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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (1): 85-92.doi: 10.7525/j.issn.1673-5102.2020.01.013

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Effects of Extracellular adenosine-5'-triphosphate on Cellular damage, H2O2 Content,and Activities of H2O2 Detoxifying Enzymes under Lead Stress

CAO Jia-Xin, DA Meng-Ting, PANG Hai-Long, JIA Ling-Yun, FENG Han-Qing   

  1. College of Life Science, Northwest Normal University, Lanzhou 730070
  • Received:2019-03-13 Online:2020-01-05 Published:2020-02-21
  • Supported by:
    National Natural Science Foundation of China(31870246,31560070);University Scientific Research Project of Gansu Province(2015A-007);Key Research and Development Project of Gansu Province(18YF1NA051);Fundamental Research Funds for the Gansu Universities of Gansu Provincial Department of Finance;Youth Innovation Team of Northwest Normal University

Abstract: The extracellular adenosine-5'-triphosphate is an important signaling molecule for plant cells. By using the tobacco(Nicotiana tabacum L.cv.Bright Yellow-2) cell suspension cultures, we studied the effects of extracellular adenosine-5'-triphosphate on the cellular damage, H2O content, and the activities of H2O detoxifying enzymes underlead stress. With the increase of concentrations of Pb(NO3)2(from 30-400 μmol·L-1), the levels of the extracellular adenosine-5'-triphosphate were decreased, whilethe levels of the intracellular adenosine-5'-triphosphate and cellular damage were increased. The content of H2O2 and the activity of the CAT(catalaseenzyme) were increased and reached their maximum at 200 μmol·L-1Pb(NO3)2, while the activityof the POD(peroxide enzyme) was decreased, with the increase of concentrations of Pb(NO3)2. Compared with the cells under lead(200 μmol·L-1) stress, the lead-stressed cells treated with exogenous adenosine-5'-triphosphate(30 μmol·L-1) had lower levels of cellular damage, H2O2 content, andthe activity of CAT, but had higher level of the activity of POD. Therefore, the cellular damage, H2O content, and the activities of H2O detoxifying enzymesunderlead stress could beregulated by extracellular adenosine-5'-triphosphate.

Key words: cellular damage, extracellular adenosine-5'-triphosphate, lead stress, H2O2, H2O detoxifying enzymes

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