Number:

Q945.7

WANG Ning, DONG Ying-Ying, YUAN Mei-Li, WANG Ya-Nan, ZENG Sheng-Yun. Effect of Warm Water Soaking on Protective Enzyme Activity and Endogenous Hormones Content of Eucommia ulmoides Seed During Different Stages of Germination[J]. Bulletin of Botanical Research, 2020, 40(4): 523-529.

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